ABSTRACT
The NUDT family is thought to play an important role in cancer growth and progression. However, the clinicopathologic significance and potential role of nucleotide diphosphate-linked X-component motif 21, NM_007006 (NUDT21) in pancreatic ductal adenocarcinoma (PDAC) remains largely unknown. In this study, we observed that NUDT21 was frequently up-expressed in PDAC. Clinical data revealed that its level positively correlated with poor survival of patients with PDAC. We found that knockdown of NUDT21 significantly inhibited cell proliferation and promoted apoptosis both in vitro and in vivo. Screening by microarray analysis and verifying by Western blot, we found that the EIF2 signaling pathway represented the main molecular mechanism underlying the effects of NUDT21 knockdown in PANC-1 cells, and PKR, HSPA5, EIF4E and DDIT3 may be its target genes. Thus, our results revealed for the first time that NUDT21, a valuable marker of PDAC prognosis, promotes tumor proliferation, inhibits cells apoptosis and might represent a potential target for gene-based therapy.
Subject(s)
Apoptosis/genetics , Carcinoma, Pancreatic Ductal/genetics , Cell Proliferation/genetics , Cleavage And Polyadenylation Specificity Factor/genetics , Eukaryotic Initiation Factor-2/metabolism , Biomarkers, Tumor/genetics , Carcinoma, Pancreatic Ductal/metabolism , Cell Line, Tumor , Cell Movement/genetics , Cleavage And Polyadenylation Specificity Factor/metabolism , Endoplasmic Reticulum Chaperone BiP , Gene Expression Regulation, Neoplastic , Humans , Male , Pancreatic Neoplasms , Pancreatic NeoplasmsABSTRACT
Rapamycin (RAPA), an inhibitor of mammalian target of rapamycin (mTOR), exhibits a high neuroprotective action against neurodegenerative diseases in mouse models. Since neuroinflammation has been shown to be involved in Alzheimer's disease (AD) development and progression, the aim of this study was to examine the anti-inflammatory role of RAPA in AD in vivo and in vitro, and investigate the underlying mechanisms. We found that amyloid-ß (Aß) induced neuronal inflammation and a remarkable increase in mTOR activity in in-vivo and in-vitro models of inflammation, suggesting the critical role of mTOR signaling in neuronal inflammation. In addition, administration of RAPA was found to down-regulate mTOR, p-mTOR, Nuclear factor kappa B (NF-κB) p65, p-p65, TNF-α, IL-1ß and Bax protein expression in Aß25-35- or lipopolysaccharides (LPS)-treated mice and cultured Neuro-2a (N2a) cells. Moreover, RAPA disrupted Aß25-35-induced nuclear translocation of mTOR and NF-κB. Our findings indicate that RAPA inhibits Aß25-35- or LPS-induced neuronal inflammation through suppressing mTOR signaling and reducing nuclear import of NF-κB.
Subject(s)
Amyloid beta-Peptides/toxicity , Anti-Inflammatory Agents/therapeutic use , Inflammation/drug therapy , Lipopolysaccharides/toxicity , NF-kappa B/metabolism , Peptide Fragments/toxicity , Signal Transduction/drug effects , Sirolimus/therapeutic use , Animals , Brain/drug effects , Brain/pathology , Cell Line, Tumor , Cell Proliferation/drug effects , Cytokines/metabolism , Disease Models, Animal , Inflammation/chemically induced , Mice , Mice, Inbred C57BL , NF-kappa B/genetics , Neuroblastoma/pathology , Neuroglia/drug effects , TransfectionABSTRACT
Reports on the clinical entity of C1q nephropathy have focused on older children and young adult, data on old people are rare. In this report, we would introduce a 77-year-old woman who was diagnosed as C1q nephropathy by means of electron microscopic and immunofluorescence examination. Facial and lower extremity edema was the main reason for her to go for medical treatment, and she developed into acute renal failure within 5 d. Complete remission was observed after hemodialysis and steroid drugs treatments.
Subject(s)
Acute Kidney Injury/etiology , Complement C1q/metabolism , Acute Kidney Injury/metabolism , Acute Kidney Injury/therapy , Aged , Female , Glucocorticoids/therapeutic use , Humans , Prednisolone/therapeutic use , Renal DialysisABSTRACT
A neural stem cell line (RNSC-FMU 1) from rat brain have been established successfully by isolating and culturing neural stem cells from newborn SD rat brain in vitro with free-serum medium and passaging with mechanical division. The cell line cultured can continuously generate in vitro for long-term and it is 21 months (>100 passages) so far. These cells keep the feature of neural stem cell and normal karyotype. These neural stem cells can be induced to differentiate into neurons, astrocytes and oligodendrocytes. The cells have an extensive self-renewal capacity; its doubling time of proliferation is about 20 h. The cells are also cryopreservable. Tumor formation is not observed in nude mice that explanted with the cells. This cell line is a good tool for research of neural stem cell.
Subject(s)
Brain/cytology , Neurons/cytology , Stem Cells/cytology , Animals , Animals, Newborn , Astrocytes/cytology , Cells, Cultured , Cryopreservation , Mice , Mice, Inbred BALB C , Mice, Nude , Oligodendroglia/cytology , Rats , Rats, Sprague-Dawley , Stem Cell Transplantation/methodsABSTRACT
It is found that the distribution of Human Y chromosome relative length (Y/F) is different obviously within different ethnic and population. There are disputations on the relationship of Y/F with height and reproduction. By measuring the Y/F and height of 106 case (53 pairs of fathers and sons) of Fujian she ethnic, we can find that the Y chromosome relative length of Fujian she ethnic has plumb inheritance within pedigree and the appearance rate of long Y is 11.1%, below the medium level.
