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1.
Ann Work Expo Health ; 68(3): 280-294, 2024 Mar 15.
Article in English | MEDLINE | ID: mdl-38266254

ABSTRACT

INTRODUCTION: Epoxy-based resin formulations are a frequent cause of allergic and irritant contact dermatitis in the construction and painting industries. Cases of epoxy resin contact dermatitis continue to persist across many sectors and are likely attributable to the growing use of epoxy products, including epoxy-based anti-corrosion coatings and inadequate skin protection. There are no published performance data against epoxy resins for garment materials and gloves to guide proper material selection in the workplace. OBJECTIVES: The objective of this study was to evaluate the resistance of 5 protective garment materials against permeation and penetration by bisphenol A diglycidyl ether and its higher oligomers found commonly in epoxy-based anti-corrosion coatings. METHODS: Five disposable garment materials were evaluated for resistance to bisphenol A diglycidyl ether monomers and oligomers during contact with epoxy-based anti-corrosion coatings, including latex gloves, nitrile gloves, Tyvek coveralls, polypropylene/polyethylene (PP/PE) coveralls, and a cotton T-shirt. A permeation test cell system was used to evaluate each garment material against an epoxy-based zinc-rich primer and an epoxy-based intermediate coating using a realistic application method. Glass fiber filters were used to collect permeating and penetrating epoxy resin during a 120-min test period. Bisphenol A diglycidyl ether quantification was performed with high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. Paint loading, coating thickness, and homogeneity were assessed on polytetrafluoroethylene filters sprayed in series in permeation test cells. RESULTS: Latex gloves provided the least resistance to permeation by BADGE in coating formulations, with a maximum cumulative permeation over the 2-h test interval of 21.7 ng cm-2 with the primer and 513.8 ng cm-2 with the intermediate coating product. Nitrile gloves were not permeated by either coating formulation. The Tyvek coveralls provided greater protection as compared to the PP/PE coveralls. The cotton T-shirt was penetrated by bisphenol A diglycidyl ether more frequently than any of the tested garment materials and resulted in a maximum cumulative penetration of 128 ng cm-2 with the primer and 28.0 ng cm-2 with the intermediate coating. CONCLUSION: Although all the garment materials evaluated during this study provided sufficient protection to prevent cumulative permeation in excess of the established acceptable permeation thresholds, the use of nitrile gloves and Tyvek coverall is highly recommended to minimize skin exposure to bisphenol A diglycidyl ether. We recommend cotton T-shirts to be used under Tyvek coveralls as a secondary layer of skin protection and for added comfort, but not as a primary protection layer.


Subject(s)
Benzhydryl Compounds , Dermatitis, Contact , Epoxy Compounds , Occupational Exposure , Humans , Epoxy Resins , Latex , Occupational Exposure/analysis , Protective Clothing , Nitriles
2.
Ann Work Expo Health ; 67(9): 1088-1098, 2023 11 28.
Article in English | MEDLINE | ID: mdl-37665647

ABSTRACT

Agricultural workers frequently experience potentially hazardous exposure to non-ionizing radiation from both solar and artificial sources, and measurement of this exposure can be expensive and impractical for large populations. This project develops and evaluates a vegetative radiative transfer model (VRTM) to predict irradiance in a grow room of an indoor cannabis farm. The model uses morphological characteristics of the crop, manufacturer provided lamp emissions data, and dimensional measurements of the grow room and cannabis hedgerows to predict irradiance. A linear regression comparing model predictions with the measurements taken by a visible light spectroradiometer had slopes within 23% of unity and R2 values above 0.88 for visible (400-700 nm), blue (400-500 nm), green (500-600 nm), and red (600-700 nm) wavelength bands. The excellent agreement between the model and the measured irradiance in the cannabis farm grow room supports the potential of using VRTMs to predict irradiance and worker exposure in agricultural settings. Because there is no mechanistic difference between visible and other non-ionizing wavelengths of radiation in regards to mechanisms of radiative transfer, the model developed herein for visible wavelengths of radiation should be generalizable to other radiation bands including infrared and ultraviolet radiation.


Subject(s)
Cannabis , Occupational Exposure , Humans , Ultraviolet Rays , Farms , Sunlight
3.
Sensors (Basel) ; 22(22)2022 Nov 15.
Article in English | MEDLINE | ID: mdl-36433426

ABSTRACT

Available wearable dosimeters suffer from spectral mismatch during the measurement of broadband UV and visible radiation in environments that receive radiation from multiple sources emitting differing spectra. We observed this type of multi-spectra environment in all five Washington State cannabis farms visited during a field study investigating worker exposure to ultraviolet radiation in 2018. Spectroradiometers do not suffer from spectral mismatch in these environments, however, an extensive literature review conducted at the time of writing did not identify any spectroradiometers that were directly deployable as wearable dosimetry devices. To close this research gap, we developed a microcontroller system and platform that allows for researchers to mount and deploy the Ocean Insight Flame-S Spectroradiometer as a wearable device for measurement of UV and visible wavelengths (300 to 700 nm). The platform validation consisted of comparing measurements taken under platform control with measurements taken with the spectrometer controlled by a personal computer running the software provided by the spectroradiometer manufacturer. Three Mann-Whitney U-Tests (two-tailed, 95% CI), one for each intensity condition, compared the central tendency between the total spectral power (TSP), the integral of a spectrum measurement, measured under both control schemas. An additional analysis of per pixel agreement and overall platform stability was performed. The three Mann-Whitney tests returned no significant difference between the set of TSPs for each filter condition. These results suggest that the spectroradiometer takes measurements of equivalent accuracy under both control schemas, and can be deployed as a wearable device for the measurement of wavelength resolved UV and visible radiation.


Subject(s)
Ultraviolet Rays , Wearable Electronic Devices , Radiometry , Light , Radiation Dosimeters
4.
IEEE Open J Eng Med Biol ; 2: 187-197, 2021.
Article in English | MEDLINE | ID: mdl-34734189

ABSTRACT

GOAL: The impact of hyperthermia (HT) method on tumor drug uptake with thermosensitive liposomes (TSL) is not well understood. METHODS: We created realistic three-dimensional (3-D) computer models that simulate TSL-encapsulated doxorubicin (TSL-DOX) delivery in mouse tumors with three HT methods (thermistor probe (T), laser (L) and water bath (WB), at 15 min and 60 min HT duration), with corroborating in vivo studies. RESULTS: Average computer model-predicted tumor drug concentrations (µg/g) were 8.8(T, 15 min), 21.0(T, 60 min), 14.1(L, 15 min), 25.2(L, 60 min), 9.4(WB, 15 min), and 8.7(WB, 60 min). Tumor fluorescence was increased by 2.6 × (T) and 1.6 × (L) when HT duration was extended from 15 to 60 min (p < 0.05), with no increase for WB HT. Pharmacokinetic analysis confirmed that water bath HT causes rapid depletion of encapsulated TSL-DOX in systemic circulation due to the large heated tissue volume. CONCLUSIONS: Untargeted large volume HT causes poor tumor drug uptake from TSL.

5.
Environ Justice ; 14(4): 298-314, 2021 Aug 01.
Article in English | MEDLINE | ID: mdl-34484558

ABSTRACT

Background: Environmental racism, community stressors, and age-related susceptibility play a significant role in environmental inequality. The goal of this article was to use an inequality index (II) to assess the level of equality in environmental threats and hazards based on race, poverty, and age in Washington State. Methods: Using the Washington Environmental Health Disparities Map, we quantified the level of disproportionate burdens on communities with greater populations of people of color, people in poverty, children younger than 5, and people older than 65 using 3 cumulative environmental indices and 10 individual environmental indicators. Results: Census tracts with a higher proportion of people of color and those with people living below 185% federal poverty levels were found to be disproportionately burdened by environmental threats (II = -0.175 and II = -0.167, respectively, p < 0.001). Individual environmental indicators were found to disproportionately burden communities of color and low-income communities. Children younger than 5 were also disproportionately burdened by cumulative environmental indices (II = -0.076, p < 0.001) and individual indicators. Our analysis did not show disproportionate burden of environmental health threats based on the proportion of people older than 65 (II = 0.124, p < 0.001). Discussion: The disproportionate burden of the cumulative environmental threats on communities of color and low-income communities in this study corroborates similar analyses. These findings can be applied in policy and regulatory actions to correct the distributive environmental disparities. Conclusion: We found much higher burdens among historically marginalized communities and children who are more susceptible to environmental threats and hazards.

6.
Environ Health ; 20(1): 26, 2021 03 15.
Article in English | MEDLINE | ID: mdl-33722241

ABSTRACT

BACKGROUND: Pesticides play an important role in protecting the food supply and the public's health from pests and diseases. By their nature, pesticides can be toxic to unintended target organisms. Changing winds contribute to pesticide drift- the off-target movement of pesticides-and can result in occupational and bystander illness. METHODS: We systematically linked historical weather data to documented pesticide drift illnesses. We used Washington State Department of Health data to identify 252 drift events that included 690 confirmed cases of illness from 2000 to 2015. To characterize wind speed and direction at the time of the events, we paired these data with meteorological data from a network of 171 state weather stations. We report descriptive statistics and the spatio-temporal extent of drift events and compare applicator-reported weather conditions to those from nearby meteorological stations. RESULTS: Most drift events occurred in tree fruit (151/252 = 60%). Ground spraying and aerial applications accounted for 68% and 23% of events, respectively; 69% of confirmed cases were workers, and 31% were bystanders. Confirmed cases were highest in 2014 (129) from 22 events. Complete applicator spray records were available for 57 drift events (23%). Average applicator-reported wind speeds were about 0.9 m •sec- 1 (2 mi •hr- 1) lower than corresponding speeds from the nearest weather station values. CONCLUSIONS: Drift events result from a complex array of factors in the agricultural setting. We used known spatio-temporal aspects of drift and historical weather data to characterize these events, but additional research is needed to put our findings into practice. Particularly critical for this analysis is more accurate and complete information about location, time, wind speed, and wind direction. Our findings can be incorporated into new training materials to improve the practice of pesticide application and for better documentation of spray drift events. A precision agriculture approach offers technological solutions that simplify the task of tracking pesticide spraying and weather conditions. Public health investigators will benefit from improved meteorological data and accurate application records. Growers, applicators, and surrounding communities will also benefit from the explanatory and predictive potential of wind ramping studies.


Subject(s)
Air Pollutants/adverse effects , Environmental Exposure/adverse effects , Pesticides/adverse effects , Wind , Agriculture , Humans , Washington
7.
Environ Sci Technol ; 55(5): 2847-2858, 2021 03 02.
Article in English | MEDLINE | ID: mdl-33544581

ABSTRACT

The Mobile ObserVations of Ultrafine Particles study was a two-year project to analyze potential air quality impacts of ultrafine particles (UFPs) from aircraft traffic for communities near an international airport. The study assessed UFP concentrations within 10 miles of the airport in the directions of aircraft flight. Over the course of four seasons, this study conducted a mobile sampling scheme to collect time-resolved measures of UFP, CO2, and black carbon (BC) concentrations, as well as UFP size distributions. Primary findings were that UFPs were associated with both roadway traffic and aircraft sources, with the highest UFP counts found on the major roadway (I-5). Total concentrations of UFPs alone (10-1000 nm) did not distinguish roadway and aircraft features. However, key differences existed in the particle size distribution and the black carbon concentration for roadway and aircraft features. These differences can help distinguish between the spatial impact of roadway traffic and aircraft UFP emissions using a combination of mobile monitoring and standard statistical methods.


Subject(s)
Air Pollutants , Air Pollution , Air Pollutants/analysis , Air Pollution/analysis , Aircraft , Airports , Environmental Monitoring , Particle Size , Particulate Matter/analysis , Vehicle Emissions/analysis
8.
Biomacromolecules ; 22(2): 275-288, 2021 02 08.
Article in English | MEDLINE | ID: mdl-33332959

ABSTRACT

Vascularizing printed tissues is a critical challenge in bioprinting. While protein-based hydrogel bioinks have been successfully used to bioprint microvasculature, their compositions are ill-defined and subject to batch variation. Few studies have focused on engineering proangiogenic bioinks with defined properties to direct endogenous microvascular network formation after printing. Here, a peptide-functionalized alginate hydrogel bioink with defined mechanical, rheological, and biochemical properties is developed for direct bioprinting of microvascularized tissues. An integrin-binding peptide (RGD) and a vascular endothelial growth factor-mimetic peptide with a protease-sensitive linker are conjugated onto a biodegradable alginate to synergistically promote vascular morphogenesis and capillary-scale endothelial tube formation. Partial ionic crosslinking before printing converts the otherwise unprintable hydrogel into a viscoelastic bioink with excellent printability and cytocompatibility. We use the bioink to fabricate a compartmentalized vascularized tissue construct, wherein we observe pericyte-endothelial cell colocalization and angiogenic sprouting across a tissue interface, accompanied by deposition of fibronectin and collagen in vascular and tissue components, respectively. This study provides a tunable and translational "off-the-shelf" hydrogel bioink with defined composition for vascularized bioprinting.


Subject(s)
Bioprinting , Hydrogels , Microvessels , Printing, Three-Dimensional , Tissue Engineering , Tissue Scaffolds , Vascular Endothelial Growth Factor A
9.
Methods Mol Biol ; 2367: 235-247, 2021.
Article in English | MEDLINE | ID: mdl-32789778

ABSTRACT

With the realization that mechanical forces mediate many biological processes and contribute to disease progression, researchers are focusing on developing new methods to understand the role of mechanotransduction in biological systems. Despite recent advances in stretching devices that analyze the effects of mechanical strain in vitro, there are still possibilities to develop new equipment. For example, many of these devices tend be expensive, whereas few have been designed to assess the effects of mechanical strain driven by the extracellular matrix (ECM) to epithelial cell monolayers and to cell-cell adhesion. In this chapter, we introduce a cost-efficient, user-friendly, 3D-printed stretching device that can be used to test the effects of mechanical strain on cultured epithelial cells. Evaluation of the device using speckle-tracking shows homogeneous strain distribution along the horizontal plane of membranes at 2.5% and 5% strains, supporting the reliability of the device. Since cell-cell junctions are mechanosensitive protein complexes, we hereby used this device to examine effects on cell-cell adhesion. For this, we used colon epithelial Caco2 cell monolayers that well-differentiate in culture and form mature adherens junctions. Subjecting Caco2 cells to 2.5% and 5% strain using our device resulted in significant reduction in the localization of the core adherens junction component E-cadherin at areas of cell-cell contact and its increased translocation to the cytoplasm, which in agreement with other methodologies showing that increased ECM-driven strain negatively affects cell-cell adhesion. In summary, we here present a new, cost-effective, homemade device that can be reliably used to examine effects of mechanical strain on epithelial cell monolayers and cell-cell adhesion, in vitro.


Subject(s)
Stretchers , Adherens Junctions , Caco-2 Cells , Cadherins , Cell Adhesion , Epithelial Cells , Humans , Mechanotransduction, Cellular , Printing, Three-Dimensional , Reproducibility of Results
10.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 5021-5024, 2020 07.
Article in English | MEDLINE | ID: mdl-33019114

ABSTRACT

Thermosensitive liposomes (TSL) are nanoparticles that can encapsulate therapeutic drugs, and release those drugs when exposed to hyperthermic temperatures (>40 °C). Combined with localized hyperthermia, TSL enable focused drug delivery. In this study, we created a three-dimensional (3D) computer model for simulating delivery with TSL-encapsulated doxorubicin (TSL-Dox) to mouse tumors. A mouse hind limb was scanned by a 3D scanner and the resulting geometry was imported into finite element modeling software, with a virtual tumor added. Then, heating by a surface probe was simulated. Further, a drug delivery model was coupled to the heat transfer model to simulate drug delivery kinetics. For comparison, experimental studies in gel phantoms and in vivo fluorescence imaging studies in mice carrying lung tumor xenografts were performed. We report the tissue temperature profile, drug concentration profile and compare the experimental studies with the computer model. The thermistor produced very localized heating that resulted in highest drug delivery to regions near the probe. The average tumor temperature was 38.2˚C (range 34.4-43.4˚C), and produced an average tumor drug concentration of 11.8 µg/g (0.3-28.1 µg/g) after 15 min heating, and 25.6 µg/g (0.3-52 µg/g), after 60 min heating. The computer model reproduced the temperature profile compared to phantom experiments (mean error 0.71 °C, range 0.59-1.25 °C), as well as drug delivery profile as compared to in vivo studies. Our results suggest feasibility of using this approach to model drug delivery in preclinical studies with accurate model geometry.


Subject(s)
Hyperthermia, Induced , Liposomes , Animals , Antibiotics, Antineoplastic/therapeutic use , Computer Simulation , Drug Delivery Systems , Mice
11.
Chem Rev ; 120(19): 10887-10949, 2020 10 14.
Article in English | MEDLINE | ID: mdl-32867470

ABSTRACT

Microvasculature functions at the tissue and cell level, regulating local mass exchange of oxygen and nutrient-rich blood. While there has been considerable success in the biofabrication of large- and small-vessel replacements, functional microvasculature has been particularly challenging to engineer due to its size and complexity. Recently, three-dimensional bioprinting has expanded the possibilities of fabricating sophisticated microvascular systems by enabling precise spatiotemporal placement of cells and biomaterials based on computer-aided design. However, there are still significant challenges facing the development of printable biomaterials that promote robust formation and controlled 3D organization of microvascular networks. This review provides a thorough examination and critical evaluation of contemporary biomaterials and their specific roles in bioprinting microvasculature. We first provide an overview of bioprinting methods and techniques that enable the fabrication of microvessels. We then offer an in-depth critical analysis on the use of hydrogel bioinks for printing microvascularized constructs within the framework of current bioprinting modalities. We end with a review of recent applications of bioprinted microvasculature for disease modeling, drug testing, and tissue engineering, and conclude with an outlook on the challenges facing the evolution of biomaterials design for bioprinting microvasculature with physiological complexity.


Subject(s)
Biocompatible Materials/chemistry , Bioprinting , Microvessels , Printing, Three-Dimensional , Tissue Engineering , Humans
12.
Sci Adv ; 6(28): eaaz5894, 2020 07.
Article in English | MEDLINE | ID: mdl-32923589

ABSTRACT

Biologically active ligands (e.g., RGDS from fibronectin) play critical roles in the development of chemically defined biomaterials. However, recent decades have shown only limited progress in discovering novel extracellular matrix-protein-derived ligands for translational applications. Through motif analysis of evolutionarily conserved RGD-containing regions in laminin (LM) and peptide-functionalized hydrogel microarray screening, we identified a peptide (a1) that showed superior supports for endothelial cell (EC) functions. Mechanistic studies attributed the results to the capacity of a1 engaging both LM- and Fn-binding integrins. RNA sequencing of ECs in a1-functionalized hydrogels showed ~60% similarities with Matrigel in "vasculature development" gene ontology terms. Vasculogenesis assays revealed the capacity of a1-formulated hydrogels to improve EC network formation. Injectable alginates functionalized with a1 and MMPQK (a vascular endothelial growth factor-mimetic peptide with a matrix metalloproteinase-degradable linker) increased blood perfusion and functional recovery over decellularized extracellular matrix and (RGDS + MMPQK)-functionalized hydrogels in an ischemic hindlimb model, illustrating the power of this approach.


Subject(s)
Hydrogels , Vascular Endothelial Growth Factor A , Animals , Conserved Sequence , Extracellular Matrix , Ligands , Peptides/pharmacology
13.
Transl Oncol ; 13(6): 100775, 2020 Jun.
Article in English | MEDLINE | ID: mdl-32408199

ABSTRACT

Breast cancer patients presenting with symptomatic brain metastases have poor prognosis, and current chemotherapeutic agents are largely ineffective. In this study, we evaluated the hypomethylating agent azacitidine (AZA) for its potential as a novel therapeutic in preclinical models of brain metastasis of breast cancer. We used the parental triple-negative breast cancer MDA-MB-231 (231) cells and their brain colonizing counterpart (231Br) to ascertain phenotypic differences in response to AZA. We observed that 231Br cells have higher metastatic potential compared to 231 cells. With regard to therapeutic value, the AZA IC50 value in 231Br cells is significantly lower than that in parental cells (P < .01). AZA treatment increased apoptosis and inhibited the Wnt signaling transduction pathway, angiogenesis, and cell metastatic capacity to a significantly higher extent in the 231Br line. AZA treatment in mice with experimental brain metastases significantly reduced tumor burden (P = .0112) and increased survival (P = .0026) compared to vehicle. Lastly, we observed a decreased expression of keratin 18 (an epithelial maker) in 231Br cells due to hypermethylation, elucidating a potential mechanism of action of AZA in treating brain metastases from breast cancer.

14.
Article in English | MEDLINE | ID: mdl-32325838

ABSTRACT

Uranium is naturally found in the environment as a radioactive metal element with high concentrations in the Southwestern US. In this region is the Navajo Nation, which spans approximately 69,930 square kilometers. A decay product of uranium is radon gas, a lung carcinogen that has no color, odor, or taste. Radon gas may pass from soil into homes; and, indoor accumulation has been associated with geographical location, seasonality, home construction materials, and home ventilation. A home and indoor radon survey was conducted from November 2014 through May 2015, with volunteers who reported residence on the Navajo Nation. Home geolocation, structural characteristics, temperature (°C) during radon testing, and elevation (meters) were recorded. Short-term indoor radon kits were used to measure indoor radon levels. 51 homes were measured for indoor radon levels, with an arithmetic mean concentration of 60.5 Becquerels per cubic meter (Bq/m3) (SD = 42.7). The mean indoor radon concentrations (Bq/m3) by house type were: mobile, 29.0 (SD = 22.9); wood, 58.6 (SD = 36.0); hogan, 74.0 (SD = 0.0); homes constructed of cement and wood, 82.6 (SD = 3.5); and homes constructed of concrete and cement, 105.7 (SD = 55.8). A key observation is that house construction type appears to be associated with the mean home indoor radon concentration. This observation has been published in that the basic structural make-up of the home may affect home ventilation and therefore indoor radon concentration levels.


Subject(s)
Air Pollutants, Radioactive , Air Pollution, Indoor , Construction Materials , Housing , Radon , Air Pollutants, Radioactive/analysis , Air Pollution, Indoor/analysis , Environment , Humans , Indians, North American , Radon/analysis , Seasons , Temperature
15.
Environ Sci Technol ; 54(7): 4286-4294, 2020 04 07.
Article in English | MEDLINE | ID: mdl-32150678

ABSTRACT

This study examines the feasibility of the in situ calibration of instruments for fleet vehicle-based mobile monitoring of ultrafine particles (UFPs) and black carbon (BC) by comparing rendezvous vehicle measurements. Two vehicles with identical makes and models of UFP and BC monitors as well as GPS receivers were sampled within 140 m of each other for 2 h in total during winter in Seattle, Washington. To identify an optimal intervehicle distance for rendezvous calibration, 6 different buffers within 0-140 m for UFP monitors and 5 different buffers within 0-90 m for BC monitors were chosen, and the results of calibration were compared against a reference scenario, which consisted of mobile colocation measurements with both sets of the UFP and BC monitors deployed in one of the vehicles. Results indicate that the optimal distances for rendezvous calibration are 10-80 m for UFP monitors and 0-30 m for BC monitors. In comparison with the mobile colocation calibration, the rendezvous calibration shows a normalized root mean squared deviation of 6-14% and a normalized mean absolute deviation of 4-8% for these monitors. Criteria for applying a rendezvous calibration approach are presented, and an extension of this approach to an instrumented fleet of mobile monitoring vehicles is discussed.


Subject(s)
Air Pollutants , Air Pollution , Calibration , Environmental Monitoring , Particulate Matter , Vehicle Emissions , Washington
16.
Ann Work Expo Health ; 64(1): 25-37, 2020 01 01.
Article in English | MEDLINE | ID: mdl-31786605

ABSTRACT

Pesticide spray drift represents an important exposure pathway that may cause illness among orchard workers. To strike a balance between improving spray coverage and reducing drift, new sprayer technologies are being marketed for use in modern tree canopies to replace conventional axial fan airblast (AFA) sprayers that have been used widely since the 1950s. We designed a series of spray trials that used mixed-effects modeling to compare tracer-based drift volume levels for old and new sprayer technologies in an orchard work environment. Building on a smaller study of 6 trials (168 tree rows) that collected polyester line drift samples (n = 270 measurements) suspended on 15 vertical masts downwind of an AFA sprayer application, this study included 9 additional comparison trials (252 tree rows; n = 405 measurements) for 2 airblast tower sprayers: the directed air tower (DAT) and the multi-headed fan tower (MFT). Field-based measurements at mid (26 m) and far (52 m) distances showed that the DAT and MFT sprayers had 4-15 and 35-37% less drift than the AFA. After controlling for downwind distance, sampling height, and wind speed, model results indicated that the MFT [-35%; 95% confidence interval (CI): -22 and -49%; P < 0.001] significantly reduced drift levels compared to the AFA, but the DAT did not (-7%; 95% CI: -19 and 6%; P = 0.29). Tower sprayers appear to be a promising means by which to decrease drift levels through shorter nozzle-to-tree canopy distances and more horizontally directed aerosols that escape the tree canopy to a lesser extent. Substitution of these new technologies for AFA sprayers is likely to reduce the frequency and magnitude of pesticide drift exposures and associated illnesses. These findings, especially for the MFT, may fit United States Environmental Protection Agency's Drift Reduction Technology (DRT) one-star rating of 25-50% reduction. An 'AFA buyback' incentive program could be developed to stimulate wider adoption of new drift-reducing spray technologies. However, improved sprayer technologies alone do not eliminate drift. Applicator training, including proper sprayer calibration and maintenance, and application exclusion zones (AEZs) can also contribute to minimizing the risks of drift exposure. With regard to testing DRTs and establishing AEZs, our study findings demonstrate the need to define the impact of airblast sprayer type, orchard architecture, sampling height, and wind speed.


Subject(s)
Agriculture/instrumentation , Occupational Exposure , Pesticides , Aerosols , Models, Theoretical , Pesticides/analysis , Technology , Wind
17.
Regul Toxicol Pharmacol ; 110: 104504, 2020 Feb.
Article in English | MEDLINE | ID: mdl-31655092

ABSTRACT

The benchmark dose has been frequently recommended for the creation of points of departure for regulatory dose limits, but many regulations, including pesticide risk assessment and registration in the United States, continues to rely on NOAEL methods as the OECD toxicological standard methods recommend. This study used data from studies in support of pesticide registration for eight different compounds to build dose-response models and calculate benchmark doses and confidence limits. The results were compared to the NOAEL of the same study. A probabilistic estimate of dose was compared with all points of departure to demonstrate differences in the protective ability of each different selected limit. While neither the BMD/BMDL nor the NOAEL was consistently more protective, the advantage of using the BMD in quantifying the uncertainty of the point of departure is highlighted, and the feasibility of using current OECD-guideline studies for derivation of a BMD is demonstrated in these cases.


Subject(s)
Models, Theoretical , Occupational Exposure/adverse effects , Pesticides/toxicity , Risk Assessment/methods , Agriculture , Animals , Benchmarking , Farmers , Fruit , Humans , No-Observed-Adverse-Effect Level
18.
Article in English | MEDLINE | ID: mdl-31766307

ABSTRACT

Communities across Washington State have expressed the need for neighborhood-level information on the cumulative impact of environmental hazards and social conditions to illuminate disparities and address environmental justice issues. Many existing mapping tools have not explicitly integrated community voice and lived experience as an integral part of their development. The goals of this project were to create a new community-academic-government partnership to collect and summarize community concerns and to develop a publicly available mapping tool that ranks relative environmental health disparities for populations across Washington State. Using a community-driven framework, we developed the Washington Environmental Health Disparities Map, a cumulative environmental health impacts assessment tool. Nineteen regularly updated environmental and population indicators were integrated into the geospatial tool that allows for comparisons of the cumulative impacts between census tracts. This interactive map provides critical information for the public, agencies, policymakers, and community-based organizations to make informed decisions. The unique community-academic-government partnership and the community-driven framework can be used as a template for other environmental and social justice mapping endeavors.


Subject(s)
Community Participation , Decision Making , Environmental Health , Health Status Disparities , Environmental Exposure , Humans , Residence Characteristics , Social Justice , Socioeconomic Factors , Washington
19.
Ann Work Expo Health ; 63(5): 592-603, 2019 05 21.
Article in English | MEDLINE | ID: mdl-31066890

ABSTRACT

BACKGROUND: Polyurethanes are a class of isocyanate-based organic coatings commonly used to control corrosion on high-value metallic structures. Despite their widespread use, dermal exposure to these isocyanate-containing coatings presents a significant occupational health risk to workers, including the development of allergic and irritant contact dermatitis and systemic sensitization. At present, little is known about the effectiveness of the protective garments commonly used to prevent dermal exposure to polyurethane coatings in construction trades. OBJECTIVES: The primary objective of this study was to measure the permeation and penetration of isocyanates from polyurethane anticorrosion coatings though a selection of protective garments. In addition, a standardized spray procedure using a fixed-position spraying technique was evaluated as an option to minimize variability in coating application. METHODS: Five disposable garment materials were evaluated for resistance to isocyanates during this study: latex gloves (0.076 mm), nitrile gloves (0.078 mm), Tyvek coveralls (0.105 mm), polypropylene/polyethylene (PP/PE) coveralls (0.116 mm), and a cotton t-shirt (0.382 mm). A permeation test cell system was used to evaluate each garment material against two products: a polyurethane zinc-rich primer based on 4,4'-methylene diphenyl diisocyanate and an aliphatic finish coating based on prepolymers of 1,6-hexamethylene diisocyanate. Glass fiber filters pretreated with 1-(9-anthracenylmethyl)piperazine were used to collect penetrating isocyanates during the 120-min test period, which were analyzed by liquid chromatography-tandem mass spectrometry. Polytetrafluoroethylene loading filters were sprayed in series with permeation test cells and analyzed gravimetrically to assess the homogeneity of coating application. RESULTS: The latex gloves demonstrated the highest rate of isocyanate permeation of all evaluated garments during testing with both coatings (primer: 27.38 ng cm-2 min-1; finish coating: 7.39 ng cm-2 min-1). Nitrile gloves were much more resistant than latex gloves (primer: 1.89 ng cm-2 min-1; finish coating: 1.26 ng cm-2 min-1) and were not permeated by the finish coating until after 15 min. The PP/PE coverall provided the most consistent resistance to both coatings (primer: 0.08 ng cm-2 min-1; finish coating: 1.27 ng cm-2 min-1), whereas the Tyvek coverall was readily permeated by the primer (primer: 3.47 ng cm-2 min-1; finish coating: 0.87 ng cm-2 min-1). The cotton t-shirt was rapidly permeated by the primer during the first 5 min of exposure (primer: 146.65 ng cm-2 min-1; finish coating: 4.64 ng cm-2 min-1). In addition, the fixed-position spraying technique used during this study demonstrated a significant reduction in loading variability within each batch of test cells when compared to manual spray application. CONCLUSION: Nitrile gloves demonstrated superior resistance to both isocyanate-containing coatings in comparison to latex gloves. Although both coverall materials were resistant to permeating isocyanate within the established thresholds, the PP/PE coverall provided more consistent resistance to both coatings. Owing to the cotton t-shirt's high rate of penetration with both coatings, it is recommended only as a secondary barrier. Study results showed that the use of fixed-position spray techniques provided consistent and reproducible results within each batch of test cells. Additional test design modifications are necessary to further reduce variability between batches and ensure more consistent coating thickness.


Subject(s)
Isocyanates/analysis , Materials Testing/methods , Occupational Exposure/analysis , Polyurethanes/adverse effects , Protective Clothing/standards , Gloves, Protective/standards , Humans , Nitriles , Permeability , Textiles
20.
Chemosphere ; 222: 46-55, 2019 May.
Article in English | MEDLINE | ID: mdl-30690400

ABSTRACT

In Washington State, half of all pesticide-related illnesses in agriculture result from drift, the off-target movement of pesticides. Of these, a significant proportion involve workers on another farm and orchard airblast applications. We compared the spray drift exposure reduction potential of two modern tower sprayers - directed air tower (DAT) and multi-headed fan tower (MFT), in relation to a traditional axial fan airblast (AFA) sprayer. We employed real-time particle monitors (Dylos DC1100) during a randomized control trial of orchard spray applications. Sections of a field were randomly sprayed by three alternating spray technologies - AFA, DAT and MFT - while monitors sampled particulate matter above and below the canopy at various downwind locations in a neighboring field. Geometric mean particle mass concentrations (PMC) outside the intended spray area were elevated during all applications at all of our sampling distances (16-74 m, 51-244 ft). After adjusting for wind speed and sampling height, the 75th percentile (95% confidence interval) PMC level was significantly greater during spray events than background levels by 105 (93, 120) µg/m3, 49 (45, 54) µg/m3 and 26 (22, 31) µg/m3 during AFA, DAT and MFT applications, respectively. Adjusted PMC levels were significantly different between all three sprayers. In this study, tower sprayers significantly reduced spray drift exposures in a neighboring orchard field when compared to the AFA sprayer, with the MFT sprayer producing the least drift; however these tower sprayers did do not fully eliminate drift.


Subject(s)
Agriculture/methods , Environmental Monitoring/methods , Particulate Matter/analysis , Pesticides/analysis , Air Movements , Air Pollutants/analysis , Pesticides/toxicity , Technology/instrumentation , Washington , Wind
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