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1.
Sci Rep ; 12(1): 15833, 2022 09 22.
Article in English | MEDLINE | ID: mdl-36138123

ABSTRACT

Following the introduction of pneumococcal conjugate vaccines (PCVs), the rate of invasive pneumococcal disease (IPD) declined, however, IPDs replaced by serotypes that are not included in the vaccine have emerged. We describe the epidemiology of IPD in South Korea over a 4.5-year period, encompassing the impact following introduction of PCV10/13 and PPSV23 into the public immunization program, and assess serotype dynamics in pediatric and adult population. This was a nationwide, retrospective review of surveillance of all IPD cases in Korea between September 2014 to December 2019. We analyzed VT13 (serotypes included in 13-valent conjugate vaccine) and NVT (nonvaccine type) cases by age, sex, IPD type, vaccination status, and deaths. A total of 893 cases with serotype data were included; 306 (34%) VT13 cases and 587 (66%) NVT cases. Serotype 3 (n = 155) was the most common VT13 serotype, followed by serotypes 19A (n = 70) and 14 (n = 28). Among the NVTs, serotype 10A (n = 74) was the most common serotype, followed by serotypes 23A (n = 60) and 34 (n = 58). Persons who had PCV13 vaccination were at lower risk (aOR = 0.11, 95% CI 0.02-0.73, P = 0.022) of death compared to unvaccinated persons. Introduction of PCV10/13 and PPSV23 vaccination program has had different impacts on the serotype-specific IPD across age groups. The most common serotypes included serotypes 3 and 19A (VT13), and 10A, 23A, and 34 (NVT). Our findings suggest continued monitoring in the midst of new vaccine development, and a need to develop novel strategies to mitigate the IPDs from emerging pneumococcal serotypes.


Subject(s)
Pneumococcal Infections , Pneumococcal Vaccines , Adult , Child , Humans , Immunization Programs , Infant , Pneumococcal Infections/epidemiology , Pneumococcal Infections/prevention & control , Pneumococcal Vaccines/therapeutic use , Republic of Korea/epidemiology , Serogroup , Streptococcus pneumoniae , Vaccination , Vaccines, Conjugate
2.
BMC Microbiol ; 16(1): 206, 2016 09 06.
Article in English | MEDLINE | ID: mdl-27599615

ABSTRACT

BACKGROUND: Bacterial two-component regulatory systems (TCRS) are associated with the expression of virulence factors and antibiotic susceptibility. In Staphylococcus aureus, 16 TCRS types have been identified. The histidine kinase/response regulator SAV1321/SAV1322 in the S. aureus shares considerable homology with the TCRS DesKR in Bacillus subtilis. However, a function for the SAV1322 locus has not yet been assigned. RESULTS: Deletion of the SAV1322 locus in S. aureus results in reduced growth when cultured under low (25 °C) and high (46 °C) temperature conditions. The sav1322 deletion mutant is more tolerant to oxidative stress in vitro and is less pathogenic in a murine infection model when compared with wild-type parent strain Mu50. Furthermore, the sav1322 mutant exhibits lower MICs for gentimicin, tetracyclines and glycopeptides, increased autolysis, and a thinner cell wall when compared with the wild-type strain. Microarray and proteomic analyses show that the expression of cell-wall-associated genes glmS and murZ are lower, and the expression of heat shock and stress-related genes (hrcA, ctsR, dnaK, dnaJ, grpE, clpB, and clpC) are higher in the sav1322 mutant when compared with the wild-type strain. In addition, the sav1322 mutant displays altered expression of proteins involved in carbohydrate/energy metabolism, cell wall metabolism, and stress or heat shock response, as well as other metabolic processes including lipid metabolism, amino acid biosynthesis, purine or pyrimidine metabolism, transcription, and protein biosynthesis. CONCLUSIONS: The S. aureus SAV1322 locus plays a pronounced role in temperature adaptation, antibiotic resistance, and virulence by regulating a wide range of genes and proteins involved in metabolism and stress tolerance.


Subject(s)
Gene Expression Regulation, Bacterial/physiology , Genes, Bacterial/physiology , Genetic Structures/genetics , Genetic Structures/physiology , Genomics , Proteomics , Staphylococcus aureus/genetics , Animals , Bacterial Proteins/genetics , Base Sequence , Cell Wall/metabolism , Disease Models, Animal , Drug Resistance, Bacterial/genetics , Drug Resistance, Microbial/genetics , Female , Gene Deletion , Gene Knockout Techniques , Genes, Bacterial/drug effects , Heat-Shock Proteins/genetics , Histidine Kinase/pharmacology , Mice , Mice, Inbred BALB C , Microscopy, Electron, Transmission , Murinae , Oxidative Stress , Phenotype , Staphylococcal Infections/microbiology , Staphylococcus aureus/cytology , Staphylococcus aureus/growth & development , Staphylococcus aureus/metabolism , Stress, Psychological/genetics , Temperature , Virulence Factors/genetics
3.
Genome Announc ; 4(3)2016 Jun 16.
Article in English | MEDLINE | ID: mdl-27313284

ABSTRACT

We report here the draft genome sequences of four vancomycin-intermediate Staphylococcus aureus (VISA) strains from South Korean hospitals participating in a nationwide laboratory surveillance program for vancomycin-intermediate and vancomycin-resistant Staphylococcus aureus All strains harbor mutations in the walKR, graSR, and/or rpoB genes that are known frequently mutated determinants of VISA.

4.
Microb Drug Resist ; 22(7): 538-544, 2016 Oct.
Article in English | MEDLINE | ID: mdl-26982169

ABSTRACT

The small-colony variant (SCV) phenotype of Staphylococcus aureus is associated with intracellular persistence and reduced antimicrobial susceptibility, which can lead to therapeutic failure. Since SCVs grow slowly and have a confusing morphology, the identification of infections due to SCV is difficult. We have identified SCVs in two patients who presented with persistent methicillin-resistant Staphylococcus aureus (MRSA) bacteremia complicated by surgical site infections after cardiothoracic surgery. Nine blood isolates were collected from the two patients for species identification, antimicrobial susceptibility testing, and phenotypic and genotypic characterization. Colonies on Columbia blood agar were pinpoint, nonpigmented, nonhemolytic, and reverted to normal colonies after 48 hr of incubation on Schaedler agar. Auxotrophy assays revealed hemin dependence. Susceptibility to vancomycin (minimal inhibitory concentrations 1.0 µg/mL) was confirmed by E-test and broth microdilution test. All the isolates were identified as MRSA by multiplex polymerase chain reaction specific for the mecA, femA, and 16S rRNA genes, and all had the same genotype: Multilocus sequence typing ST5, SCCmec type II, agr type II, and spa type t2460. Moreover pulsed-field gel electrophoresis typing revealed that all nine isolates belonged to the same clone. Mutations in the relA gene were not found, and none of the isolates was identified as hVISA by population analysis profiling-AUC ratio. A high level of suspicion is required to detect SCVs, and although it is not common, the possibility of the SCV phenotype has to be considered in persistent S. aureus bacteremia.


Subject(s)
Bacteremia/microbiology , Genes, Bacterial , Methicillin-Resistant Staphylococcus aureus/genetics , Staphylococcal Infections/microbiology , Surgical Wound Infection/microbiology , Aged , Anti-Bacterial Agents/pharmacology , Bacteremia/drug therapy , Bacteremia/pathology , Bacterial Typing Techniques , Clone Cells , Electrophoresis, Gel, Pulsed-Field , Genotype , Humans , Male , Methicillin-Resistant Staphylococcus aureus/classification , Methicillin-Resistant Staphylococcus aureus/drug effects , Methicillin-Resistant Staphylococcus aureus/isolation & purification , Multilocus Sequence Typing , Phenotype , Recurrence , Staphylococcal Infections/drug therapy , Staphylococcal Infections/pathology , Surgical Wound Infection/drug therapy , Surgical Wound Infection/pathology , Vancomycin/pharmacology
5.
Diagn Microbiol Infect Dis ; 80(2): 148-50, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25139845

ABSTRACT

Of 18 vanA-positive vancomycin-susceptible Enterococcus faecium isolates, vanRS in the vanA cluster was detected in all isolates, while vanHAX was detected in only 2 isolates. Following exposure to glycopeptides, 22.2% of vancomycin-susceptible E. faecium (VSE) converted into vancomycin-resistant E. faecium. The vanA cluster of the revertant mutant was transferred to the VSE isolates.


Subject(s)
Anti-Bacterial Agents/pharmacology , Bacterial Proteins/genetics , Carbon-Oxygen Ligases/genetics , Enterococcus faecium/drug effects , Enterococcus faecium/genetics , Vancomycin Resistance , Vancomycin/pharmacology , Enterococcus faecium/isolation & purification , Genes, Bacterial , Gram-Positive Bacterial Infections/microbiology , Humans , Multigene Family
6.
Osong Public Health Res Perspect ; 4(5): 225-32, 2013 Oct.
Article in English | MEDLINE | ID: mdl-24298437

ABSTRACT

OBJECTIVES: To investigate the biofilm-forming related factors against MRSA bloodstream isolates and evaluates their clinical features and treatment outcomes by biofilm production. METHODS: We collected 126 consecutive methicillin-resistant Staphylococcus aureus (MRSA) causing blood stream infections (BSIs) at 10 tertiary hospitals from 2007 to 2009. We investigated biofilm-forming ability using a microtiter plate assay, and molecular characteristics including multilocus sequence typing, staphylococcal cassette chromosome mec and accessory gene regulator types. We compared the clinical characteristics and outcomes of patients infected with biofilm-forming and non-biofilm-forming MRSA isolates. RESULTS: Of the 126 samples, 86 (68.3%), including 5 strong level (OD570 ≥ 1.0) and 81 weak level (0.2 ≤ OD570 < 1.0), had biofilm-forming capacity. Detection of fibronectinbinding protein in biofilm-forming strains was significantly higher than biofilm non-forming ones (p = 0.001) and three enterotoxin genes (sec-seg-sei) islands had a high frequency regardless of biofilm production. However, biofilm-forming strains were more likely to be multidrug resistant (three or more non-ß-lactam antibiotics) than biofilm non-forming ones [79.2% vs. 59.2%, p = 0.015, odds ratio (OR) 2.629, 95% confidence interval (CI) 1.92-5.81]. Clinical features of patients with BSIs caused by biofilm-forming MRSA strains were more likely to be hospital onset [77.9% vs. 60.0%, p = 0.024, OR 2.434, 95% CI 1.11-5.33) and more frequently occurred in patients with use of invasive devices [85.7% vs. 61.2%, p = 0.002, OR 3.879, 95% CI 1.61-8.97]. The other clinical features were compared with the clinical outcomes of the two groups and were not significant (p > 0.05). CONCLUSION: Biofilm-forming MRSA strains showed higher frequency of fnbB gene than biofilm non-forming ones and more incidence rates on particular genotypes. And, their patient's features were not significantly different between two groups in this study, except for several clinical factors.

7.
J Med Microbiol ; 62(Pt 9): 1338-1342, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23741020

ABSTRACT

This study analysed the characteristics and genetic similarity of recent Klebsiella pneumoniae carbapenemase (KPC-2)-producing Klebsiella pneumoniae isolates from Korea. Recent laboratory surveillance detected an increase in carbapenemase-producing Enterobacteriaceae in Korea. A total of 6 KPC-2-producing K. pneumoniae were identified from 277 Enterobacteriaceae clinical isolates. All were sequence type (ST) 258 and they had the same pulsotype. They had high MICs for carbapenems and multi-drug resistance. TEM-1, SHV-11 and OXA type ß-lactamases were detected in all isolates, whereas CTX-M type ß-lactamases and plasmid-mediated AmpC ß-lactamase (PABL) were not present. A conjugation experiment failed, but blaKPC-2-harbouring plasmids from the six isolates were used to transform Escherichia coli DH5-α by electroporation. Each of the transformants harboured a blaKPC-2-positive approximately 95 kb plasmid, which was typed in the IncFII incompatibility group and co-harboured TEM-1 and OXA-9 ß-lactamases. They shared the same restriction profile. This study confirms the emergence of clonal ST258 KPC-2-producing K. pneumoniae in some regions of Korea.


Subject(s)
Klebsiella Infections/diagnosis , Klebsiella Infections/epidemiology , Klebsiella pneumoniae/isolation & purification , beta-Lactamases/isolation & purification , Anti-Bacterial Agents/pharmacology , Carbapenems/pharmacology , Conjugation, Genetic , Drug Resistance, Multiple, Bacterial , Genotype , Humans , Klebsiella Infections/enzymology , Klebsiella pneumoniae/drug effects , Klebsiella pneumoniae/genetics , Microbial Sensitivity Tests , Plasmids/genetics , Plasmids/metabolism , Prevalence , Republic of Korea/epidemiology , Tertiary Care Centers , beta-Lactamases/genetics
9.
Tuberc Res Treat ; 2013: 108401, 2013.
Article in English | MEDLINE | ID: mdl-23606961

ABSTRACT

Many laboratories validate DST of the second-line drugs by BACTEC MGIT 960 system. The objective of this study is to evaluate the critical concentration and perform DST for the 2nd line drugs. We evaluated 193 clinical strains of M. tuberculosis isolated from patients in South Korea. Testing the critical concentration of six second-line drugs was performed by MGIT 960 and compared with L-J proportion method. The critical concentration was determined to establish the most one that gave the difference between drug resistance and susceptibility in MGIT960 system. Good agreement of the following concentrations was found: Concordance was 95% for 0.5 µ g/mL of moxifloxacin; 93.6%, 1.0 µ g/mL of levofloxacin; 97.5%, 2.5 µ g/mL of kanamycin; 90.6%, 2.5 µ g/mL of capreomycin; 86.2%, 5.0 µ g/mL of ethionamide; and 90.8%, 2.0 µ g/mL of ρ-aminosalicylic acid. The critical concentrations of the four drugs, moxifloxacin, levofloxacin, kanamycin, and capreomycin, were concordant and reliable for testing 2nd line drug resistance. Further study of ethionamide and ρ -aminosalicylic acid is required.

10.
J Microbiol ; 51(2): 160-5, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23625215

ABSTRACT

Vancomycin intermediate Staphylococcus aureus (VISA) strains are increasingly prevalent in the hospital setting, and are of major concern in the treatment of methicillin-resistant S. aureus infections. Multiple mutations in vancomycin-susceptible S. aureus (VSSA) strains likely led to the emergence of VISA, and point mutations in the agr, orf1, yvqF, vraSR, graSR, and tcaRAB genes of VISA strains have been shown to contribute to glycopeptide resistance. Therefore, we investigated point mutations in these genes from 87 VISA and 27 VSSA clinical strains isolated from Korean hospitals. All strains were assigned an agr type (I, II, or III) on the basis of multiplex PCR, with the majority of VISA strains belonging to agr groups I and II. Sequencing revealed amino acid changes in vraS from VISA strains which were not present in the VSSA strains. The E59D substitution in the vraR gene occurred in 36.3% of VSSA/agrI and 92.7% of VISA/agrI strains, suggesting that this mutation associated with emergence of VISA/agrI strains. VISA strains were classified into 31 mutation patterns according to mutations in the yvqF, vraSR, graSR, and tcaRAB genes. In addition, the mutation patterns were correlated with agr and sequence type (ST). The most prevalent pattern included agr type I (ST 72) strains with E59D (vraR), L26F and T224I (graS), D148Q (graR), and L218P, R283H and G312D (tcaA) amino acid substitutions. The minimum inhibitory concentration (MIC) range of mutation pattern 5 toward oxacillin and imipenem was much lower than that of patterns 6 and 24. These results improve our understanding of emergence of VISA strains.


Subject(s)
Amino Acid Substitution , Anti-Bacterial Agents/pharmacology , Bacterial Proteins/genetics , DNA-Binding Proteins/genetics , Staphylococcal Infections/microbiology , Staphylococcus aureus/genetics , Vancomycin Resistance , Vancomycin/pharmacology , Humans , Mutation, Missense , Point Mutation , Republic of Korea/epidemiology , Staphylococcal Infections/epidemiology , Staphylococcus aureus/drug effects , Staphylococcus aureus/isolation & purification
11.
Yonsei Med J ; 54(1): 177-82, 2013 Jan 01.
Article in English | MEDLINE | ID: mdl-23225816

ABSTRACT

PURPOSE: The increasing prevalence and global spread of carbapenem-resistant Acinetobacter baumannii (A. baumannii) has become a serious problem. The aim of this study was to investigate molecular and epidemiological characteristics of carbapenem-resistant A. baumannii isolates collected from Korean non-tertiary hospitals. MATERIALS AND METHODS: Thirty six non-duplicated carbapenem-resistant A. baumannii isolates were collected from 17 non-tertiary hospitals in Korea between 2004 and 2006. Isolates were typed by multilocus sequence typing and repetitive-sequence-based PCR (rep-PCR). Detection of genes encoding OXA carbapenemase and their relationship with ISAba1 was performed by PCR. RESULTS: Two clones were prevalent among 36 isolates: ST69 (17 isolates, 47.2%) and ST92 (19 isolates, 52.8%). Rep-PCR patterns were diverse and revealed that all isolates were clustered into eight band patterns. The ISAba1-activated blaOXA-23-like and ISAba1-activated blaOXA-51-like genes were prevalent among the carbapenem- resistant A. baumannii isolates. CONCLUSION: The class D ß-lactamase genes of A. baumannii were distributed nationwide in non-tertiary Korean hospitals.


Subject(s)
Acinetobacter Infections/microbiology , Acinetobacter baumannii/genetics , Carbapenems/therapeutic use , Drug Resistance, Bacterial , Acinetobacter Infections/epidemiology , Acinetobacter baumannii/classification , Anti-Bacterial Agents/therapeutic use , Bacterial Typing Techniques , DNA, Bacterial/analysis , Hospitals , Humans , Microbial Sensitivity Tests , Molecular Epidemiology , Multilocus Sequence Typing , Polymerase Chain Reaction , Prevalence , Republic of Korea , beta-Lactamases/genetics
13.
Osong Public Health Res Perspect ; 4(6): 293-300, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24524017

ABSTRACT

OBJECTIVES: The proteomic analysis of voriconazole resistant Candida glabrata strain has not yet been investigated. In this study, differentially expressed proteins of intracellular and membrane fraction from voriconazole-susceptible, susceptible dose-dependent (S-DD), resistant C. glabrata strains were compared with each other and several proteins were identified. METHODS: The proteins of intracellular and membrane were isolated by disrupting cells with glass bead and centrifugation from voriconazole susceptible, S-DD, and resistant C. glabrata strains. The abundance of expressed proteins was compared using two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis and proteins showing continuous twofold or more increase or reduction of expression in resistant strains compared to susceptible and S-DD strain were analyzed by liquid chromatography/mass spectrometry-mass spectrometry method. RESULTS: Of 34 intracellular proteins, 15 proteins showed expression increase or reduction (twofold or more). The identified proteins included regulation, energy production, carbohydrate transport, amino acid transport, and various metabolism related proteins. The increase of expression of heat shock protein 70 was found. Among membrane proteins, 12, 31 proteins showed expression increase or decrease in the order of susceptible, S-DD, and resistant strains. This expression included carbohydrate metabolism, amino acid synthesis, and response to stress-related proteins. In membrane fractions, the change of expression of 10 heat shock proteins was observed, and 9 heat shock protein 70 (Hsp70) showed the reduction of expression. CONCLUSION: The expression of Hsp70 protein in membrane fraction is related to voriconazole resistant C. glabrata strains.

14.
J Med Microbiol ; 61(Pt 10): 1428-1434, 2012 Oct.
Article in English | MEDLINE | ID: mdl-22820692

ABSTRACT

The aim of this study was to investigate the molecular characteristics of induced vancomycin resistance in Staphylococcus haemolyticus. Autolytic properties and phenotypic characteristics of passage-selected vancomycin-resistant S. haemolyticus strains were examined. In addition, expression of autolysis-related genes (atl, lrgAB, sarA and lytS) was investigated using the RNase protection assay (RPA). The RPA results indicated that only the expression of the atl gene was significantly upregulated (2.5- to 6-fold increase) in vancomycin-intermediate and vancomycin-resistant strains. The vancomycin-resistant strains exhibited lower expression of murein hydrolase proteins and reduced autolytic activity compared with the parent strain. In addition, a reduced growth rate, cell wall thickening and higher survival rate in the presence of lysostaphin were observed in vancomycin-intermediate and vancomycin-resistant induced strains compared with the parent strain. In conclusion, altered autolytic properties, in particular upregulation of the atl gene, may contribute to vancomycin resistance in S. haemolyticus.


Subject(s)
Anti-Bacterial Agents/pharmacology , Bacteriolysis/physiology , Staphylococcus haemolyticus/drug effects , Vancomycin Resistance/physiology , Vancomycin/pharmacology , Autolysis , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Bacteriolysis/genetics , Detergents/pharmacology , Humans , Microscopy, Electron, Transmission , Mutation , Octoxynol/pharmacology , Republic of Korea/epidemiology , Reverse Transcriptase Polymerase Chain Reaction , Staphylococcal Infections/epidemiology , Staphylococcal Infections/microbiology , Staphylococcus haemolyticus/genetics , Staphylococcus haemolyticus/ultrastructure , Vancomycin Resistance/genetics
15.
J Med Microbiol ; 61(Pt 8): 1121-1128, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22538996

ABSTRACT

This study was conducted to investigate the molecular characteristics and genetic relatedness of vancomycin-resistant Enterococcus faecium (VREF) isolates obtained from humans and poultry in Korea. A total of 147 VREF isolates from humans (71 clinical isolates) and poultry (76 isolates) in Korea were compared with respect to their antibiotic susceptibilities, organization of the Tn1546 transposon element, detection of virulence genes (esp and hyl), pulsed-field gel electrophoresis (PFGE) typing and multilocus sequence typing (MLST). All of the human and poultry isolates had the vanA gene and 11.3% (8/71) of the clinical isolates showed the VanB phenotype/vanA genotype. PCR mapping of the Tn1546 elements was different for isolates of the two groups: human isolates were classified into five transposon types, whereas all poultry isolates were identical to Tn1546 of E. faecium strain BM4147. The esp gene was detected in both human (93.0%, 66/71) and poultry (26.3%, 20/76) isolates, as was the hyl gene (human isolates: 80.3%, 57/71; poultry isolates: 26.3%, 20/76). Using MLST, the 71 human isolates could be divided into ten sequence types (STs) belonging to clonal complex (CC) 17 (except for one singleton). The 76 poultry isolates were categorized into 14 STs and 88.2% (67/76) of the poultry isolates belonged to CC26. PFGE typing of the human isolates demonstrated diverse PFGE profiles among the strains. However, the PFGE patterns of the poultry isolates were possibly related to the strains collected from individual farms. These data suggest that epidemic clonal groups of human and poultry VREFs in Korea have evolved through different evolutionary processes.


Subject(s)
Enterococcus faecium/classification , Enterococcus faecium/genetics , Gram-Positive Bacterial Infections/microbiology , Gram-Positive Bacterial Infections/veterinary , Vancomycin Resistance , Animals , DNA Fingerprinting , DNA Transposable Elements , DNA, Bacterial/genetics , Enterococcus faecium/drug effects , Enterococcus faecium/isolation & purification , Gene Order , Genes, Bacterial , Humans , Korea/epidemiology , Microbial Sensitivity Tests , Molecular Epidemiology , Multilocus Sequence Typing , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Poultry
16.
Int J Antimicrob Agents ; 39(4): 300-4, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22325122

ABSTRACT

The present study aimed to describe the prevalence and molecular epidemiology of metallo-ß-lactamase (MBL)-producing Pseudomonas aeruginosa isolates obtained from non-tertiary care hospitals and geriatric hospitals in South Korea. Of the 644 isolates, 224 were carbapenem-resistant, amongst which 41 (18.3%) were MBL-producers and the major MBL type was IMP-6 (35 isolates). IMP-6-producing isolates were multidrug-resistant and showed higher minimum inhibitory concentrations for meropenem than imipenem. All of the IMP-6-producing isolates had class 1 integrons with amplification sizes of 4.5 kb/5.5 kb (34 isolates) or 3.0 kb (1 isolate); 4.5 kb/5.5 kb integrons had bla(IMP-6)-qac-aacA4-bla(OXA-1)-aadA1 (5.5 kb) and aadB-cmlA-bla(OXA-10)-aadA1 (4.5 kb). Pulsed-field gel electrophoresis (PFGE) analysis indicated that all IMP-6-producing P. aeruginosa from various geographic areas had nearly identical patterns with >85% similarity. All IMP-6-producing isolates showed high genetic similarity to those obtained from tertiary care hospitals and had the same integron type, indicating the spread of these strains to the three types of hospitals nationwide. These data show the wide spreading of clonally related IMP-6-producing P. aeruginosa (sequence type 235) through tertiary, non-tertiary and geriatric hospitals in South Korea. Continuous monitoring and thorough infection control should be performed in all types of hospitals to prevent further spreading of MBL-producing P. aeruginosa.


Subject(s)
Cross Infection/microbiology , DNA, Bacterial/genetics , Pseudomonas Infections/transmission , Pseudomonas aeruginosa/genetics , beta-Lactamases/metabolism , Drug Resistance, Multiple, Bacterial , Electrophoresis, Gel, Pulsed-Field , Genes, Bacterial , Hospitals , Humans , Integrons , Microbial Sensitivity Tests , Molecular Epidemiology , Multilocus Sequence Typing , Multiplex Polymerase Chain Reaction , Prevalence , Pseudomonas Infections/microbiology , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/isolation & purification , Pseudomonas aeruginosa/metabolism , Republic of Korea/epidemiology , beta-Lactamases/genetics
17.
Osong Public Health Res Perspect ; 3(2): 74-8, 2012 Jun.
Article in English | MEDLINE | ID: mdl-24159494

ABSTRACT

OBJECTIVES: Candida glabrata is one of the most common causes of Candida bloodstream infections worldwide. Some isolates of C glabrata may be intermediately resistant to azoles, with some strains developing resistance during therapy or prophylaxis with fluconazole. In this study, we used a proteomic approach to identify differentially expressed proteins between fluconazoleresistant and -susceptible strains. METHODS: Membrane and cellular proteins were extracted from fluconazolesusceptible and fluconazole-resistant C glabrata strains. Differentially expressed proteins were compared using two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Proteins with >1.5-fold difference in expression were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). RESULTS: A total of 65 proteins were differentially expressed in the cellular and membrane fractions. Among the 39 cellular proteins, 11 were upregulated and 28 were downregulated in fluconazole-resistant strains in comparison with fluconazole-susceptible strains. In the membrane fraction, a total of 26 proteins were found, of which 19 were upregulated and seven were downregulated. A total of 31 proteins were identified by LC-MS/MS that are involved in glycolysis, carbohydrate transport, energy transfer, and other metabolic pathways. Heat shock proteins were identified in various spots. CONCLUSION: Heat shock and stress response proteins were upregulated in the membrane fraction of the fluconazole-resistant C glabrata strain. Compared with susceptible strains, fluconazole-resistant strains showed increased expression of membrane proteins and decreased expression of cellular proteins.

18.
J Med Microbiol ; 61(Pt 3): 345-352, 2012 Mar.
Article in English | MEDLINE | ID: mdl-22016559

ABSTRACT

Reduced vancomycin susceptibility in Staphylococcus aureus can cause serious problems relating to treatment failure and persistent infection. We investigated vancomycin susceptibility, genetic relationships and transcriptional changes of the accessory gene regulator (agr) in vancomycin-intermediate S. aureus (VISA) strains isolated from South Korea compared with vancomycin-susceptible S. aureus (VSSA) strains. Molecular characterization, population analysis profiling, agr sequencing and transcriptional profiling of RNAIII by real-time RT-PCR were performed. Of 16 VISA strains tested, eight exhibited ST5, agr II and type II SCCmec. The others exhibited ST239, agr I and type III SCCmec. A point mutation in AgrA (Asp8Gly or Ile238Lys) was found in only five VISA strains; no mutations were detected in the other strains. However, RNAIII levels markedly decreased in all VISA strains (mean of 1.39-fold change) compared with the VSSA strains (31.51-fold change) in late-exponential phases (P<0.0001). The downregulation of RNAIII could be an important genetic event in the VISA strains, regardless of the presence or absence of the agr mutation.


Subject(s)
Bacterial Proteins/biosynthesis , Bacterial Proteins/genetics , Gene Expression Regulation, Bacterial , Mutation, Missense , Staphylococcus aureus/drug effects , Staphylococcus aureus/physiology , Trans-Activators/biosynthesis , Trans-Activators/genetics , Vancomycin Resistance , Anti-Bacterial Agents/pharmacology , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Down-Regulation , Gene Expression Profiling , Humans , Microbial Sensitivity Tests , Molecular Sequence Data , Molecular Typing , Republic of Korea , Sequence Analysis, DNA , Staphylococcal Infections/microbiology , Staphylococcus aureus/genetics , Staphylococcus aureus/isolation & purification , Vancomycin/pharmacology
19.
Osong Public Health Res Perspect ; 2(3): 164-70, 2011 Dec.
Article in English | MEDLINE | ID: mdl-24159468

ABSTRACT

OBJECTIVES: This study investigated the fluoroquinolone-resistant mechanism of 56 clinical cases of A baumannii infection from 23 non-tertiary hospitals, collected between 2004 and 2006. METHODS: Susceptibility testing was performed by broth microdilution and Epsilometer test. Analyses of quinolone resistance-determining region (QRDR) were done by sequencing. The activity of the efflux pump was measured using inhibitors. RESULTS: The sequences from selected 56 isolates were divided into seven groups (I-VII) on the basis of mutations in gyrA (S83L), parC (S80L, S80W and S84K) and gyrB (containing the novel mutations E679D, D644Y and A677V). The 27 isolates with triple mutations in gyrA, gyrB and parC (groups IV-VII) showed higher levels of resistance to ciprofloxacin (minimal inhibitory concentration [MIC] of 16-256 µg/mL) than the 26 isolates with double mutations in gyrA and parC (groups II and III, MIC of 8-64 µ g/mL; p < 0.05). Alterations in the efflux pump were observed in four isolates with the parC S80L mutation (group II) or E84K mutation (group VII), but no effect was observed in an isolate with the parC S80 W mutation (group III). CONCLUSION: These results suggest that triple mutations in clinical isolates of A baumannii contribute to the development of high levels of resistance to fluoroquinolones and that mutations in parC S80L or E84K (groups II and VII) may contribute to alterations in efflux pump activity in A baumannii.

20.
Antimicrob Agents Chemother ; 54(11): 4744-9, 2010 Nov.
Article in English | MEDLINE | ID: mdl-20713681

ABSTRACT

We characterized two new streptogramin A resistance genes from quinupristin-dalfopristin-resistant Enterococcus faecium JS79, which was selected from 79 E. faecium isolates lacking known genes encoding streptogramin A acetyltransferase. A 5,650-bp fragment of HindIII-digested plasmid DNA from E. faecium JS79 was cloned and sequenced. The fragment contained two open reading frames carrying resistance genes related to streptogramin A, namely, genes for an acetyltransferase and an ATP efflux pump. The first open reading frame comprised 648 bp encoding 216 amino acids with a predicted left-handed parallel ß-helix domain structure; this new gene was designated vatH. [corrected] The second open reading frame consisted of 1,575 bp encoding 525 amino acids with two predicted ATPase binding cassette transporters comprised of Walker A, Walker B, and LSSG motifs; this gene was designated vgaD. vgaD is located 65 bp upstream from vatH, [corrected] was detected together with vatH [corrected] in 12 of 179 quinupristin-dalfopristin-resistant E. faecium isolates, and was located on the same plasmid. Also, the 5.6-kb HindIII-digested fragment which was observed in JS79 was detected in nine vgaD- and vatH-containing [corrected] E. faecium isolates by Southern hybridization. Therefore, it was expected that these two genes were strongly correlated with each other and that they may be composed of a transposon. Importantly, vgaD is the first identified ABC transporter conferring resistance to streptogramin A in E. faecium. Pulsed-field gel electrophoresis patterns and sequence types of vgaD- and vatH-containing [corrected] E. faecium isolates differed for isolates from humans and nonhumans.


Subject(s)
Enterococcus faecium/drug effects , Streptogramin A/pharmacology , ATP-Binding Cassette Transporters/chemistry , ATP-Binding Cassette Transporters/genetics , Amino Acid Sequence , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Blotting, Southern , DNA Transposable Elements/genetics , Drug Resistance, Bacterial/genetics , Electrophoresis, Gel, Pulsed-Field , Enterococcus faecium/genetics , Enterococcus faecium/metabolism , Genotype , Molecular Sequence Data , Polymerase Chain Reaction , Sequence Homology, Amino Acid
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