1.
Biosci Biotechnol Biochem
; 77(12): 2480-2, 2013.
Article
in English
| MEDLINE
| ID: mdl-24317047
ABSTRACT
We describe a transient dual-luciferase assay combined with a glucocorticoid-inducible system for rice protoplasts. Luciferase genes were efficiently induced by adding 0.1 µM of dexamethasone to the protoplast suspension, the activity of the luciferases reaching a maximum 6 h after induction. This assay system is applicable to studying the translation efficiency of rice by using the luciferase gene harboring tandem copies of an interesting codon at the 5' end.