ABSTRACT
Objective:To explore the efficacy of Liuwei Nengxiao capsule combined with fecal bacteria transplantation on chronic functional constipation with spleen Qi deficiency. Method:A total of 129 patients with chronic functional constipation of spleen Qi deficiency treated in Affiliated Hospital of Shandong University of Traditional Chinese Medicine were randomly divided into three groups by the computer numerical table method： fecal bacteria transplantation group， Liuwei Nengxiao capsule group， and Liuwei Nengxiao capsule combined with fecal bacteria transplantation group， with 43 cases in each group. Fecal bacteria transplantation group was treated with fecal bacteria， Liuwei Nengxiao capsule group was treated with Liuwei Nengxiao capsule， and Liuwei Nengxiao capsule combined with fecal bacteria transplanted group was treated with Liuwei Nengxiao capsule combined with fecal bacteria. The efficacy of the 3 groups was compared. Result:After treatment， the frequency and amplitude of intestinal electricity increased significantly in three parts， with statistically significant differences from before treatment （P<0.05）. After treatment， the frequency and amplitude of the transverse colon， sigmoid colon， and descending colon of patients in Liuwei Nengxiao capsule combined with faecal transplantation group were higher than those in faecal transplantation group and Liuwei Nengxiao capsule group （P<0.05）. After treatment， substance P （SP） and motilin （MTL） of Liuwei Nengxiao capsule combined with faecal transplantation group were higher than those of faecal transplantation group and Liuwei Nengxiao capsule group， while nitric oxide （NO） and vasoactive intestinal peptide （VIP） were lower than those of faecal transplantation group and Liuwei Nengxiao capsule group （P<0.05）. The number of bifidobacteria and lactobacillus after treatment was higher in three groups than before treatment （P<0.05）. The numbers of yeast and enterobacteria were lower than before treatment （P<0.05）. After treatment， the number of bifidobacteria and lactobacillus in Liuwei Nengxiao capsule combined with faecal transplantation group was higher than that in faecal transplantation group and Liuwei Nengxiao capsule group， whereas the numbers of yeast， and enterobacteria were all lower than those in bacterial transplantation group and Liuwei Nengxiao capsule group （P<0.05）. After treatment， the BSFS scores of three groups were higher than before the treatment， while Wexner continence grading scale （Wexner） constipation score and patient assessment of constipation quality of life questionnaire （PAC-QOL） score were lower than before treatment （P<0.05）. Bristol stool form scale （BSFS） score of Liuwei Nengxiao capsule combined with fecal bacteria transplantation group was higher than that of fecal bacteria transplantation group and Liuwei Nengxiao capsule group. Wexner constipation score and PAC-QOL score were lower than those of fecal bacteria transplantation group and Liuwei Nengxiao capsule group （P<0.05）. Conclusion:Fecal bacteria transplantation combined with Liuwei Nengxiao capsule can effectively promote the recovery of intestinal electrical function， improve the intestinal flora balance， reduce the intestinal oxidative stress response， and promote the disappearance of patients' symptoms in the treatment of elderly chronic functional constipation， and thus is worth further promotion in clinic application.
ABSTRACT
Protein tyrosine phosphatases (PTPs) are enzymes that catalyze protein tyrosine dephosphorylation of which Src homology phosphatase-1 (SHP-1) is one of the best-validated, a widely distributed intracellular tyrosine phosphatase that contains two SH2 domains. Down regulation of SHP-1 tyrosine phosphatases was significantly increased sensitivity to insulin in insulin signaling pathway. Through in vitro enzymatic reaction kinetics experiment, we found that the extract of Perilla stem was a potential inhibitor to δSHP-1, the catalytic domain of SHP-1 protein tyrosine phosphatase, and its IC(50) was 4ug/ml, and was more sensitive towards SHP-1than other PTPs, which indicated that SHP-1 might be a target of the extract of Perilla stem. It can strengthened the level of tyrosine phosphorylation of insulin receptor (IR) and extracellular signal-regulated protein kinase (ERK) in HepG2 cells, and then activated the insulin signaling pathway through inhibiting the protein phosphorylation of SHP-1. These results demonstrated that the extract of Perilla stem could play an important role for diabetes treatment through inhibiting the level of SHP-1 in insulin signaling pathway.
