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1.
J Biol Chem ; 273(47): 31581-9, 1998 Nov 20.
Article in English | MEDLINE | ID: mdl-9813074

ABSTRACT

The hepatic CYP4A enzymes are important fatty acid and prostaglandin omega-hydroxylases that are highly inducible by fibric acid hypolipidemic agents and other peroxisome proliferators. Induction of the CYP4A enzymes by peroxisome proliferators is mediated through the nuclear peroxisome proliferator-activated receptor alpha (PPARalpha). Fatty acids have recently been identified as endogenous ligands of PPARalpha, and this receptor has been implicated in the regulation of lipid homeostasis. In the present report we characterized the induction of the hepatic CYP4A genes in rats during the altered lipid metabolism associated with starvation and diabetes. The mRNA levels of CYP4A1, CYP4A2, and CYP4A3 were induced 7-17-fold in the livers of fasted animals and 3-8-fold in the livers of diabetic animals. This was accompanied by corresponding changes in CYP4A protein levels and arachidonic and lauric acid omega-hydroxylase activity. Interestingly, feeding animals after the fasting period caused as much as an 80% suppression of CYP4A mRNA levels, whereas CYP4A protein levels and functional activity returned to control values. A second PPARalpha-responsive gene, acyl-CoA oxidase, was also induced in rat liver by diabetes and fasting. By using PPARalpha-deficient mice, we unambiguously demonstrated that PPARalpha is strictly required for hepatic CYP4A induction by starvation and diabetes. Similarly, induction of hepatic thiolase and bifunctional enzyme also required expression of PPARalpha. This represents the first evidence for the pathophysiologically induced activation of a nuclear receptor.


Subject(s)
Adaptation, Physiological , Cytochrome P-450 Enzyme System/biosynthesis , Diabetes Mellitus, Experimental/metabolism , Isomerases , Liver/enzymology , Mixed Function Oxygenases/biosynthesis , Receptors, Cytoplasmic and Nuclear/metabolism , Starvation/metabolism , Transcription Factors/metabolism , 3-Hydroxyacyl CoA Dehydrogenases/biosynthesis , Acetyl-CoA C-Acetyltransferase/biosynthesis , Animals , Arachidonic Acid/metabolism , Cytochrome P-450 CYP4A , Cytochrome P-450 Enzyme System/genetics , Diabetes Mellitus, Experimental/complications , Enoyl-CoA Hydratase/biosynthesis , Enzyme Induction , Food , Lauric Acids/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Mutant Strains , Microsomes, Liver/enzymology , Mixed Function Oxygenases/genetics , Multienzyme Complexes/biosynthesis , Peroxisomal Bifunctional Enzyme , RNA, Messenger/analysis , Rats , Rats, Sprague-Dawley , Receptors, Cytoplasmic and Nuclear/genetics , Starvation/complications , Streptozocin , Transcription Factors/genetics
2.
Mol Cell Endocrinol ; 139(1-2): 109-15, 1998 Apr 30.
Article in English | MEDLINE | ID: mdl-9705079

ABSTRACT

The mMC5 receptor was cloned from a genomic library, mutated in the extracellular loops (EL's), expressed and tested for binding to melanocyte stimulating hormone (MSH) peptides. The EL's show low amino acid homology within the MC receptor family. Two mutants of the mMC5 receptor were created in order to investigate the participation of these regions in ligand binding. The EL1 and EL3 were separately altered by multiple mutagenesis so that their amino acid sequences became identical with the hMC1 receptor. The mutants were expressed in COS cells and found to bind peptide ligands in the same fashion as the wild type mMC5 receptor clone. The results indicate that the amino acids that were mutated in the mMC5 receptor do not participate in binding of MSH peptides. Comparison of the wild type mMC5 receptor with the hMC5 receptor showed that it has the same potency order for the MSH peptides but considerably higher affinity than the hMC5 receptor.


Subject(s)
Melanocyte-Stimulating Hormones/chemistry , Receptors, Corticotropin/chemistry , Amino Acid Sequence , Animals , COS Cells , Cloning, Molecular , Humans , Kinetics , Ligands , Mice , Molecular Sequence Data , Mutation , Peptides/metabolism , Receptors, Melanocortin , Sequence Homology, Amino Acid
3.
Mol Pharmacol ; 54(1): 154-61, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9658201

ABSTRACT

The melanocortin receptors MC1 and MC3 are G protein-coupled receptors that have substantial structural similarities and bind melanocyte peptides but with different affinity profiles. We constructed a series of chimeric MC1/MC3 receptors to identify the epitopes that determine their selectivities for natural melanocyte peptides and synthetic analogues. The chimeric constructs were made by a polymerase chain reaction that used identical regions in or just outside transmembranes (TM) 1, 4, and 6 and divided the receptors into four segments. Saturation and competition studies on the expressed chimeric proteins indicate that TM1, TM2, TM3, and TM7 are involved in the subtype-specific binding of melanocyte peptides to these receptors. The results support the hypothesis that TM4 and TM5 may not contribute to the ligand-binding specificity of the MC receptors. This is the first report to describe the subtype-specific hormone-binding domains of the melanocortin receptor family.


Subject(s)
Melanocyte-Stimulating Hormones/metabolism , Melanocytes/metabolism , Receptors, Corticotropin/metabolism , Recombinant Fusion Proteins/metabolism , Amino Acid Sequence , Base Sequence , Binding, Competitive , Humans , Molecular Sequence Data , Protein Conformation , Receptor, Melanocortin, Type 3 , Receptors, Corticotropin/chemistry , Receptors, Melanocortin , Sequence Alignment
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