ABSTRACT
(2-Hydroxyethyl) dimethylsulfoxonium chloride (1), the well-known causative agent of Dogger Bank Itch, has been isolated as a cytotoxic constituent from the marine sponge Theonella aff. mirabilis. The structure of 1 was determined by spectral and X-ray crystallographic analyses.
Subject(s)
Chlorides/isolation & purification , Dermatitis, Contact/etiology , Porifera/chemistry , Sulfonium Compounds/isolation & purification , Animals , Chlorides/chemistry , Chlorides/pharmacology , Crystallography, X-Ray , Cytotoxins/chemistry , Inhibitory Concentration 50 , Magnetic Resonance Spectroscopy , Mice , Models, Chemical , Models, Molecular , Sulfonium Compounds/chemistry , Sulfonium Compounds/pharmacology , Tumor Cells, CulturedABSTRACT
A recombinant CHO cell line (GT19) secreting a high level of human growth hormone (hGH) was constructed with amplification of the introduced hGH gene. The cells grew well in the alpha MEM medium supplemented with 5% dialyzed fetal calf serum (dFCS), but not with less than 1% dFCS. Therefore we examined various medium components and obtained an improved medium which supported cell growth at low serum concentrations. The production of hGH by the cells was also enhanced in this medium.
Subject(s)
Growth Hormone/biosynthesis , Transfection , Animals , CHO Cells , Cell Division/drug effects , Cell Line , Cricetinae , Culture Media , Culture Media, Serum-Free , Culture Techniques/methods , Growth Hormone/genetics , Growth Hormone/pharmacology , Humans , Plasmids , Recombinant Proteins/biosynthesis , Recombinant Proteins/pharmacology , Restriction MappingABSTRACT
A number of vibrio strains isolated from marine water produced high units of phytohemagglutinin-like agglutinins. Sugar specificity of the hemagglutinins was different from that of the sugar-binding bacterial toxins and that of the sugar-binding pili on the bacterial cell surfaces.
