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Acta Crystallogr Sect F Struct Biol Cryst Commun
; 66(Pt 12): 1674-6, 2010 Dec 01.
Article
in English
| MEDLINE
| ID: mdl-21139222
ABSTRACT
DEAD-box helicases are enzymes with an ATP-dependent RNA-unwinding function that are involved in a variety of cellular processes including RNA splicing, ribosome biogenesis and RNA degradation. In this study, the N-terminal domain of DEAD-box RNA helicase from Staphylococcus aureus strain Mu50 was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.60â Å resolution using a synchrotron-radiation source. The crystal belonged to space group P1, with unit-cell parameters a=70.81, b=80.23, c=86.25â Å, α=69.54, ß=66.54, γ=87.32°. The unit cell contained six molecules, with a corresponding VM of 2.91â Å3â Da(-1) and a solvent content of 56.1%.