ABSTRACT
A time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of hexoestrol (HES) residues in animal tissues. The limit of detection (LOD) was determined to be 0.02 ng g(-1) and the limit of quantification (LOQ) was less than 0.12 ng g(-1). The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of market chicken muscle tissues and confirmed by high-performance liquid chromatography and tandem mass spectrometry (LC-MS-MS). This proposed technique could be applied to routine residue analysis.
Subject(s)
Drug Residues/analysis , Fluoroimmunoassay/standards , Hexestrol/analysis , Meat/analysis , Animals , Antibodies , Chickens , Chromatography, Liquid/standards , Fluoroimmunoassay/methods , Muscles/chemistry , Tandem Mass Spectrometry/standardsABSTRACT
Fourteen kinds of ethyl ester of fatty acids form Hai Haozi(Sargassum pallidum) are analysed by GC methods.They are identified by comparision of their rentention time with those of known standards and quantitated by using an internal standard.It shows that this drug contains lots of saturated and unsaturated fatty acids.The analytical resulits may provide material basis for futher research of Hai Haozi.
