Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 12 de 12
Filter
Add more filters










Publication year range
1.
J Colloid Interface Sci ; 616: 81-92, 2022 Jun 15.
Article in English | MEDLINE | ID: mdl-35189506

ABSTRACT

Although photodynamic therapy (PDT) has been extensively studied as an established modality of cancer treatment, it still suffers from a few clinical limitations, such as skin phototoxicity and tumor hypoxia. To circumvent these hurdles, hollow silica mesoporous nanoparticles (HMSNs) loaded with photosensitizers were employed as the nanoplatform to construct multifunctional nanoparticles (NPs). Specifically, an ultra-uniform polydopamine (PDA) shell was highly controlled grown around HMSNs by photogenerated outwards-diffused 1O2, followed by conjugation of folic acid-poly(ethylene glycol) and chelation of Fe2+ ions. Thanks to the optimal thickness of light-absorbing PDA shell, the multifunctional NPs exhibited not only negligible skin phototoxicity but also efficient 1O2 generation and photothermal (PT)-enhanced •OH generation upon respective photoirradiation. Anti-tumor therapy was then performed on both 4 T1 tumor cells and tumor-bearing mice by the combination of 638 nm PDT and 808 nm PT-enhanced chemodynamic therapy (CDT). As a result, high therapeutic efficacy was achieved compared to single-modality therapy, with a cell inhibitory rate of 86% and tumor growth inhibition of 70.4% respectively. More interestingly, tumor metastasis was effectively inhibited by the synergetic treatment. These results convincingly demonstrate that our multifunctional NPs are very promising skin-safe PDT agents combined with CDT for efficient tumor therapy.


Subject(s)
Nanoparticles , Neoplasms , Photochemotherapy , Animals , Cell Line, Tumor , Indoles/pharmacology , Indoles/therapeutic use , Mice , Neoplasms/drug therapy , Photosensitizing Agents/pharmacology , Photosensitizing Agents/therapeutic use , Polymers/therapeutic use , Silicon Dioxide/therapeutic use
2.
Anal Bioanal Chem ; 412(11): 2579-2587, 2020 Apr.
Article in English | MEDLINE | ID: mdl-32076790

ABSTRACT

A series of Ru(II)-containing metallopolymers with different polypyridyl complexes, namely [Ru(N^N)2(L)](PF6)2 (L = bipyridine-branched polymer; N^N = bpy: 2,2'-bipyridine (Ru 1); phen: 1,10-phenanthroline (Ru 2); dpp: 4,7-diphenyl-1,10-phenanthroline (Ru 3)), were synthesized with the motive that adjusting π-conjugation length of ligands might produce competent luminescent oxygen probes. The three hydrophobic metallopolymers were studied with 1H NMR, UV-Vis absorption, and emission spectroscopy, and then were utilized to prepare biocompatible nanoparticles (NPs) via a nanoprecipitation method. Luminescent properties of the NPs were investigated against dissolved oxygen by steady-state and time-resolved spectroscopy respectively. Luminescence quenching of the three NPs all followed a linear behavior in the range of 0-43 ppm (oxygen concentration), but Ru 3-NPs exhibited the highest oxygen sensitivity (82%) and longest emission wavelength (λex = 460 nm; λem = 617 nm). In addition, external interferons from cellular environments (e.g., pH, temperature, and proteins) had been studied on Ru 3-NPs. Finally, dissolved oxygen in monolayer cells under normoxic/hypoxic conditions was clearly differentiated by using Ru 3-NPs as the luminescent sensor, and, more importantly, hypoxia within multicellular tumor spheroids was vividly imaged. These results suggest that such Ru(II)-containing metallopolymers are strong candidates for luminescent nanosensors towards hypoxia. Graphical abstract.


Subject(s)
Luminescent Agents/chemistry , Oxygen/analysis , Ruthenium/chemistry , Tumor Hypoxia , 2,2'-Dipyridyl/chemistry , HeLa Cells , Humans , Ligands , Luminescence , Luminescent Measurements/methods , Phenanthrolines/chemistry
3.
Nanotechnology ; 30(34): 345207, 2019 Aug 23.
Article in English | MEDLINE | ID: mdl-31035278

ABSTRACT

Tumor hypoxia severely reduces the efficiency of photodynamic therapy (PDT) through the insufficient supply of oxygen. In this work, we reported on a design of fluorinated nanophotosensitizers (NPSs) prepared by a facile reprecipitation-encapsulation method, with the aim of addressing the issue of hypoxia. The fluorinated NPSs consisted of a hybrid particle core of perfluorosiloxane-polystyrene, doped with a fluorinated photosensitizer, and a biocompatible poly-l-lysine shell. Compared with non-fluorinated counterpart NPSs that are similarly prepared except for the replacement of perfluorosiloxane with alkoxysilane, the fluorinated NPSs saturated with O2 exhibit approximately 3.5 fold higher singlet oxygen production yield and higher in vitro PDT efficiency due to the O2-carrying capability of intra-particle 'F-C' bonds.


Subject(s)
Nanoparticles/chemistry , Photosensitizing Agents/chemistry , Cell Hypoxia , Cell Survival/drug effects , Fluoridation , HeLa Cells , Humans , Lasers , Neoplasms/drug therapy , Oxygen/chemistry , Photochemotherapy , Photosensitizing Agents/pharmacology , Polylysine/chemistry , Polystyrenes/chemistry , Singlet Oxygen/chemistry , Singlet Oxygen/metabolism
4.
ACS Sens ; 4(4): 984-991, 2019 04 26.
Article in English | MEDLINE | ID: mdl-30859818

ABSTRACT

Metal complex-based luminescent oxygen nanosensors have been intensively studied for biomedical applications. In terms of monitoring dynamics of intracellular oxygen, however, high-quality nanosensors are still badly needed, because of stringent requirements on stability, biocompatibility and luminescence intensity, aside from oxygen sensitivity. In this paper, we reported a type of highly luminescent and stable oxygen nanosensors prepared from metallopolymer. First, a novel ruthenium(II)-containing metallopolymer was synthesized by chelating the oxygen probe [Ru(bpy)3]2+ with a bipyridine-branched hydrophobic copolymer, which was then doped into polymeric nanoparticles (NPs) by a reprecipitation method, followed by further conjugation to selectively target mitochondria (Mito-NPs). The resultant Mtio-NPs possessed a small hydrodynamic size of ∼85 nm, good biocompatibility and high stability resulting from PEGylation and stable nature of Ru-complex. Because the complexed [Ru(bpy)3]2+ homogeneously resided on particle surface, Mito-NPs exhibited strong luminescence at 608 nm that was free of aggregation-caused-quenching, the utmost oxygen sensitivity of free [Ru(bpy)3]2+ probe ( Q = 75%), and linear Stern-Volmer oxygen luminescence quenching plots. Taking advantage of the mitochondria-specific nanosensors, intracellular oxygenation and deoxygenation processes were real-time monitored for 10 min by confocal luminescence imaging, visualized by the gradual weakening (by more than 90%) and enhancing (by 50%) of the red emission, respectively.


Subject(s)
Acrylic Resins/chemistry , Luminescent Agents/chemistry , Mitochondria/metabolism , Nanoparticles/chemistry , Oxygen/analysis , Polystyrenes/chemistry , Acrylic Resins/chemical synthesis , Acrylic Resins/toxicity , Coordination Complexes/chemical synthesis , Coordination Complexes/chemistry , Coordination Complexes/toxicity , Hep G2 Cells , Humans , Luminescence , Luminescent Agents/chemical synthesis , Luminescent Agents/toxicity , Luminescent Measurements/methods , Microscopy, Confocal/methods , Nanoparticles/toxicity , Polystyrenes/chemical synthesis , Polystyrenes/toxicity , Proof of Concept Study , Ruthenium/chemistry , Ruthenium/toxicity
5.
Mikrochim Acta ; 185(5): 269, 2018 04 26.
Article in English | MEDLINE | ID: mdl-29700623

ABSTRACT

Sensing of intracellular singlet oxygen (1O2) is required in order to optimize photodynamic therapy (PDT). An optical nanoprobe is reported here for the optical determination of intracellular 1O2. The probe consists of a porous particle core doped with the commercial 1O2 probe 1,3-diphenylisobenzofuran (DPBF) and a layer of poly-L-lysine. The nanoparticle probes have a particle size of ~80 nm in diameter, exhibit good biocompatibility, improved photostability and high sensitivity for 1O2 in both absorbance (peak at 420 nm) and fluorescence (with excitation/emission peaks at 405/458 nm). Nanoprobes doped with 20% of DPBF are best suited even though they suffer from concentration quenching of fluorescence. In comparison with the commercial fluorescent 1O2 probe SOSG, 20%-doped DPBF-NPs (aged) shows higher sensitivity for 1O2 generated at an early stage. The best nanoprobes were used to real-time monitor the PDT-triggered generation of 1O2 inside live cells, and the generation rate is found to depend on the supply of intracellular oxygen. Graphical abstract A fluorescent nanoprobe featured with refined selectivity and improved sensitivity towards 1O2 was prepared from the absorption-based probe DBPF and used to real-time monitoring of the generation of intracellular 1O2 produced during PDT.


Subject(s)
Benzofurans/chemistry , Fluorescent Dyes/chemistry , Singlet Oxygen/metabolism , Benzofurans/radiation effects , Benzofurans/toxicity , Fluorescence , Fluorescent Dyes/radiation effects , Fluorescent Dyes/toxicity , Hep G2 Cells , Humans , Light , Nanoparticles/chemistry , Nanoparticles/radiation effects , Nanoparticles/toxicity , Photochemotherapy , Polylysine/chemistry , Polylysine/toxicity , Singlet Oxygen/analysis , Singlet Oxygen/chemistry , Spectrometry, Fluorescence/methods
6.
Methods Appl Fluoresc ; 4(3): 035001, 2016 07 28.
Article in English | MEDLINE | ID: mdl-28355161

ABSTRACT

In this work luminescent nanosensors specifically created for intracellular oxygen (ic-O2) were utilized to assess photodynamic therapy (PDT) -induced cell damages. Firstly, ic-O2 was demonstrated to be consumed much faster than extracellular O2 with respective O2 nanosensors. Using the ic-O2 nanosensors, PDT-treated cells with different degree of impairment were then resolved according to the oxygen consumption rate (OCR). The evolving trend of cytotoxicity derived from OCRs was in agreement with cell viability obtained from 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Moreover, the direct damage of PDT on cell mitochondria was successfully detected by monitoring respiration instantly after PDT treatment, which is actually beyond the scope of MTT assay. These results suggest that fluorescence sensing of ic-O2-associated cell respiration is promising and even may become a standardized method, complementary to MTT assay, to evaluate PDT-induced cytotoxicity.


Subject(s)
Oxygen/analysis , Apoptosis , Cell Line, Tumor , Cell Respiration , Cell Survival , Humans , Oxygen Consumption , Photochemotherapy , Photosensitizing Agents , Singlet Oxygen
7.
J Mater Chem B ; 4(25): 4482-4489, 2016 Jul 07.
Article in English | MEDLINE | ID: mdl-32263431

ABSTRACT

Zinc(ii) phthalocyanine (ZnPc) is a promising photosensitizer for PDT but suffers from aggregation in a physiological aqueous environment. In this paper, a class of biocompatible polymeric nanoparticles (NPs) was prepared to encapsulate ZnPc molecules. Mostly because of the planar structure, ZnPc molecules were difficult to be encapsulated into the polymeric NPs unless further coated with a thick poly-l-lysine (PLL) layer. The PLL shell endowed the NPs with good biocompatibility, efficient cellular uptake, and potential bioconjugation. The degree of aggregation (DOA) of ZnPc molecules in PLL-NPs was thoroughly investigated based on self-defined relative DOA, and a loading capacity of 4 wt% was deduced as the turning point for aggravating aggregation. Similarly, the optimal loading capacity of ZnPc was determined to be 4% according to the 1O2 generation rate, demonstrating the feasibility of the DOA approach. Polymers with large rigid units (PVK and PFO) were also utilized to relieve the aggregation of ZnPc in NPs. Taking advantage of the optimized ZnPc-loaded NPs, high PDT efficacy was demonstrated in HepG2 cells and in tumor-bearing mice as well. Both high in vitro and in vivo PDT efficacy and biocompatibility are demonstrated. Aside from affording a class of efficient biocompatible nanophotosensitizers, this work is also instructive to design other types of ZnPc-based nanocarriers, in which aggregation should be well considered.

8.
Biomed Res Int ; 2015: 245031, 2015.
Article in English | MEDLINE | ID: mdl-26539471

ABSTRACT

For most fluorescent oxygen sensors developed today, their fabrication process is either time-consuming or needs specialized knowledge. In this work, a robust fluorescent oxygen sensor is facilely constructed by dissolving pyrene molecules into CTAB aqueous solution. The as-prepared pyrene@micelle sensors have submicron-sized diameter, and the concentration of utilized pyrene can be reduced as low as 0.8 mM but still can exhibit dominant excimer emission. The excimer fluorescence is sensitive to dissolved oxygen in both intensity and lifetime, and the respective Stern-Volmer plot follows a nonlinear behavior justified by a two-site model. Because of the merits of large Stokes shift (~140 nm), easy fabrication, and robustness, the pyrene@micelle sensors are very attractive for practical determination of oxygen.


Subject(s)
Biosensing Techniques/methods , Oxygen/isolation & purification , Spectrometry, Fluorescence/methods , Fluorescence , Humans , Micelles , Pyrenes/chemistry
9.
Angew Chem Int Ed Engl ; 53(46): 12471-5, 2014 Nov 10.
Article in English | MEDLINE | ID: mdl-25044871

ABSTRACT

Cellular respiration is a worthwhile criterion to evaluate mitochondrial dysfunction by measuring the dissolved oxygen. However, most of the existing sensing strategies merely report extracellular (ec-) or intracellular (ic-) O2 rather than intramitochondrial (im-) O2 . Herein we present a method to assess tumor mitochondrial dysfunction with three phosphorescent nanosensors, which respond to ec-, ic-, and im-O2 . Time-resolved luminescence is applied to determine the respective oxygen consumption rates (OCRs) under varying respiratory conditions. Data obtained for the OCRs and on (intra)cellular O2 gradients demonstrate that mitochondria in tumor cells are distinctly less active than those of healthy cells, resulting from restrained glucose utilization of and physical injury to the mitochondria. We believe that such a site-resolved sensing strategy can be applied to numerous other situations, for example to evaluate the adverse effects of drug candidates.


Subject(s)
Luminescent Agents/analysis , Mitochondria/pathology , Nanoparticles/analysis , Neoplasms/metabolism , Oxygen/analysis , Cell Respiration , Hep G2 Cells , Humans , Luminescent Agents/metabolism , Mitochondria/metabolism , Nanoparticles/metabolism , Neoplasms/pathology , Oxygen/metabolism , Oxygen Consumption
10.
J Mater Chem B ; 1(38): 5143-5152, 2013 Oct 14.
Article in English | MEDLINE | ID: mdl-32261106

ABSTRACT

In this paper, we report a facile route to synthesize mitochondria-targeted core-shell nanoparticles (NPs). Firstly, PLL-coated NPs are prepared by a one-step reprecipitation-encapsulation method assisted by positively charged poly-l-lysine (PLL). The effect of the molecular weight of PLL on the formation of particles is studied in terms of morphology, size and zeta potential, and medium-sized PLL (MH-PLL) is proved to be the optimum one. By means of crosslinking with different amounts of glutaraldehyde, amino groups in MH-PLL-NPs are characterized by zeta potential and fluorescamine assay, respectively. The results indicate that in the PLL shell, only a small portion of amino groups (surface amino groups, SAGs) are available for conjugation, while the other groups exclusively contribute to zeta potential. Subsequently, a known mitochondriotropic ligand, triphenylphosphonium (TPP), is conjugated with SAG via a carbodiimide reaction, which is evaluated by NMR and absorption spectra, respectively. The TPP-MH-PLL-NPs exhibit a low cytotoxic effect tested by the MTT method, as well as efficient cellular uptake microscopically observed after a fluorescent dye, coumarin 6, is incorporated. Most importantly, the TPP-conjugated NPs can selectively target mitochondria, demonstrated by the merged z-stacked images in co-localization experiments with MitoTracker-stained mitochondria. Given that many hydrophobic species could be loaded into the particle core, TPP-MH-PLL-NPs are very promising as mitochondria-targeted nanocarriers for imaging or anti-cancer therapies.

11.
Guang Pu Xue Yu Guang Pu Fen Xi ; 28(5): 1012-5, 2008 May.
Article in Chinese | MEDLINE | ID: mdl-18720790

ABSTRACT

The emission spectra of SrAl12O19 : Pr3+ at temperature from 308 to 483 K were investigated under vacuum ultraviolet excitation. Both the dipole-forbidden 4f2 --> 4f2 transition and the dipole-allowed 4f5d --> 4f2 transitions were observed. The intensity of the 4f5d --> 4f2 emission relative to that of the 4f2 --> 4f2 emission increased with increasing temperature. A thermal equilibrium model was applied to the system consisting of the 1S0 state and the lowest 4f5d states of Pr3+, and the temperature dependence of emission spectra of Pr3+ in the Pr(3+)-doped SrAl12O19 was well explained.

12.
Guang Pu Xue Yu Guang Pu Fen Xi ; 26(2): 231-4, 2006 Feb.
Article in Chinese | MEDLINE | ID: mdl-16826894

ABSTRACT

La2O3: Eu(3+) nanoparticles were prepared by Gly assistant combustion synthesis with the sizes from 12-28 nm, and a characterization of XRD was done. Spectral properties of the nanoparticles were compared with the bulk. High resolution spectra were measured. Site selective excitation was employed to probe the local environments of Eu(3+) ions in La2O3 nanoparticles. The luminescent centers on the surface and the center of the nanoparticles were excited respectively. The spectra were related to surface information. The luminescence from C3v site and the site with lower symmetry on the surface was distinguished.

SELECTION OF CITATIONS
SEARCH DETAIL
...