The Effects of CCl4 on the Expressions of Aquaporin and Superoxide Dismutase in the Kidney of the Spontaneously Hypertensive Rat / 체질인류학회지

Carbon tetrachloride (CCl4) induces hepatocellular damage, resulting in liver cirrhosis by generating reactive oxygen species (ROS). At the stage of decompensated liver cirrhosis, many patients suffer from the abnormal regulation of sodium and water balance such as ascites. Also, the kidney can be directly damaged by CClCCl4-induced ROS generation. The aquaporin (AQP) is an important transmembrane protein located in the kidney to reabsorb water, and it may be affected by the ROS to alter water balance. ROS is related with the development of hypertension and alteration of antioxidant enzymes. This study was undertaken to investigate the effects of CCl4 on the expression of AQPs (AQP1 and AQP2) and superoxide dismutase (SOD) in the kidney of spontaneously hypertensive rat (SHR) and normotensive Wistar-Kyoto rat (WKY). The SOD is known as a scavenger of ROS, and we hypothesized that oxidative stress in the aged kidneys may be increased by hypertension. Male WKY and SHR were randomly divided into control and CCl4-treated groups at 8, 16 and 24 weeks of age, respectively. The experimental group received olive oil-dissolved CCl4 (1.6 mL/kg) on its back subcutaneous tissue twice a week for 4 weeks, and control animals received olive oil only. After 24 hours following the last injection, blood samples and kidneys were obtained under anesthesia. Renal histopathology was examined by H&E stain, and the expression of AQP1, AQP2, CuZn-SOD and Mn-SOD were evaluated by immunohistochemical methods and Western blot analysis. CCl4 treatment induced the tubular swelling, tubular epithelial atrophy or detachment in both WKY and SHR, and interstitial edema, tubular cast and infiltration of leukocyte in SHR. The BUN levels in both WKY and SHR were increased by CCl4 treatment at 16 weeks of age. The expression of AQP1 were increased by CCl4 treatment at 8 and 24 weeks of age SHR. The expressions of AQP2 in 24 week-old control SHR was decreased compared to 8 week-old control SHR. CCl4 treatment increased the expressions of AQP2 at 8 week-old and 24 week-old SHR, and the increasing of AQP2 was more remakable in advanced age. The expressions of CuZnSOD and MnSOD were increased at 24 week-old control SHR compared to same aged WKY. Whereas the expression of CuZnSOD was increased by CCl4 treatment in 8 week-old SHR, the expression of both CuZnSOD and MnSOD were decreased by CCl4 treatment in 24 week-old SHR. In summary, CCl4-treated SHR showed an increase in AQP expression and a decrease in SODs at the advanced age. These results suggest that CCl4-induced oxidative stress in the aged hypertensive rats may alter water balance via upregulation of AQPs and accelerate renal damage via downregulation of SODs.

A Morphometric Study on the Mandibular Foramen and the Lingula in Korean / 체질인류학회지

Interest in the correction of jaw deformities has increased due to the development of techniques and instruments of jaw operation. The accurate position of the mandibular foramen and lingula is important for the prevention of the complications and successful inferior alveolar nerve block. This study was conducted on 104 dried mandibles (65 males, 39 females) from Korean cadaver. The positions of the mandibular foramen and lingula were measured on the coronoid notch, occlusal plane, mandibular notch, gonion, koronoid and kondylion. The structures of the mandibular foramen and lingula were determined and the thickness of ramus and the angle of mandible were measured. Independent t-tests and paired t-tests were performed to determine the statistically significant difference. The results as follows; The line between coronoid notch and posterior border of ramus was located on the same level with the tip of lingula in 42.2% of the cases, and in between the mandibular foramen and the tip of lingula in 33.8% of the cases. The occlusal plane was located on the level between the mandibular foramen and the tip of lingula in 47.7% of cases, and on the same level with the tip of lingula in 35.6% of the cases. The line between lower end of the mandibular notch to inferior border of the mandible, in parallel to the posterior border of the mandibles, met the tip of lingula in 55.8% of the cases. The mandibular foramen was located posteriorly to the midpoint on the anteroposterior (AP) width of the ramus. It was located at 57.3% of AP width from the coronoid notch and 56.5% from the occlusal plane. The mandibular foramen and the tip of lingula were located superiorly to the midpoint on the vertical height of the ramus, on the 48.5% and 35.7% of vertical distance from the coronoid notch, respectively. The mandibular foramen was located at 40% of the length on the line from the gonion to the koronion. The results of this study will provide important morphometric information to prevent the complications in mandibular surgery and also provide the basis for the development of the new techniques.

Changes in HO-1, HSP70 and iNOS Expressions in the Rat Liver after Remote Ischemic Preconditioning / 체질인류학회지

Ischemic preconditioning (IP), short pre-treatment sublethal ischemia, induces a state of protection against subsequent prolonged ischemia-reperfusion. The purpose of this study was to investigate the expression of HO-1, HSP70, and iNOS proteins in the liver subjected to the courses of reperfusion after repetitive cycles of remote IP in the rat. Using thirty five week-old rats, the remote preconditioning was undertaken by vascular clamp occlusion of blood flow to one hindlimb, with 3 and 10 cycles of 5 minutes occlusion followed by 5 minutes reperfusion. The liver was removed 0, 3, 6, 24, and 72 hours of reperfusion after remote IP and assayed by immunohistochemical staining and Western blotting analyses for anti-HO-1, anti-HSP70, and anti-iNOS antibodies. The expression of HO-1 in rat liver increased at 72 hours of reperfusion groups after 3 and 10 cycles of remote IP, compared with normal control groups. The expression of HSP70 in rat liver increased at 6 hours of reperfusion groups after 3 cycles of remote IP, compared with normal control groups. The expression of HSP70 in rat liver increased at 0 hour of reperfusion groups after 10 cycles of remote IP, compared with normal control groups and decreased at 24 and 72 hours of reperfusion groups. The expression of iNOS in rat liver increased at 24 hours of reperfusion groups, but decreased at 72 hours of reperfusion groups after 3 and 10 cycles of remote IP, compared with normal control groups. In summary, these results showed that at early phase of reperfusion after remote IP, HSP70 expression was increased in rat liver. However, at 72 hrs of reperfusion after remote IP, HO-1 expression was increased and iNOS expression was decreased in rat liver.

TEM Observation on the Weathering Human Scalp Hair after Burial / 체질인류학회지

This study was undertaken to investigate the morphological changes of scalp hair according to weathering after burial and to offer basic data for forensic science. To examine the effect of weathering degree, the 51 human scalp hairs buried after death were taken from deceased individuals in tombs in Korea. The morphological changes in weathering hair shafts of the scalp were investigated using the transmission electron microscopes (TEM). There were little morphological differences of weathering depending on the burial period. The weathering changes includes detachment of cuticle layer, aggregation of macrofibril and hollow formation in the cortex according to the burial period. These results could provide basic data to the field of forensic medicine.

TEM Observation on the Weathering Human Scalp Hair after Burial / 체질인류학회지

This study was undertaken to investigate the morphological changes of scalp hair according to weathering after burial and to offer basic data for forensic science. To examine the effect of weathering degree, the 51 human scalp hairs buried after death were taken from deceased individuals in tombs in Korea. The morphological changes in weathering hair shafts of the scalp were investigated using the transmission electron microscopes (TEM). There were little morphological differences of weathering depending on the burial period. The weathering changes includes detachment of cuticle layer, aggregation of macrofibril and hollow formation in the cortex according to the burial period. These results could provide basic data to the field of forensic medicine.

Morphometrics of the Medial Longitudinal Arch of Foot in Korean / 체질인류학회지

In recent times the modification and injury of foot are shown due to various activity. It is essential to have detailed knowledges about the anatomical structure of medial longitudinal arch of foot for doing orthodigita or making shoes to prevent variation of medial longitudinal arch of foot. This study aimed to measure the constitution and to suggest the index of medial longitudinal arch of foot. Fifty four feet (30 M/24 F) obtained from the collection of the Department of Anatomy and Cell Biology of College of Medicine, Hanyang University were studied. We measured the length of foot, the height of foot, and the first ray angle. Also, we found the index of medial longitudinal arch and compared this results with various races anthropologically. Each items was analyzed using SPSS win 13.0. The length of foot was 211.12+/-13.02 mm and the height of foot was 39.03+/-6.27 mm. There was statistically significant difference between the values of male and female and among the age groups (p>0.001). The first ray angle was 24.98+/-2.16 degrees. There was statistically significant difference between the values of male and female (p>0.001). The medial longitudinal arch index was 18.43+/-2.33. The date shows clear distinction between the value of index in this study and the American's value of index as a result of comparing races. The results of this study would be useful to clarify the characteristics of the medial longitudinal arch.

Correlations between the Thickness of the Mandible and the Bone Mineral Density according to the Dentition / 체질인류학회지

The morphometric data which reflect the quality and quantity of the mandible are very important to the surgeon for the mandible. It is very useful clinically to predict the status of the mandible indirectly by other medical examinations. This study was undertaken to clarify the correlations of the mandible and dentition to predict the thickness and bone mineral density of the mandible. Sixty-one mandibles (32 M/29 F, mean age: 66.72 years) obtained from the collection of the Department of Anatomy and Cell Biology of Hanyang Medical College were analyzed. The bone mineral density was measured by dual energy X-ray absorptiometry. In the mandible, bone mineral densities at the mandiblular angle, between molars and premolars, around incisors and the thickness, and the length at the sections through the 1st and the 2nd molars were measured. The data were analysed with SPSS 12.0 program (One-Way ANOVA) according to age, gender and dentition, to verify the statistical significance and the correlation between the thickness and bone mineral densities. The obtained results were as follows. 1. The bone mineral density in the mandibular areas were variable, but statistically insignificant except incisor area. The bone density of the mandible was highly correlated with the thickness of cortical bones and the highest correlation coefficient was shown in sum of the thickness of buccal and lingual cortical bone (correlation coefficient, r=0.622) 2. The thickness of cortical bones at the sections through the molars in the man were greater than those in the woman. The correlation coefficient between the thickness and bone mineral density at the molars were greater in the woman. 3. The bone mineral density of whole mandible, the thickness of lingual and basal cortical bones and the height of alveolar ridge were significantly higher in the specimens with both of the 1st and 2nd mandibular molar teeth. The results of this study represents the significant differences of bone mineral density in the mandible according to gender and dentition and also the significant correlation between the bone mineral density and the thickness of cortical bone.

Microscopic Observation of Decomposition-inhibition Effect in GSE (Grapefruit Seed Extract) in Rat Liver / 체질인류학회지

Regarding to preserve the cadaver, formaldehyde has been used as a major preservative. However, the usage of formaldehyde has been considered by its harmful effects such as the disturbing ordor, toxicities and limitations to use. Therefore we studied the effect of decomposition-inhibition which is a natural product, grapefruit seed extract (GSE). Concerning the preservative activity, we sacrificed 8 week old male SD rat and collected liver. Using liver tissues, we treated GSE as a time dependant manner under 37degrees, 80+/-5% humidity conditions. To confirm GSE effects, we applied light and electron microscopic analysis. In results, we observed GSE attenuated the morphological changes and putrefaction of liver tissues more than 3 days. Herein, we introduced the potential substitute of formaldehyde to preserve the cadaver as well as animal tissues.

Morphometrics of the Metatarsal Bone and Regression Equation of the Metatarsal Length in Korean / 체질인류학회지

As the interest in health is increasing and the population enjoyed the leisure sports is steadily increasing, the stress fracture, fracture or variant of metatarsal bone of foot has been shown frequently. The mistaken estimation about the length and rank of metatarsal bones during the osteotomy of the metatarsal bones of foot can be complicated. It is essential to have detailed knowledges about the anatomical structure of surgical region. This study aimed to investigate the metatarsal bones of foot and to develop a regression equation that can predict the length of metatarsal bones during the osteotomy. The subject of this study is fifty four feet (30M/224F). We measured the whole length and the article length of metatarsal bone. Also, we measured the whole width and the article width in the head, body, base of the metatarsal bone. The data was analyzed using SPSS win 13.0. The regression equation models of length of the metatarsal bones were developed by multiple regression analysis. The regression equation predicted first metatarsal length was second metatarsal articular length x0.770+7.780, second metatarsal length was third metatarsal length x0.976+6.050, third metatarsal length was fourth metatarsal length x1.000+0.922, fourth metatarsal length was third metatarsal length x0.917+4.167, fifth metatarsal length was fourth metatarsal length x0.901+7.972. The results of this study would be useful to clarify the characteristics of the metatarsal bone of the foot, to develop a regression equation for prediction of the length of the metatarsal bone.

Correlations of Bone Mineral Density between Mandible and Vertebra, Forearm, and Femur / 체질인류학회지

Generally, osteoporosis is caused by the age-related physiologic bone loss. In cases of severe osteoporosis, the reduced bone mass and increaed skeletal fragility make the risk of fracture high. Recently, the relationship between the mandible and the condition of the skeletal bone, the molphometric data about the quality and quantity of the mandible are very important in the diagnosis and treatment of osteoporosis. This study was undertaken to clarify the correlations of the mineral density between mandible and other bones, to predict the bone mineral density (BMD) of the mandible. Cervical (2nd~5th) and lumbar (1st~4th) vertebrae, the bones of forearm and proximal femurs, mandibles from 42-embalmed cadavers (22/M, 20/F, mean age: 66.95 years) were examined. The BMD was measured by dual energy X-ray absorptiometry. The data were analysed with SPSS 12.0 program according to age, gender, to verify the statistical significance. 1. The BMD in the mandibular areas were variable. The BMD in the angle of the mandible (mean 0.40 g/cm2) was similar with that of ultra-distal part of the forearm (mean 0.34 g/cm2). 2. The BMD in the mandible was highly correlated with that of around the forearm. The correlation coefficient between density in the angle, the part between 1st and 2nd molar, the part between 1st molar and 2nd premolar and incisor portion of the mandible and density in the 1/3-distal part of the forearm was high (r=0.742, 0.697, 0.618, 0.652). The correlation coefficient between density in the part between 1st and 2nd premolar and density in the total femur was high (r=0.542). 3. The BMD in the mandible was correlated with that of around the femur neck (r=0.607). 4. The correlation coefficient between the BMD in the 1st and 2nd molar of the mandible and the BMD in the 5th cervical vertebra was high (r=0.543). And also, the correlation coefficient between the BMD in the incisor area of the mandible and the BMD in the 3rd lumbar vertebra was high (r=0.561). 5. Bony status of the mandible was classified into normal, osteopenia, osteoporosis by BMD of WHO criteria. The data represented that BMD of the mandible was decreased as BMD of the skeletal bones decreased. It is concluded that it could be possible to predict BMD of the mandible by the measurement of BMD in the forearm.

Expressions of iNOS and Superoxide Dismutase in the Skeletal Muscle of the Spontaneously Hypertensive Rat after Ischemic-preconditioning / 체질인류학회지

A balance between production and degradation of reactive oxygen species has an important role in the cardiovascular homeostasis, and is known to contribute to hypertension. Under oxidative stress, an upregulation of inducible NOS (iNOS) induces ischemic-reperfusion injury, and is involved in the pathophysiology of the hypertension. Ischemic-reperfusion injury of the skeletal muscle results from reactive oxygen species, and overexpression of iNOS in the skeletal muscle increases the ischemic injury. Superoxide dismutase (SOD), antioxidant, is a major enzyme for degradation of reactive oxygen species (ROS). The purpose of this study was to observe the effect ischemic preconditioning (IP) of the lower limb on the expression of iNOS, CuZnSOD and MnSOD in the white and red muscle of the spontaneously hypertensive rat (SHR). Nine weeks old male normotensive rat (Wistar-Kyoto rat, WKY) and SHR were divided into control and IP groups. The IP group was further divided into 3 (3IP) and 10 (10IP) times of IP. Left common iliac artery was occluded 3 and 10 times for 5 min of ischemia-5 min of reperfusion using rodent vascular clamp. The animals were sacrificed at 0, 0.5, 1, and 3 hours after reperfusion and the Tibialis anterior and Soleus were removed. The expressions of iNOS, CuZnSOD and MnSOD in the skeletal muscle were examined with immunohistochemical methods and Western blot analysis. iNOS was expressed in Tibialis anterior, but in Soleus after IP. The expression of iNOS was increased in both WKY and SHR, it was higher in SHR than WKY. CuZnSOD and MnSOD were expressed in Tibialis anterior and Soleus, higher in Soleus, after IP. The expression of CuZnSOD and MnSOD were increased in both WKY and SHR, higher in WKY than SHR. It is consequently suggested that hypertensive individual and white muscle are more sensitive to ischemic injury of the skeletal muscle as considering their high expression of iNOS and low expression of SODs.

Effects of Ischemic Preconditioning on the Phosphorylation of Akt and the Expression of SOD-1 in the Ischemic-reperfused Rat Skeletal Muscles / 체질인류학회지

Akt, a key protein of cell survival, can promote cell growth and survival by activations of various cellular protective factors. Ischemic preconditioning (IP) has been known to reduce ischemic injury through upregulation of phosphorylation of Akt (p-Akt). CuZn-superoxide dismutase (SOD-1), an antioxidant enzyme, scavenges reactive oxygen species and protects cell from oxidative stress by increasing the activaiton of Akt. The present study was performed to examine the effects of IP on the expression of p-Akt and SOD-1 in the ischemicreperfused rat skeletal muscles. Thirty weeks old male SD rats were divided into 4 groups, such as controls, IP, 4 hour ischemia and 4 hour ischemia with IP. For IP, commom iliac artery was occluded three times for 5 min ischemia followed by 5 min reperfusion using rodent vascular clamps. Ischemia was induced by occlusion on the same artery for 4 hours. The Tibialis anterior and Soleus were removed at 0, 1, 3, and 24 hours of reperfusion. The expressions of p-Akt (Ser 473) and SOD-1 were examined with immunohistochemistry and Western blot analysis.In the IP group, the p-Akt and SOD-1 were increased, compared to the control group. In the ischemia group, the p- Akt and SOD-1 were decreased, compared to the control group, and were more abundant when reperfusion time were increased. IP increased the p-Akt and SOD-1 after 4 hour ischemia, and the p-Akt and SOD-1 were higher in Soleus compared to Tibialis anterior. These findings suggest that IP increases p-Akt and expression of SOD-1 in the ischemic-reperfused rat skeletal muscles, and that upregulations of p-Akt and SOD-1 induced by IP were higher in the red muscle fiber, Soleus, than the white muscle fiber, Tibialis anterior.

Regressive Equation of the Maxillary Sinus / 체질인류학회지

It is important that the surgeons expect difference in the volume of the maxillary sinus during endoscopic surgery because a variation in volume of the maxillary sinus is related to a variation in anatomical landmarks. Forty four Korean skulls (88 sinuses, 23 M/21F) were used in this study. Anatomical landmarks around the maxillary sinus were measured. The significant variables (r.0.03, P.0.05) out of measured variables were pick out by use of SPSS-13.0 program. The regression equation models were developed by multiple regression analysis. The volume of maxillary sinus showed a high correlation with 19 variables included the width, height, depth of the maxillary sinus, depth on C-arm radiography, length of three borders of maxillary sinus, distance between the lowest point of orbit and the deepest point of maxillary sinus, distance between the deepest point of maxillary sinus and the porion, width of orbit, measured values from akanthion. The regression equation model for prediction of the volume of the maxillary sinus was volume=0.692?(the anteroposterior length of the maxillary sinus)+0.355?(the height of the maxillary sinus)-22.178. This study ascertained variables of a high correlation with volume of the maxillary sinus and would be useful to develop the regression model for prediction of volume of the maxillary sinus.

Effects of Remote Ischemic Preconditioning on the Expressions of NOS and Akt in the Rat Myocardium / 체질인류학회지

Remote ischemic preconditioning (IP), brief tolerating cycles of ischemia and reperfusion in remote non-vital organs, can reduce ischemic injury of the heart. IP induces cardiac protection by down-regulating iNOS or up-regulating eNOS. In addition, Akt has been known to protect myocardium against ischemia-reperfusion injury. This study was undertaken to observe the expression of iNOS, eNOS, Akt and phospho-Akt (p-Akt) in the rat myocardium after IP. Thirty-five weeks-old male Sprague-Dawley rats were divided into control and IP groups. The IP group was further subdivided into 3 groups based on the number of cycles of IP. For IP, left commom iliac artery was occluded 3, 6 and 10 cycles for 5 min of ischemia alternating with 5 min of reperfusion. The rat were sacrificed at 0, 3, 6, 24 and 72 hours of IP and the heart was removed. The expression of iNOS, eNOS, Akt and p-Akt in the rat myocardium was examined by immunohistochemical staining and Western blot analysis. The expression of iNOS was increased by IP and was higher in 10IP groups than 3IP and 6IP group. The expression of eNOS was increased or decreased by IP and was showed no difference with increasing episode of IP. The expression of Akt was decreased by IP at 24 and 72 hours after reperfusion, and showed no differences with increasing episode of IP. The expression of p-Akt was increased by IP and showed no difference with increasing episode of IP. These results suggest that hind limb ischemic preconditioning provides cardiac protection through up-regulation of eNOS and phosphorylation of Akt, however excessive episodes of remote preconditioning may induce the myocardial ischemic injury through overexpression of iNOS.

Expressions of Nitric Oxide Synthases and Akt in the Tibialis Anterior Muscle of the Spontaneously Hypertensive Rat after Ischemic Preconditioning / 체질인류학회지

Nitric oxide (NO) generated during ischemic-reperfusion phase is known to activate various transcriptional factors. NO is generated by 3 nitric oxide synthase (NOS) isoforms, nNOS, iNOS, eNOS. Akt plays a important role of the cell survival. Ischemic preconditioning (IP) reduces ischemic injury by changing of the expression of the NOSs and Akt. The alterations of the NOSs is related to the development of hypertension, and hypertension has been known to induce the skeletal muscle changes including decreased fatigue resistance and contractile force. The purpose of this study was to observe the effect of IP on the expressions of the NOSs and Akt in the skeletal muscle of the spontaneously hypertensive rat (SHR). Nine weeks old male normotensive rat (Wistar-Kyoto rat, WKY) and SHR were divided into control and IP groups. The IP group was further divided into 3 or 10 cycles of IP. For IP, left common iliac artery was occluded 3 or 10 cycles of 5 min of ischemia/5 min of reperfusion using rodent vascular clamp. The animals were sacrificed at 0, 0.5, 1, 3 and 24 hours after reperfusion and the tibialis anterior muscles were removed. The expressions of nNOS, iNOS, eNOS, and Akt were examined with immunohistochemical methods and Western blot analysis. In the SHR, the expression of nNOS was decreased, iNOS was increased and the expressions of eNOS and AKT were decreased, compare to WKY. The expression of iNOS was increased, and nNOS, eNOS and Akt were decreased in SHR by 10IP. In summary, IP increased the expression of iNOS, and decreased the expression of eNOS and Akt in the SHR. It is suggested that hypertension may aggravate injury of the tibialis anterior muscle induced by repetitive ischemic conditioning.

The Position of Mandibular Canal in the Sections of the Mandible / 체질인류학회지

The complications and sequelae after the mandibular surgery are related to inferior alveolar neurovascular bundles, which pass through the mandibular canal. The knowledge of their positions would decrease the risk of mandibular surgery dramatically and would be used for the development of the new surgical techniques. This study was undertaken to clarify the anatomical position of mandibular canal for the mandibular surgery. Forty four mandibules (23 males and 21 females average 66.5 years) obtained from the collection of Hanyang medical college were studied. The location of mandibular canal in the sections between premolars and molars were measured. The obtained results were as follows; At first, the mandibular canal lay lingual to the distal part of the body of the mandible. It then ran anteriorly and to the buccal part of the mandible between the first and the second premolars. In the sections between premolars and molars, the distance between the mandibular canal and the lower border of mandibular body was 8.9+/-1.9 mm at the position of the first molar, the distance between the deepest point of the alveolar socket and the mandibular canal was 9.5+/-3.5 mm at the second molar, which was the narrowest point. The results of this study would be useful to decrease the risk of the mandibular surgery and to develop the new techniques for mandibul surgery in the field of the dentistry and maxillofacial surgery.

Effects of Repetitive Ischemic Preconditioning on the Expression of Nitric Oxide Synthase in Tibialis Anterior and Soleus Muscles of the Rat / 체질인류학회지

Nitric oxide synthases (NOSs) that catalyzed the conversion of L-arginine to nitric oxide and L-citrulline play a role in ischemic-reperfusion injury. The purpose of this study was to observe the expression patterns of nNOS, iNOS and eNOS in the rat tibialis anterior and soleus muscles after multiple cyclic episodes of ischemic preconditioning (IP). Nine weeks old male SD rats were divided into control and IP groups. The IP group was further divided into 3 groups based on cycle of IP. For IP, left commom iliac artery was occluded 3, 6 and 10 times for 5 minutes ischemia followed by 5 minutes reperfusion using rodent vascular clamps. The animals were sacrificed at 0, 3, 6, 24 and 72 hours of reperfusion and the left tibialis anterior and soleus muscles were removed. The expression of nNOS, iNOS and eNOS were examined with immunohistochemical methods and Western blot analysis. IP increased the expression of nNOS, compared with the control. In the tibialis anterior muscle, the levels of nNOS in the 3IP and 6IP were higher than that in 10IP. IP increased the expression of iNOS, compared with the control, and the levels of iNOS in tibialis anterior muscle were higher than that in soleus muscle. The level of iNOS in the 10IP was higher than those in the 3IP and 6IP. IP increased the expression of eNOS, compared with the control, and the level of eNOS in soleus muscle were higher than that in tibialis anterior muscle. At 0 and 3 hours after reperfusion, the level of eNOS in 6IP and 10IP were higer than that in 3IP. In summary, these results suggest that the ischemic preconditioning increases the expression of nNOS, iNOS and eNOS, and 10 times of ischemic preconditioning may induce ischemic injury through upregulation of iNOS. And tibialis anterior muscle is more susceptabile to ischemic injury than soleus muscle.

Morphometry of the Korean Nasal Turbinates / 체질인류학회지

Recently, with the increasing interests in the endoscopic nasal surgery, it is essential for practitioners to have detailed knowledges of the anatomical structures for the successful surgery. This study was undertaken to provide morphological information of the nasal turbinates as anatomical reference during endoscopic nasal surgery. A total of 43 adult cadaver heads were bisected midsagitally and evaluated morphometrically. The results were as follows: The highest nasal turbinatee were observed in 66.7% of the case. After removal of the mucosa, the distances from the akanthion to the anterior end of superior, middle, inferior nasal turbinate were 46.75 mm, 38.23 mm, 18.51 mm respectively. The distances from the akanthion to the posterior end of superior, middle, inferior nasal turbinate were 55.11 mm, 54.72 mm, 50.26 mm respectively. The lengths of the superior, middle, inferior nasal turbinate were 22.20 mm, 40.14 mm, 40.97 mm respectively. The detailed measurements of the nasal turbinates performed in this study may provide useful anatomical landmarks for the endonasal endoscopic surgery.

The Morphology and Anatomical Location of the Sphenopalatine Foramen / 체질인류학회지

This study aims to investigate the anatomical information of the sphenopalatine foramen with special reference to the bleeding control of the sphenopalatine artery and to the blockage of the pterygopalatine ganglion during functional endoscopic sinus surgery. Forty-three midsagittal sectioned Korean heads were used in the study. The mucosa on the lateral nasal wall was removed for showing the sphenopalatine foramen. The shapes of the sphenopalatine foramen were classified by 4 types. Angle from the akanthion and distances from akanthion, conchae, sphenoidal sinus and greater palatine foramen were measured. The sphenopalatine foramen was frequently found as oval shape and bilateral symmetry of 28.6%. The vertical length was 5.5 mm, the horizontal length was 5.3 mm. The most anterior point of the sphenopalatine foramen was located on 54.4 mm few and angles were between 15.9degrees and 22.1degrees from akanthion. The distances were 36.2 mm from anterior end of middle nasal concha, 6.2 mm from posterior end of middle nasal concha, 27.2 mm from greater palatine foramen. The uppermost point of the sphenopalatine foramen was located on 22.3 mm from the most superoanterior end of the sphenoidal sinus, 18.6 mm from the lowermost end of the sphenoidal sinus. This study could be useful to provide the confidence about the location of the sphenopalatine foramen during functional endoscopic sinus surgery.

Alterations of PKC theta, NF-kappa B, and AP-1 in Ischemic-reperfused Tibialis Anterior and Soleus Muscles of Rats / 체질인류학회지

Ischemic-reperfusion injury of skeletal muscles takes place in the phase of reperfusion and induces cellular damages through activating various transcription factors and genes, which initiate signal transduction. The purpose of this study was to observe changes of expression of NF-kappa B and AP-1, which are known as a redox sensitive transcription factors in ischemic-reperfused rat skeletal muscles, and PKC theta which activate NF-kappa B and AP-1. Sprague-Dawley male rats of nine, thirty, and sixty-five weeks old were divided into control and ischemia groups. Ischemia was performed by occlusion of left common iliac artery for 4 hours using rodent vascular clamps. The animals were sacrificed at hours 0, 1, 3 and 6 after onset of reperfusion and tibialis anterior and soleus muscles were removed. The distributions of PKC theta, NF-kappa B, and AP-1 immunoreactivity (IMR) were examined using immunohistochemical methods. The results as follows; In control groups, PKC theta IMR was decreased with age and was higher in tibialis anterior than that in soleus muscles. In ischemia groups, PKC theta IMR was increased with age and was higher in soleus than that in tibialis anterior muscles. In control groups, NF-kappa B IMR was decreased with age. In ischemia groups, NF-kappa B IMR was increased with age and was higher in soleus than that in tibialis anterior muscles. In control groups, AP-1 IMR was decreased with age. In ischemia groups, AP-1 IMR was increased with age and was higher in tibialis anterior than that in soleus muscles. Increase or decrease of PKC theta IMR was associated with the increase or decrease of NF-kappa B and AP-1 IMR in ischemic-reperfused rat skeletal muscles, respectively. These results suggested that the increased expression of PKC theta may induce the upregulations of NF-kappa B and AP-1 in ischemic-reperfusion injury of rat skeletal muscle. It is also suggested that the ischemic injury may be increased with age, and tibialis anterior muscle is more susceptabile to ischemic-reperfusion injury than soleus muscle.