ABSTRACT
AIM: To study the antioxidant effect of Ganoderma polysaccharide peptide (GLPP) and its mechanism. METHODS: Copper was used as oxidant to induce low lipoprotein (LDL) oxidative modification, and alloxan was given i.v. to induce reactive oxygen species (ROS) injury in mice. RESULTS: GLPP decreased oxidation of LDL and the relative electrophoretic mobility (REM) of oxidative product of LDL. After GLPP was given i.p. for 20 days, the concentration of malondialdehyde(MDA) in serum and heart of mice was decreased. The GSHpx enzyme activity was increased, while the SOD level was decreased. The catalase(CAT) levels were not significantly changed by GLPP. CONCLUSION: GLPP showed antioxidant effect by scavenging ROS or enhancing the enzyme activity of GSHpx in vivo and in vitro.
Subject(s)
Antioxidants/pharmacology , Glutathione Peroxidase/metabolism , Proteoglycans/pharmacology , Reishi/chemistry , Animals , Antioxidants/isolation & purification , Lipoproteins, LDL/metabolism , Male , Malondialdehyde/blood , Malondialdehyde/metabolism , Mice , Myocardium/metabolism , Oxidation-Reduction/drug effects , Plants, Medicinal/chemistry , Proteoglycans/isolation & purification , Random Allocation , Superoxide Dismutase/metabolismABSTRACT
AIM: To study the protective effects of Ganoderma lucidum polysaccharides peptide (GLPP) on the mice peritoneal macrophages injured by reactive oxygen species (ROS), derived from tert-butylhydroperoxide (tBOOH) in vitro and in vivo. METHODS: Mice peritoneal macrophages were injured by ROS, derived from tBOOH. The survival rate of macrophages was measured by MTT assay, and the morphological changes of macrophages were observed under light and electron microscopes. RESULTS: GLPP (50, 100, 200 mg/kg, ip for 5 d) could inhibit the foam cell formation and necrosis of macrophages. The survival rate of macrophages was increased. GLPP (3.125, 12.5, 50, 200 mg/L) given to the cultured macrophages brought the same protective effects. Under the electron microscope it was found that GLPP (100 mg/kg, ip, for 5 d) could protect the organelle such as mitochondria against injury by tBOOH. CONCLUSION: GLPP had significant scavenging ROS and antioxidant effects.
