Experimental measurement and numerical modelling of dye washout for investigation of blood residence time in ventricular assist devices.

Ventricular assist devices have become the standard therapy for end-stage heart failure. However, their use is still associated with severe adverse events related to the damage done to the blood by fluid dynamic stresses. This damage relates to both the stress magnitude and the length of time the blood is exposed to that stress. We created a dye washout technique which combines experimental and numerical approaches to measure the washout times of ventricular assist devices. The technique was used to investigate washout characteristics of three commercially available and clinically used ventricular assist devices: the CentriMag, HVAD and HeartMate II. The time taken to reach 5% dye concentration at the outlet (T05) was used as an indicator of the total residence time. At a typical level of cardiac support, 5 L/min and 100 mmHg, T05 was 0.93, 0.28 and 0.16 s for CentriMag, HVAD and HeartMate II, respectively, and increased to 5.06, 1.64 and 0.96 s for reduced cardiac support of 1 L/min. Regional variations in washout characteristics are described in this article. While the volume of the flow domain plays a large role in the differences in T05 between the ventricular assist devices, after standardising for ventricular assist device volume, the secondary flow path was found to increase T05 by 35%. The results explain quantitatively, for the first time, why the CentriMag, which exerts low shear stress magnitude, has still been found to cause acquired von Willebrand Syndrome in patients.

Effect of salts on water viscosity in narrow membrane pores.

The effect of various salts on the viscosity, and by implication structure, of water in polymeric membrane pores of radius approximately 1.69 nm and low charge density has been studied. Permeation of pure water and various electrolyte solutions was analyzed using the Hagen-Poiseuille equation expressed in a ratio form to exclude membrane-specific quantities such as pore radius and length. The analysis produced viscosity ratios of electrolyte to pure water inside the membrane pores. Comparing the viscosity ratios inside the pores with their bulk counterparts showed that confinement significantly increased the sensitivity of water structure to the presence of ions. It has been found that, in relative terms in the pores, Cl- was a strong structure breaker, K+ was a moderately strong structure breaker, Na+ was a weak structure breaker, SO4(2-) was a weak structure maker, and Mg2+ was a strong structure maker. Predictive modeling of membrane separation performance would benefit from such effects being taken into account in cases where the pore ion concentrations may be high.