ABSTRACT
In this study, a new porous beta-tricalcium phosphate (ß-TCP) granule was fabricated using the fibrous monolithic (FM) process and its in vitro biocompatibility and in vivo bone formation were evaluated. SEM micrograph images showed that MG-63 cells attached to the surfaces of the implant and were well proliferated. Cellular viability was as high as 75% in a50% extract dilution solution. cDNA micro array analysis was also carried out. In this analysis, we found a total of 12 up-regulated and 25 down-regulated genes. Four rabbits were used in the in vivo experiments. 3D micro-CT images showed that the formation of new bone was almost three times greater than that of normal trabecular bone (BV/TV). The histomorphometric results correlated with the micro-CT findings; a greater amount of new bone formation and osteoblast lineage along with osteocytes were observed in the implanted animals. Also x-ray radiographic and 2D micro-CT images were taken to demonstrate the superior biodegradability of the porous granule. As biodegradation occurred along with bone formation, 6 months after implantation, the porous granule structure was not distinguishable separately from that of the trabecular bone.
Subject(s)
Bone Substitutes/administration & dosage , Bone Substitutes/chemical synthesis , Calcium Phosphates/administration & dosage , Calcium Phosphates/chemical synthesis , Osteoblasts/physiology , Tissue Scaffolds , Animals , Cell Line , Cell Proliferation/drug effects , Cell Proliferation/physiology , Humans , Male , Materials Testing , Nanofibers/administration & dosage , Nanofibers/chemistry , Osteoblasts/cytology , Osteoblasts/drug effects , Osteogenesis/drug effects , Osteogenesis/physiology , Porosity , RabbitsABSTRACT
Synthesis and characterization of material properties of biphasic calcium phosphates (BCP)/polycaprolactone (PCL) composites, which were obtained by melt infiltration of PCL using porous BCP bodies, were investigated. Using 70 vol.% of poly methyl methacrylate (PMMA) powder as a pore-forming agent, porous BCP bodies were obtained by pressure less sintering depending on the temperature. The porous bodies obtained showed interconnected, spherical pores about 200 microm in diameter. Densification of the pore frame improved and grain growth increased remarkably as the sintering temperature increased. Molten PCL was infiltrated into porous BCP bodies to obtain the BCP/PCL composites. The material properties such as the relative density, hardness, bending strength, and elastic modulus of BCP/PCL composite, which was sintered at 1200 degrees C, were 95.7%, 11.2 Hv, 31.6 MPa and 10.2 GPa, respectively.
