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1.
Yakugaku Zasshi ; 144(4): 463-471, 2024.
Article in Japanese | MEDLINE | ID: mdl-38556319

ABSTRACT

In Japan, the use of frame retardants [tris(2,3-dibromopropyl)phosphate: TDBPP and bis(2,3-dibromopropyl)phosphate: BDBPP] in several household textile products is banned under the "Act on the Control of Household Products Containing Harmful Substances." As the official analytical methods for testing these substances have not been revised for over 42 years, several issues such as the using of harmful reagents, have been pointed out. Therefore, we developed a new method to revise the official method in our previous study. In this study, the validity of the developed test method is evaluated at six laboratories using two types of textile samples spiked with TDBPP and BDBPP at three concentrations (4, 8, and 20 µg/g). TDBPP and BDBPP are extracted under reflux using methanol containing hydrochloric acid. TDBPP is analyzed using GC-MS, and BDBPP is also analyzed using GC-MS after methylation with trimethylsilyl diazomethane. Although the accuracy (70-120%), repeatability (<10%), and reproducibility (<15%) of a few samples, mainly low concentration samples, are out of range, overall, the concentration level of detection limits of TDBPP and BDBPP (8 and 10 µg/g) in official analytical methods are quantifiable with sufficient precision using the proposed method. Furthermore, harmful reagents are not used in this method. Thus, the method validated in this study is effective as a revised method for the testing of TDBPP and BDBPP in household textile products.


Subject(s)
Organophosphates , Phosphates , Reproducibility of Results , Household Products
2.
J Chromatogr A ; 1698: 464007, 2023 Jun 07.
Article in English | MEDLINE | ID: mdl-37099903

ABSTRACT

Polycyclic aromatic hydrocarbons (PAHs) in creosote products used for wood preservation are regulated in Japan. Although the analytical method for this regulation has been stipulated by law, two main problems have been highlighted, namely the use of dichloromethane, a potential carcinogen, as a solvent and inadequate purification. Therefore, an analytical method to solve these problems was developed in this study. Actual creosote-treated wood samples were examined, and it was found that acetone could be used as an alternative solvent. Purification methods using centrifugation, silica gel cartridges, and strong anion exchange (SAX) cartridges were also developed. It was found that the SAX cartridges strongly retained PAHs, and an effective purification method was developed using this phenomenon, in which contaminants were removed by washing with diethyl ether/hexane (1/9 v/v), which could not be achieved with a silica gel cartridge. This strong retention was attributed to cation-π interactions. The analytical method developed in this study yielded good recoveries (81.4-113.0%) with low relative standard deviations (<6.8%), and the limit of quantification (0.02-0.29 µg/g) was significantly lower than the current creosote product regulation. Therefore, this method can safely and effectively extract and purify PAHs from creosote products.


Subject(s)
Creosote , Polycyclic Aromatic Hydrocarbons , Silica Gel , Wood , Solvents
3.
Chem Pharm Bull (Tokyo) ; 69(8): 741-746, 2021 Aug 01.
Article in English | MEDLINE | ID: mdl-34024880

ABSTRACT

Citrus-type crude drugs (CCDs) are commonly used to formulate decoctions in Kampo formula (traditional Japanese medicine). Our previous study reported metabolomic analyses for differentiation of the methanol extracts of Citrus-type crude drugs (CCDs) using ultra-HPLC (UHPLC)/MS, and 13C- and 1H-NMR. The present study expanded the scope of its application by analyzing four CCD water extracts (Kijitsu, Tohi, Chimpi, and Kippi); these CCDs are usually used as decoction ingredients in the Kampo formula. A principal component analysis score plot of processed UPLC/MS and NMR analysis data indicated that the CCD water extracts could be classified into three groups. The loading plots showed that naringin and neohesperidin were the distinguishing components. Three primary metabolites, α-glucose, ß-glucose, and sucrose were identified as distinguishing compounds by NMR spectroscopy. During the preparation of CCD dry extracts, some compounds volatilized or decomposed. Consequently, fewer compounds were detected than in our previous studies using methanol extract. However, these results suggested that the combined NMR- and LC/MS-based metabolomics can discriminate crude drugs in dried water extracts of CCDs.


Subject(s)
Citrus/chemistry , Fruit and Vegetable Juices/analysis , Plant Extracts/analysis , Chromatography, High Pressure Liquid , Flavanones/chemistry , Glucose/chemistry , Hesperidin/analogs & derivatives , Hesperidin/chemistry , Magnetic Resonance Spectroscopy , Metabolomics , Methanol/chemistry , Principal Component Analysis , Sucrose/chemistry , Tandem Mass Spectrometry , Water
4.
J Nat Med ; 75(1): 11-27, 2021 Jan.
Article in English | MEDLINE | ID: mdl-32740706

ABSTRACT

Saposhnikoviae Radix (SR), derived from the dried root and rhizome of Saposhnikovia divaricata, is a popular crude drug used in traditional Chinese and Japanese medicine. To evaluate the metabolites of S. divaricata roots from Mongolia and to investigate their geographical variation, we developed the HPLC method, determined the contents of 9 chromones and 4 coumarins, and conducted multivariate statistical analysis. All Mongolian specimens contained prim-O-glucosylcimifugin (1) and 4'-O-ß-D-glucosyl-5-O-methylvisamminol (3), and their total amount (5.04-25.06 mg/g) exceeded the criterion assigned in the Chinese Pharmacopoeia. Moreover, the content of 1 (3.98-20.79 mg/g) was significantly higher in the Mongolian specimens than in Chinese SR samples. The specimens from Norovlin showed the highest contents of 1 and 3. The total levels of dihydropyranochromones were higher in the specimens from Bayan-Uul. The orthogonal partial least squares-discriminant analysis revealed that the Mongolian specimens tended to be separated into three groups based on growing regions, in which several chromones contributed to each distribution. Furthermore, 1H NMR analysis revealed that Mongolian specimens had less amount of sucrose and a substantial amount of polyacetylenes. Thus, in this study, the chemical characteristics of Mongolian S. divaricata specimens were clarified and it was found that the specimens from the northeast part of Mongolia, including Norovlin, had the superior properties due to higher amounts of major chromones.


Subject(s)
Apiaceae/chemistry , Plant Roots/chemistry , Mongolia
5.
J Nat Med ; 74(3): 550-560, 2020 Jun.
Article in English | MEDLINE | ID: mdl-32333306

ABSTRACT

Previously, we established a 1H NMR metabolomics method using reversed-phase solid-phase extraction column (RP-SPEC), and succeeded in distinguishing wild from cultivated samples of Saposhnikoviae radix (SR), and between SR and its substitute, Peucedanum ledebourielloides root (PR). Herein, we performed LC-HR/MS metabolomics using fractions obtained via RP-SPEC to identify characteristic components of SR and PR. One and three characteristic components were respectively found for SR and PR; these components were isolated with their m/z values and retention times as a guide. The characteristic component of SR was identified as 4'-O-ß-D-glucosyl-5-O-methylvisamminol (1), an indicator component used to identify SR in the Japanese Pharmacopoeia. In contrast, the characteristic components of PR were identified as xanthalin (2), 4'-O-ß-D-apiosyl (1 → 6)-ß-D-glucosyl-5-O-methylvisamminol (3), and 3'-O-ß-D-apiosyl (1 → 6)-ß-D-glucosylhamaudol (4) based on spectroscopic data such as 1D- and 2D-NMR, MS, and specific optical rotation. Among them, 4 is a novel compound. For the correlation between the NMR metabolomics results in the present and our previous report, only 1 and 2 were found to correlate with the chemical shifts, and the other compounds had no correlation. As the chemical shifts for compounds 1, 3, and 4 were similar to each other, especially for the aglycone moiety, they could not be distinguished because of the sensitivity and resolution of 1H NMR. Accordingly, combining NMR and LC/MS metabolomics with their different advantages is considered useful for metabolomics of natural products. The series of methods used in our reports could aid in quality evaluations of natural products and surveying of marker components.


Subject(s)
Apiaceae/chemistry , Apiaceae/classification , Drugs, Chinese Herbal/chemistry , Plant Extracts/chemistry , Plant Roots/chemistry , Chromatography, High Pressure Liquid , Coumarins , Magnetic Resonance Spectroscopy , Mass Spectrometry , Metabolomics/methods , Solid Phase Extraction
6.
J Nat Med ; 74(1): 65-75, 2020 Jan.
Article in English | MEDLINE | ID: mdl-31342251

ABSTRACT

1H NMR-based metabolomics has been applied in research on food, herbal medicine, and natural products. Although excellent results were reported, samples were directly extracted with a deuterated solvent (e.g., methanol-d4 or D2O) in most reports. As primary metabolites account for most of the results, data for secondary metabolites are partially reflected. Consequently, secondary metabolites tend to be excluded from factor loading analysis, serving as a significant unfavorable feature of 1H NMR-based metabolomics when investigating biologically active or functional components in natural products and health foods. Reversed-phase solid-phase extraction column (RP-SPEC) was applied for sample preparation in 1H NMR-based metabolomics to overcome this feature. The methanol extract from Saposhnikoviae radix (SR), an important crude drug, was fractionated with RP-SPEC into 5% methanol-eluting fractions, and the remaining fraction was collected. Each fraction was subjected to 1H NMR-based metabolomics and compared to results from conventional 1H NMR-based metabolomics. Based on principal component analysis (PCA) and partial least squares projections to latent structures discriminant analysis (PLS-DA), the 5% methanol fraction and conventional method reflected the amount of saccharides such as sucrose on the PC1/PLS1 axes, and wild and cultivated samples were discriminated along those axes. The remaining fraction clearly distinguished SR from Peucedanum ledebourielloides root. The compounds responsible for this discrimination were deemed falcarindiol derivatives and other unidentified secondary metabolites from the s-plot on PLS-DA. The secondary metabolites from original plants were, therefore, presumed to be concentrated in the remaining fraction by RP-SPEC treatment and strongly reflected the species differences. The developed series is considered effective to perform quality evaluation of crude drugs and natural products.


Subject(s)
Apiaceae/chemistry , Complex Mixtures/chemistry , Magnetic Resonance Spectroscopy/methods , Metabolomics/methods , Plant Roots/chemistry , Solid Phase Extraction/methods , Proton Magnetic Resonance Spectroscopy
7.
J Nat Prod ; 82(8): 2116-2123, 2019 08 23.
Article in English | MEDLINE | ID: mdl-31322883

ABSTRACT

Five Citrus-type crude drugs (40 samples) were classified using liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. The following six flavonoid derivatives were identified as contributors from the loading plots of multivariate analysis: naringin (1), neohesperidin (2), neoeriocitrin (3), narirutin (9), hesperidin (10), and 3,5,6,7,8,3',4'-heptamethoxyflavone (12). Three coumarin derivatives, namely, meranzin (6), meranzin hydrate (7), and meranzin glucoside (8), were also identified as contributors. Furthermore, compared with our previous studies on proton (1H) and 13C NMR spectroscopy-based metabolomics, the present study revealed that the Citrus-type crude drugs were distinguished with the same pattern; however, the contributors differed between the 1H and 13C NMR spectroscopy-based metabolomics. The high dynamic range of NMR spectroscopy provided broad coverage of the metabolomes including the primary and secondary metabolites. However, LC-MS appeared to be superior in detecting secondary metabolites with high sensitivity, some of which occurred in quantities that were undetectable using NMR spectroscopy.


Subject(s)
Chromatography, High Pressure Liquid/methods , Citrus/chemistry , Magnetic Resonance Spectroscopy/methods , Mass Spectrometry/methods , Metabolomics , Plant Extracts/pharmacology
8.
J Pharm Biomed Anal ; 161: 305-312, 2018 Nov 30.
Article in English | MEDLINE | ID: mdl-30193199

ABSTRACT

Five Citrus-type crude drugs (40 samples) were classified using 13C-NMR spectra-based metabolomics. The following eight metabolites were identified from the loading plots of multivariate analysis of the 13C-NMR spectra; naringin, neohesperidin, narirutin, synephrine, sucrose, α-glucose, ß-glucose, and limonene. 13C-NMR spectra-based metabolic fingerprinting is a promising strategy for classifying crude drugs.


Subject(s)
Carbon-13 Magnetic Resonance Spectroscopy/methods , Citrus/chemistry , Phytochemicals/chemistry , Phytochemicals/metabolism , Molecular Structure
9.
J Nat Med ; 72(1): 267-273, 2018 Jan.
Article in English | MEDLINE | ID: mdl-29149424

ABSTRACT

Saposhnikoviae radix (SR) is described in the Japanese Pharmacopoeia as a crude drug derived from the root of Saposhnikovia divaricata Schischkin (Umbelliferae). According to Flora of China, the root of Peucedanum ledebourielloides K. F. Fu is used as a regional substitute for SR. Therefore, we surveyed the botanical origin of the drug used in China, especially Shaanxi and the surrounding regions, through nucleotide sequence analysis of the internal transcribed spacer region of rDNA. As a result, several samples from Shaanxi () and Shanxi () provinces were identified as Peucedanum ledebourielloides. To prevent this substitute from being distributed as genuine SR, we developed a thin-layer chromatography analysis condition to enable a specific compound of this species to be easily detected. The specific compound was identified as xanthalin, based on 1D- and 2D-NMR and high-resolution mass spectrometry data. The established TLC conditions were as follows-extraction solvent, n-hexane; applied volume, 5 µL; chromatographic support, silica gel; developing solvent, n-hexane:ethyl acetate:acetic acid (20:10:1); developing length, 7 cm; detection, UV (365 nm); R f value, 0.4 (blue fluorescence; xanthalin).


Subject(s)
Drugs, Chinese Herbal/chemistry , Plant Roots/chemistry , China
10.
J Agric Food Chem ; 65(17): 3581-3588, 2017 May 03.
Article in English | MEDLINE | ID: mdl-28398734

ABSTRACT

To construct a model formula to evaluate the thermogenetic effect of ginger (Zingiber officinale Roscoe) from the ingredient information, we established transient receptor potential vanilloid subtype 1 (TRPV1)-stimulating activity prediction models by using a partial least-squares projections to latent structures (PLS) regression analysis in which the ingredient data from liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and the stimulating activity values for TRPV1 receptor were used as explanatory and objective variables, respectively. By optimizing the peak extraction condition of the LC-HRMS data and the data preprocessing parameters of the PLS regression analysis, we succeeded in the construction of a TRPV1-stimulating activity prediction model with high precision ability. We then searched for the components responsible for the TRPV1-stimulating activity by analyzing the loading plot and s-plot of the model, and we identified [6]-gingerol (1) and hexahydrocurcumin (3) as TRPV1-stimulating activity components.


Subject(s)
Plant Extracts/pharmacology , TRPV Cation Channels/analysis , Zingiber officinale/chemistry , Chromatography, High Pressure Liquid , Food Handling , HEK293 Cells , Humans , Least-Squares Analysis , Mass Spectrometry , TRPV Cation Channels/metabolism
11.
Bioorg Med Chem ; 21(22): 6915-9, 2013 Nov 15.
Article in English | MEDLINE | ID: mdl-24095012

ABSTRACT

Pheophytinization of chlorophyll (Chl) c1, which was isolated from the diatom Chaetoceros gracilis, was kinetically analyzed under weakly acidic conditions, and was compared with that of protochlorophyllide (PChlide) a and chlorophyllide (Chlide) a. Chl c1 possessing a trans-acrylic acid residue at the 17-position exhibited slower pheophytinization kinetics than PChlide a and Chlide a, both of which possessed a propionic acid residue at the same position. The difference in pheophytinization properties between Chl c1 and (P)Chlide a was ascribable to the electronegativity of the 17-substituent in Chl c1 larger than that of (P)Chlide a due to the C17(1)-C17(2) double bond with the conjugated 17(2)-carboxy group in Chl c1. Demetalation kinetics of PChlide a was slower than that of Chlide a, which originated from the effect of the π-macrocyclic structures.


Subject(s)
Acrylates/chemistry , Chlorophyll/chemistry , Chlorophyll/metabolism , Chlorophyllides/chemistry , Diatoms/chemistry , Diatoms/metabolism , Isomerism , Kinetics , Protochlorophyllide/chemistry
12.
Bioorg Med Chem ; 21(13): 3689-94, 2013 Jul 01.
Article in English | MEDLINE | ID: mdl-23669190

ABSTRACT

Green photosynthetic bacteria have unique light-harvesting antenna systems called chlorosomes. Chlorobaculum tepidum, a model organism of the bacteria, biosynthesized monogalactosyl- and rhamnosylgalactosyldiacylglycerides possessing a methylene-bridged palmitoleyl group characterized by a cis-substituted cyclopropane ring as the dominant glycolipids of its chlorosome surface. The formation of the cyclopropane ring was chemically inhibited by supplementation of sinefungin, an analog of S-adenosyl-L-methionine, into the bacterial cultivation. The presence of the cyclopropane ring reinforced acid resistance of the light-harvesting chlorosomes and suppressed acidic demetalation (pheophytinization) of bacteriochlorophyll-c pigments constructing the core part of chlorosomes. The ring-formation would represent direct and post-synthetic modifications of chlorosome membrane properties and was tolerant of acidic environments.


Subject(s)
Bacterial Proteins/metabolism , Bacteriochlorophylls/metabolism , Chlorobi/cytology , Chlorobi/metabolism , Cyclopropanes/metabolism , Fatty Acids/metabolism , Glycolipids/metabolism , Acylation , Bacterial Proteins/chemistry , Bacteriochlorophylls/chemistry , Chlorobi/chemistry , Cyclopropanes/chemistry , Fatty Acids/chemistry , Glycolipids/chemistry
13.
Sci Rep ; 3: 1217, 2013.
Article in English | MEDLINE | ID: mdl-23386973

ABSTRACT

Chlorophyllous pigments are essential for photosynthesis. Bacteriochlorophyll (BChl) b has the characteristic C8-ethylidene group and therefore is the sole naturally occurring pigment having an absorption maximum at near-infrared light wavelength. Here we report that chlorophyllide a oxidoreductase (COR), a nitrogenase-like enzyme, showed distinct substrate recognition and catalytic reaction between BChl a- and b-producing proteobacteria. COR from BChl b-producing Blastochloris viridis synthesized the C8-ethylidene group from 8-vinyl-chlorophyllide a. In contrast, despite the highly conserved primary structures, COR from BChl a-producing Rhodobacter capsulatus catalyzes the C8-vinyl reduction as well as the previously known reaction of the C7 = C8 double bond reduction on 8-vinyl-chlorophyllide a. The present data indicate that the plasticity of the nitrogenase-like enzyme caused the branched pathways of BChls a and b biosynthesis, ultimately leading to ecologically different niches of BChl a- and b-based photosynthesis differentiated by more than 150 nm wavelength.


Subject(s)
Bacteriochlorophylls/metabolism , Biosynthetic Pathways , Alphaproteobacteria/enzymology , Alphaproteobacteria/metabolism , Bacteriochlorophyll A/chemistry , Bacteriochlorophyll A/metabolism , Bacteriochlorophylls/chemistry , Oxidoreductases Acting on CH-CH Group Donors/chemistry , Oxidoreductases Acting on CH-CH Group Donors/genetics , Oxidoreductases Acting on CH-CH Group Donors/metabolism , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Rhodobacter capsulatus/enzymology , Rhodobacter capsulatus/metabolism , Spectroscopy, Near-Infrared , Substrate Specificity
14.
Photosynth Res ; 114(3): 179-88, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23420454

ABSTRACT

The compositions of glycolipids in the following seven strains of green photosynthetic bacteria were investigated at the molecular level using LC-MS coupled with an evaporative light scattering detector: Chlorobium (Chl.) limicola strains Larsen (30 °C as the optimal cultivation temperature) and DSM245 (30 °C), Chlorobaculum (Cba.) tepidum strain ATCC49652 (45 °C), Cba. parvum strain NCIB8327 (30 °C), Cba. limnaeum strain 1549 (30 °C), Chl. phaeovibrioides DSM269 (30 °C), and Chloroflexus (Cfl.) aurantiacus strain J-10-fl (55 °C). Dependence of the molecular structures of glycolipids including the chain-length of their acyl groups upon bacterial cultivation temperatures was clearly observed. The organisms with their optimal temperatures of 30, 45, and 55 °C dominantly accumulated glycolipids possessing the acyl chains in the range of C(15)-C(16), C(16)-C(17), and C(18)-C(20), respectively. Cba. tepidum with an optimal temperature of 45 °C preferred the insertion of a methylene group to produce finally a C(17)-cyclopropane chain. Cfl. aurantiacus cultured optimally at 55 °C caused a drastic increase in the chain-length. Notably, the length of such acyl groups corresponded to that of the esterifying chain in the 17-propionate residues of self-aggregative bacteriochlorophylls-c/d/e, indicating stabilization of their supramolecular structures through hydrophobic interactions among those hydrocarbon chains. Based on the detailed compositions of glycolipids, a survival strategy of green photosynthetic bacteria grown in the wide range of temperatures is discussed.


Subject(s)
Chlorobium/chemistry , Chloroflexus/chemistry , Glycolipids/chemistry , Chlorobium/physiology , Chloroflexus/physiology , Gas Chromatography-Mass Spectrometry , Glycolipids/physiology , Molecular Structure , Temperature
15.
Biochim Biophys Acta ; 1807(11): 1467-73, 2011 Nov.
Article in English | MEDLINE | ID: mdl-21806961

ABSTRACT

Chlorophyll(Chl)-c pigments in algae, diatoms and some prokaryotes are characterized by the fully conjugated porphyrin π-system as well as the acrylate residue at the 17-position. The precise structural characterization of Chl-c(3) from the haptophyte Emiliania huxleyi was performed. The conformations of the π-conjugated peripheral substituents, the 3-/8-vinyl, 7-methoxycarbonyl and 17-acrylate moieties were evaluated, in a solution, using nuclear Overhauser enhancement correlations and molecular modeling calculations. The rotation of the 17-acrylate residue was considerably restricted, whereas the other three substituents readily rotated at ambient temperature. Moreover, the stereochemistry at the 13²-position was determined by combination of chiral high-performance liquid chromatography (HPLC) with circular dichroism (CD) spectroscopy. Compared with the CD spectra of the structurally related, synthetic (13²R)- and (13²S)-protochlorophyllide(PChlide)-a, naturally occurring Chl-c3 had exclusively the (13²R)-configuration. To elucidate this natural selection of a single enantiomer, we analyzed the three major Chl-c pigments (Chl-c1, c2 and c3) in four phylogenetically distinct classes of Chl-c containing algae, i.e., heterokontophyta, dinophyta, cryptophyta and haptophyta using chiral HPLC. All the photosynthetic organisms contained only the (13²R)-enantiomerically pure Chls-c, and lacked the corresponding enantiomeric (13²S)-forms. Additionally, Chl-c2 was found in all the organisms as the common Chl-c. These results throw a light on the biosynthesis as well as photosynthetic function of Chl-c pigments: Chl-c2 is derived from 8-vinyl-PChlide-a by dehydrogenation of the 17-propionate to acrylate residues as generally proposed, and the (13²R)-enantiomers of Chls-c function as photosynthetically active, light-harvesting pigments together with the principal Chl-a and carotenoids.


Subject(s)
Chlorophyll/chemistry , Haptophyta/chemistry , Chlorophyll/metabolism , Molecular Structure , Stereoisomerism
16.
Biochemistry ; 50(21): 4504-12, 2011 May 31.
Article in English | MEDLINE | ID: mdl-21510667

ABSTRACT

The green sulfur photosynthetic bacterium Chlorobaculum (Cba.) tepidum (previously known as Chlorobium tepidum), which grows at an optimal temperature of around 45 °C, biosynthesized unique disaccharide rhamnosylgalactosyldiacylglyceride (RGDG) having a methylene-bridged palmitoleyl (17:Cyc) and a palmitoyl group (16:0) as the two acyl chains in a molecule [RGDG(17:Cyc,16:0)], together with the corresponding monosaccharide monogalactosyldiacylglyceride (MGDG). Here, we report changes in the structure and composition of the glycolipids that are dependent upon the temperature and period of cultivation. With a decrease in temperature to 25 °C, the two major glycolipids were almost completely eliminated, and MGDG with a palmitoleyl (16:1) and a (16:0) group concomitantly became the major glycolipid. MGDG(16:1,16:0) corresponded to the removal of an α-rhamnosyl and a cyclopropyl methylene group from RGDG(17:Cyc,16:0) and the lack of the CH(2) group in MGDG(17:Cyc,16:0). The structural conversion was almost reversible when the Cba. tepidum adapted to low and high temperatures was cultured again at 45 and 25 °C, respectively. Moreover, during this cultivation, the structure and composition of glycolipids were sequentially changed: MGDG(16:1,16:0), MGDG(17:Cyc,16:0), and RGDG(17:Cyc,16:0) predominated in the exponential, stationary and late phases of the cultivation, respectively. On the basis of these time-dependent changes, the unique disaccharide RGDG(17:Cyc,16:0) was thought to be created by the site-specific transfer of an α-rhamnosyl group to MGDG(17:Cyc,16:0) after insertion of a methylene group into the precursor MGDG(16:1,16:0). These culturing temperature- and time-dependent changes in glycolipids at the molecular level allow us to discuss their biosynthesis as well as physiological function in green photosynthetic bacteria.


Subject(s)
Chlorobium/metabolism , Glycolipids/chemistry , Chlorobium/growth & development , Chlorobium/physiology , Chromatography, High Pressure Liquid , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Photosynthesis , Spectrometry, Mass, Electrospray Ionization , Temperature , Time Factors
17.
Photosynth Res ; 107(2): 133-8, 2011 Feb.
Article in English | MEDLINE | ID: mdl-21161597

ABSTRACT

Bacteriochlorophyll(BChl)-f which has not yet been found in natural phototrophs was prepared by chemically modifying chlorophyll-b. The retention time of reverse-phase high-performance liquid chromatography of the synthetic monomeric BChl-f as well as its visible absorption and fluorescence emission spectra in a solution were identified and compared with other naturally occurring chlorophyll pigments obtained from the main light-harvesting antenna systems of green sulfur bacteria, BChls-c/d/e. Based on the above data, BChl-f was below the level of detection in three strains of green photosynthetic bacteria producing BChl-e.


Subject(s)
Bacteriochlorophylls/biosynthesis , Chlorobi/metabolism , Bacteriochlorophylls/chemical synthesis , Bacteriochlorophylls/chemistry , Chlorophyll/chemistry , Chlorophyll/isolation & purification , Chromatography, High Pressure Liquid , Spinacia oleracea/chemistry
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