Subject(s)
Brain Diseases/cerebrospinal fluid , Nervous System Diseases/cerebrospinal fluid , Psychotic Disorders/cerebrospinal fluid , Uric Acid/cerebrospinal fluid , Adult , Age Factors , Aged , Alcoholism/cerebrospinal fluid , Atrophy , Brain Diseases/diagnostic imaging , Female , Hospitalization , Humans , Male , Middle Aged , Nervous System Diseases/diagnostic imaging , Pneumoencephalography , Psychotic Disorders/diagnostic imagingSubject(s)
Brain Chemistry , Glycosaminoglycans/isolation & purification , Aging , Animals , Brain/growth & development , Brain/metabolism , Cats , Chondroitin/analysis , Female , Glycosaminoglycans/metabolism , Heparin/analysis , Hyaluronic Acid/analysis , Lipids/analysis , Male , Uronic Acids/analysis , Water/analysisSubject(s)
Brain/physiology , Cerebral Cortex/physiology , Motor Neurons , Spinal Cord/pathology , Spinal Nerves/physiology , Animals , Brain/surgery , Cats , Deoxyribonucleases/analysis , Deoxyribonucleases/metabolism , Histocytochemistry , Lysosomes/metabolism , Methods , Spinal Cord/enzymology , Spinal Nerves/surgeryABSTRACT
The average yield of mucopolysaccharides isolated from adult cat brain was 3.29 mg, representing 0.02 per cent of the wet wt. of brain tissue. The lipid-free dried brain was approximately 10 per cent of the wet brain tissue weight. Mucopolysaccharide fraction distributions and concentration ratios are presented. Chronic administration of testicular hyaluronidase to adult cats resulted in an advanced stage of neurological impairment, a decrease in hyaluronic acid to about 40 per cent of the control value, and an even slightly greater decrease in chondroitin sulphate. With limited hyaluronidase injections, neurological impairment was milder; mucopolysaccharide concentrations after four doses of hyaluronidase decreased to approximately the same level as in chronic experimental cats.