Effects of Input Parameter Range on the Accuracy of Artificial Neural Network Prediction for the Injection Molding Process.

Artificial neural network (ANN) is a representative technique for identifying relationships that contain complex nonlinearities. However, few studies have analyzed the ANN's ability to represent nonlinear or linear relationships between input and output parameters in injection molding. The melt temperature, mold temperature, injection speed, packing pressure, packing time, and cooling time were chosen as input parameters, and the mass, diameter, and height of the injection molded product as output parameters to construct an ANN model and its prediction performance was compared with those of linear regression and second-order polynomial regression. Following the preliminary experiment results, the learning data sets were divided into two groups, i.e., one showed linear relation between the mass of the final product and the range of packing time (linear relation group), and the other showed clear nonlinear relation (nonlinear relation group). The predicted results of ANN were relatively better than those of linear regression and second-order polynomial for both linear and nonlinear relation groups in our specific data sets of the present study.

Innovative Injection Molding Process for the Fabrication of Woven Fabric Reinforced Thermoplastic Composites.

Woven fabric reinforced thermoplastic composites have been gaining significant attention as a lightweight alternative to metal in various industrial fields owing to their high stiffness and strength. Conventional manufacturing processes of woven fabric reinforced thermoplastic composites can be divided into two steps: first, the manufacturing of intermediate material, known as prepreg; then, the formation of the final products from the prepregs. This two-step process increases the manufacturing cost and time of the final composite products. This study demonstrated that woven fabric reinforced thermoplastic composites could be fabricated by an innovative injection molding process instead of the two-step process. A structure placing an extra mesh, which is a new and key component, on the mold-side of woven fabric was devised so that the thermoplastic matrix could be impregnated up to the surface of the woven fabric during injection molding. Tensile tests were performed in the direction parallel to the yarns of the fabric on the injection-molded composites to confirm their mechanical properties. As a result, it was possible to fabricate woven fabric reinforced thermoplastic composites with increased mechanical properties using injection molding without prepreg, and the composites could be molded with a much shorter cycle time than the conventional process, such as thermoforming or over-molding process.

An Experimental Study on Color Shift of Injection-Molded Mobile LGP Depending on Surface Micropattern.

In the display industry, the LCD backlight unit (BLU) module is variously used in mobile phones, notebook computers, monitors, and TVs. The light guide plate (LGP), which is one of the core parts of a BLU, is getting bigger and thinner consistently. Conventional injection methods and injection processes like injection compression molding (ICM) are becoming more complex and harsher with high-speed injection at high mold and melt temperatures. These approaches lead to a change in physical properties and a decrease in optical properties such as yellowing and color shift in injection-molded parts. In the present study, an injection molding experiment was conducted to understand the effect of surface patterns and major injection process conditions like mold and melt temperatures on the color shift in injection-molded mobile LGP. Optical properties obtained by the direct and total transmittance and CIE xy chromaticity diagram for injection-molded mobile LGP were measured with a UV-visible spectrophotometer. From the measurement of patternless LGP, it was found that total or direct transmittance was not affected by molding process variables. It was also found that yellow shift, ΔE(xy), occurred as much as 0.00111 ± 0.00014, and a color shift angle, Θ(xy), of 43.05 ± 2.44° was recorded in CIE coordinates for all nine experimental cases. From the measurement of total transmittance of patterned LGP, ΔE(xy) and Θ(xx) were found to be almost the same as those of patternless LGP for the locations of low and medium density of the pattern for the LGP, T1 and T2. The measured data of direct transmittance of patterned LGP showed that additional yellow shift due to scattering caused by surface micropattern. Interestingly, Θ(xy) of patterned data remained 43.05 ± 2.44°, which was almost the same as that found in the case of patternless LGP.

Computational Fluid Dynamics of Intracranial and Extracranal Arteries using 3-Dimensional Angiography: Technical Considerations with Physician's Point of View.

We investigate the potentials and limitations of computational fluid dynamics (CFD) analysis of patient specific models from 3D angiographies. There are many technical problems in acquisition of proper vascular models, in pre-processing for making 2D surface and 3D volume meshes and also in post-processing steps for display the CFD analysis. We hope that our study could serves as a technical reference to validating other tools and CFD results.

Effects of degumming conditions on electro-spinning rate of regenerated silk.

Electro-spun silk webs are potentially good candidates as tissue engineering scaffolds owing to their good bio- and cyto-compatibility. However, the low fabrication rate of electro-spun silk mats has been one of the obstacles in the mass production of such nanofibrous silk mats in applications to the biomedical field. In this study, the effects of degumming ratio and silk concentration on the electro-spinning process were investigated by using regenerated silk with different residual sericin contents and different silk concentrations in terms of the morphology and structure of the electro-spun silk web. The rate of production of electro-spun silk mats could be increased by approximately 5 fold at a degumming ratio of 19.5%. The electro-spinning rate of silk was affected by two main factors: (1) dope solution viscosity and (2) degumming ratio of silk. The conductivity of the silk dope solution, however, had little effects on the electro-spinning of regenerated silk. A constant spun fiber morphology was observed within the electro-spinning rate range (0.3-1.4 ml/h). Fourier transform infrared spectroscopy showed that partial ß-sheet crystallization occurred during electro-spinning. The molecular conformation was relatively unaffected by the electro-spinning rate of silk.

Computational modeling with fluid-structure interaction of the severe m1 stenosis before and after stenting.

PURPOSE: Image-based computational models with fluid-structure interaction (FSI) can be used to perform plaque mechanical analysis in intracranial artery stenosis. We described a process in FSI study applied to symptomatic severe intracranial (M1) stenosis before and after stenting. MATERIALS AND METHODS: Reconstructed 3D angiography in STL format was transferred to Magics for smoothing of vessel surface and trimming of branch vessels and to HyperMesh for generating tetra volume mesh from triangular surface-meshed 3D angiogram. Computational analysis of blood flow in the blood vessels was performed using the commercial finite element software ADINA Ver 8.5. The distribution of wall shear stress (WSS), peak velocity and pressure was analyzed before and after intracranial stenting. RESULTS: The wall shear stress distributions from Computational fluid dynamics (CFD) simulation with rigid wall assumption as well as FSI simulation before and after stenting could be compared. The difference of WSS between rigid wall and compliant wall model both in pre- and post-stent case is only minor except at the stenosis region. These WSS values were greatly reduced after stenting to 15~20 Pa at systole and 3~5 Pa at end-diastole in CFD simulation, which are similar in FSI simulations. CONCLUSION: Our study revealed that FSI simulation before and after intracranial stenting was feasible despite of limited vessel wall dimension and could reveal change of WSS as well as flow velocity and wall pressure.

Effect of degumming condition on the solution properties and electrospinnablity of regenerated silk solution.

The application of silk on tissue engineering scaffolds has been studied intensively because silk has an electrospinning technique using a good blood compatibility, excellent cytocompatibility and biodegradability. Silk consists of two polymers, fibroin and sericin. In spite of importance of sericin, most studies were focused on the fibroin only and the effect of residual sericin on the electrospinning performance of silk has not been considered. In this study, regenerated silk with different residual sericin contents was prepared by controlling the degumming conditions. The effects of the degumming conditions on the solution properties and electrospinning performance of silk were examined. The fast protein liquid chromatography (FPLC) measurements confirmed that the molecular weight of the regenerated silk decreased slightly with increasing residual sericin content. More molecular aggregation of silk occurred with increasing sericin content, resulting in an increase in the solution turbidity of formic acid. All silk formic acid solutions exhibited almost Newtonian fluid behavior and the viscosity increased with increasing sericin content. Interestingly, the dope solution viscosity of silk increased remarkably at sericin contents <1% (or degumming ratio >25%) leading to significant improvements in electrospinnability and an increase in the fiber diameter of the silk web.

Computational flow dynamics of the severe m1 stenosis before and after stenting.

PURPOSE: Computational flow dynamic (CFD) study has not been widely applied in intracranial artery stenosis due to requirement of high resolution in identifying the small intracranial artery. We described a process in CFD study applied to symptomatic severe intracranial (M1) stenosis before and after stenting. MATERIALS AND METHODS: Reconstructed 3D angiography in STL format was transferred to Magics (Materialise NV, Leuven, Belgium) for smoothing of vessel surface and trimming of branch vessels and to HyperMesh (Altair Engineering Inc., Auckland, New Zealand) for generating tetra volume mesh from triangular surface-meshed 3D angiogram. Computational analysis of blood flow in the blood vessels was performed using the commercial finite element software ADINA Ver 8.5 (ADINA R & D, Inc., Lebanon, MA). The distribution of wall shear stress (WSS), peak velocity and pressure in a patient was analyzed before and after intracranial stenting. RESULTS: Computer simulation of wall shear stress, flow velocity and wall pressure before and after stenting could be demonstrated three dimensionally by video mode according to flow vs. time dimension. Such flow model was well correlated with angiographic finding related to maximum degree of stenosis. Change of WSS, peak velocity and pressure at the severe stenosis was demonstrated before and after stenting. There was no WSS after stenting in case without residual stenosis. CONCLUSION: Our study revealed that CFD analysis before and after intracranial stenting was feasible despite of limited vessel wall dimension and could reveal change of WSS as well as flow velocity and wall pressure.

High shear stress at the surface of enhancing plaque in the systolic phase is related to the symptom presentation of severe M1 stenosis.

The computational fluid dynamics methods for the limited flow rate and the small dimensions of an intracranial artery stenosis may help demonstrate the stroke mechanism in intracranial atherosclerosis. We have modeled the high wall shear stress (WSS) in a severe M1 stenosis. The high WSS in the systolic phase of the cardiac cycle was well-correlated with a thick fibrous cap atheroma with enhancement, as was determined using high-resolution plaque imaging techniques in a severe stenosis of the middle cerebral artery.

High flux filtration medium based on nanofibrous substrate with hydrophilic nanocomposite coating.

A novel high flux filtration medium, consisting of a three-tier composite structure, i.e., a nonporous hydrophilic nanocomposite coating top layer, an electrospun nanofibrous substrate midlayer, and a conventional nonwoven microfibrous support, was demonstrated for oil/water emulsion separations for the first time. The nanofibrous substrate was prepared by electrospinning of poly(vinyl alcohol) (PVA) followed by chemical cross-linking with glutaraldehyde (GA) in acetone. The resulting cross-linked PVA substrates showed excellent water resistance and good mechanical properties. The top coating was based on a nanocomposite layer containing hydrophilic polyether-b-polyamide copolymer or a cross-linked PVA hydrogel incorporated with surface-oxidized multiwalled carbon nanotubes (MWNTs). Scanning electron microscopy (SEM) examinations indicated that the nanocomposite layer was nonporous within the instrumental resolution and MWNTs were well dispersed in the polymer matrix. Oil/ water emulsion tests showed that this unique type of filtration media exhibited a high flux rate (up to 330 L/m2-h at the feed pressure of 100 psi) and an excellent total organic solute rejection rate (99.8%) without appreciable fouling. The increase in the concentration of surface-oxidized MWNT in the coating layer generally improves the flux rate, which can be attributed to the generation of more effective hydrophilic nanochannels for water passage in the composite membranes.

A rapid determination of propiverine and its N-oxide metabolite in human plasma by high performance liquid chromatography-electrospray ionization tandem mass spectrometry.

A simple, fast and sensitive high-performance liquid chromatography (HPLC)-electrospray ionization (ESI) tandem mass spectrometric method (LC-MS/MS) has been developed for determination of propiverine and propiverine N-oxide metabolite in human plasma using oxybutynin as internal standard. Instead of extracting propiverine from plasma using organic solvents, which should be separated from the aqueous phase and evaporated before injecting the sample into the chromatograph, plasma sample containing propiverine and N-oxide was directly injected after precipitating proteins with acetonitrile. Numerous compounds in the plasma did not interfere with the highly specific multiple reaction monitoring in tandem mass spectrometric detection following C(8) reversed-phase chromatographic separation under conditions that eluted propiverine, N-oxide and oxybutynin within 2min (0.1% formic acid in water/acetonitrile, 25:75, v/v). The LC-MS/MS method and an alternative LC-MS method, using methyl-t-butyl ether extraction and selected ion monitoring, were validated over 1-250ngml(-1) of propiverine and 2 to 500ngml(-1) of N-oxide, and successfully applied in a pharmacokinetic study. The lower limit of quantitation was 1ngml(-1) for propiverine and 2ngml(-1) for N-oxide in both methods.

Simultaneous determination of amoxicillin and clavulanic acid in human plasma by HPLC-ESI mass spectrometry.

A simple, fast and sensitive high-performance liquid chromatography (HPLC)-mass spectrometric (MS) method has been developed for simultaneous determination of amoxicillin and clavulanic acid in human plasma using terbutaline as internal standard. After precipitation of the plasma proteins with acetonitrile, the analytes were separated on a C(8) reversed-phase column with formic acid-water-acetonirile (2:1000:100) and detected using electrospray ionization (ESI) mass spectrometry in negative selected ion monitoring (SIM) mode. The method was validated and successfully applied to analysis of amoxicillin and clavulanic acid in clinical studies. The limit of quantitation, 0.12 microg/ml for amoxicillin and 0.062 microg/ml for clavulanic acid, was five times lower than that of the published HPLC-UV method.

DNA capillary electrophoresis using poly(vinyl alcohol). I. Inner capillary coating.

Two new methods of inner capillary coating with poly(vinyl alcohol) (PVAL) have been investigated and evaluated by performing DNA capillary electrophoresis (CE) using PVAL as a separation medium and by measuring the electroosmotic flow (EOF) mobility. The treatment of capillaries with a silanol-group modified PVAL (PVAL-Si) has been found to give good coating effects for improving the resolution of DNA CE and for reducing the EOF. This coating must be effectively achieved by combining the adsorptive property of PVAL chains onto silica with the reaction between the silanol groups of PVAL-Si and the silica surface. The adsorption of PVAL onto silica has been observed by using atomic force microscopy (AFM) for PVAL-Si as well as for a nonmodified PVAL as a control. The coating with PVAL that links to the capillary wall surface with more hydrolytically stable bonding, -Si-C-, has been formed by performing the Grignard reaction, followed by in-capillary polymerization of vinyl acetate (VAc) and hydrolysis. This coating has been found to be effective for improving the resolution of DNA CE and for reducing the EOF.

DNA capillary electrophoresis using poly(vinyl alcohol). II. Separation media.

We represent the first extensive study on DNA capillary electrophoresis using various poly(vinyl alcohol) (PVAL)-related polymers as separation media. As a separation medium, PVAL homopolymer has shown poor resolutions probably due to its very strong hydrogen-bonding characteristics, resulting in extensive self-aggregation. On the other hand, poly(vinyl alcohol-co-vinyl acetate) and poly(vinyl alcohol-co-1-vinyl-2-pyrrolidone) (PVAL-VP), both with a degree of polymerization of approximately 3.0x10(3), have been found to give excellent electropherograms with good resolutions for the analysis of double-stranded (ds)DNA. PVAL-VP, with hydrolytic stability in high and low pH regions, has also yielded fair electropherograms for single-stranded (ss)DNA under neutral and alkaline conditions, although further investigation is essential in order to increase the resolutions necessary for DNA sequencing analysis. The separations obtained under alkaline conditions have shown significantly shorter retention times, one-third of that for the current commercial separation media, due to the higher ionization of phosphate groups in the DNA chains.