Seminal profile of 23,504 patients over the years: 7 years of experience.

OBJECTIVE: To evaluate the behavior of seminal parameters over the years - 2010 to May 2017. METHODS: A retrospective study, carried out from January 2010 to May 2017, covering men who underwent sperm examination. Seminal parameters (volume, sperm concentration, motility, morphology, age and duration of infertility in years) of 23,504 men were evaluated. The groups were compared to each other to check whether there were changes to the seminal parameters in that period. RESULTS: There was no change over the years considering the time of infertility, in an average of 3.78 years. The mean age of the patients was 36.53 years, with a trend of increase in 0.2 years. In relation to the volume of semen samples, the mean value was 3.29mL, with a decrease trend in 0.05mL. As for sperm concentration, the average was 34.37 million/mL, with a decrease trend of 1.0 million/mL. Progressive sperm motility showed an average of 47.27% and there was a decrease trend of 0.67%. Finally, sperm morphology presented an average of 2.79% of normal spermatozoa, with a decrease trend of 0.33%. CONCLUSION: It can be noted that over the years, the sperm quality of men looking for assisted reproduction clinics has tended to decrease in macroscopic and microscopic parameters (volume, sperm concentration, motility and morphology).

Next Generation Sequencing (NGS) in chromosome translocation 46, XX, t (9; X) (q22; q28) - a case report.

This paper reports the case of a patient who sought assisted reproductive technology (ART) treatment and was referred to pre-implantation genetic diagnosis (PGD) on account of a chromosomal translocation presented with secondary infertility. The patient underwent a highly complex ART treatment and had 14 metaphase II oocytes collected on the day of follicular aspiration. The embryos were taken to extended culture and five were biopsied and vitrified. The embryo genetic report showed aneuploidy in four of the blastocysts, while the other resulted in 46, XX. In conclusion, chromosome translocations involving the X chromosome might result in the deregulation of gene expression and defective ovarian formation. Therefore, the genes present in the X chromosome are believed to be essential in normal ovarian function.