ABSTRACT
A novel ligand dipyrido[1,2,5]oxadiazolo[3,4-b]quinoxaline (dpoq) and its complexes [Ru(bpy)(2)(dpoq)](2+) and [Ru(phen)(2)(dpoq)](2+) (bpy=2,2'-bipyridine; phen=1,10-phenanthroline) have been synthesized and characterized by elemental analysis, electrospray mass spectra and (1)H NMR. The interaction of Ru(II) complexes with calf thymus DNA (CT-DNA) was investigated by absorption spectroscopy, fluorescence spectroscopy, thermal denaturation and viscosity measurements. Results suggest that two Ru(II) complexes bind to DNA via an intercalative mode.
Subject(s)
DNA/chemistry , Pyridines/chemistry , Quinoxalines/chemistry , Ruthenium Compounds/chemistry , Binding, Competitive , Ligands , Nucleic Acid Denaturation , Pyridines/chemical synthesis , Quinoxalines/chemical synthesis , Ruthenium Compounds/chemical synthesis , Titrimetry , ViscosityABSTRACT
Two novel DNA-intercalating ruthenium(II) complexes, [Ru(bpy)(2)(PIPSH)](2+) and [Ru(bpy)(2)(PIPNH)](2+), have been synthesized and characterized. Gel retardation assay, atomic force microscopy, and dynamic light scattering studies show that both complexes can induce the condensation of originally circular plasmid pBR322 DNA to particulate structures under neutral conditions.
