Ratiometric measurement of MAM Ca2+ dynamics using a modified CalfluxVTN.

Mitochondria-associated ER membrane (MAM) is a structure where these calcium-regulating organelles form close physical contact sites for efficient Ca2+ crosstalk. Despite the central importance of MAM Ca2+ dynamics in diverse biological processes, directly and specifically measuring Ca2+ concentrations inside MAM is technically challenging. Here, we develop MAM-Calflux, a MAM-specific BRET-based Ca2+ indicator. The successful application of the bimolecular fluorescence complementation (BiFC) concept highlights Ca2+-responsive BRET signals in MAM. The BiFC strategy imparts dual functionality as a Ca2+ indicator and quantitative structural marker specific for MAM. As a ratiometric Ca2+ indicator, MAM-Calflux estimates steady-state MAM Ca2+ levels. Finally, it enables the visualization of uneven intracellular distribution of MAM Ca2+ and the elucidation of abnormally accumulated MAM Ca2+ from the neurons of Parkinson's disease mouse model in both steady-state and stimulated conditions. Therefore, we propose that MAM-Calflux can be a versatile tool for ratiometrically measuring dynamic inter-organellar Ca2+ communication.

A study on smartphone dependence and depression in Korean high school students.

Using the 10th Korean Youth Risk Behavior Web-based Survey in 2020, we attempted to determine the relationship between smartphone addiction and depression in 25,987 high school students. The demographic characteristics of the subjects were reviewed frequently, and the correlation between smartphone dependence and depression were determined. The results show that it is difficult to track the time spent on smartphones to determine the level of depression (R = 0.143, P < .01), it is difficult concentrating on other tasks due to smartphones (R = 0.140, P < .01), and it is difficult to remove images/data received on smartphones from the head (R = 0.141, P < .01). Further, the impulse to use smartphones is strong (R = 0.157, P < .01), health concerns (r = .124, P < .01) and family conflicts exist due to the use of smartphones (r = .149, P < .01), problems with social relations exist due to the use of smartphones (R = 112, P < .01), and difficulties exist in performing tasks due to the use of smartphones (R = 0.153, P < .01). According to the results of the study, it was determined that there is a relationship between smartphone usage and depression among Korean high school students. The findings from this study can be referenced to help guide the development of smartphone usage parameters for Korean teenagers in depression management programs.

Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment.

Regulation of microtubule dynamics is required to properly control various steps of neurodevelopment. In this study, we identified granule cell antiserum-positive 14 (Gcap14) as a microtubule plus-end-tracking protein and as a regulator of microtubule dynamics during neurodevelopment. Gcap14 knockout mice exhibited impaired cortical lamination. Gcap14 deficiency resulted in defective neuronal migration. Moreover, nuclear distribution element nudE-like 1 (Ndel1), an interacting partner of Gcap14, effectively corrected the downregulation of microtubule dynamics and the defects in neuronal migration caused by Gcap14 deficiency. Finally, we found that the Gcap14-Ndel1 complex participates in the functional link between microtubule and actin filament, thereby regulating their crosstalks in the growth cones of cortical neurons. Taken together, we propose that the Gcap14-Ndel1 complex is fundamental for cytoskeletal remodeling during neurodevelopmental processes such as neuronal processes elongation and neuronal migration.

[Determining factors of intention to actual use of charged long-term care services for the aged].

OBJECTIVES: To help develop strategies to cope with the changes arising from the rapid aging process by predicting the determining factors of intention to actual use of the charged long-term care services for elderly as perceived by the middle aged who play the major role of supports. METHODS: Subjects were the parents (men 177, women 507) in their 40s of the students selected from a university of Busan city. A questionnaire survey was conducted for 4 weeks in October 2003 about the knowledge for long-term care service, the intention of actual use, and the preferences about the type of service suppliers. Data analysis was performed with frequency, chi-square test, and t-test using SPSS program (ver 10.0K), along with data mining using decision tree of Enterprise Miner V8.2 by SAS. RESULTS: About half of the subjects (53.7%) had the actual experiences of elderly supports. Intentions to use the charged services were relatively high in home visiting nursing care service (40.1%) and long-term care facilities service (40.4%), and were influenced by previous knowledge about the services. The intentions were stronger in women, those with higher education, and those with greater income levels. Actual elderly supports were mc (80%) done by women, and the perceived burdens for supports were bigger in women and those of lower s economic level. Desired charges were about 10,000 for the bath service, 20,000 won for the rests services day, and about 500,000 won for the long-term care facil service per month. From the result of decision analysis, the job professionalism was the most impol determining factor of intention to actual use of the serv with validation as 63-71%. Health and welfare mixed facilities were preferred, and the most impor consideration was the level of professionalism. CONCLUSIONS: Intention to actual use of the chad services was largely determined by the aspects of time cost. Polices to increase the number of service supp and to decrease the burdens perceived by ac supporters were strongly recommended.