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1.
Epidemiol Infect ; 148: e289, 2020 12 09.
Article in English | MEDLINE | ID: mdl-33292874

ABSTRACT

An acute gastroenteritis (AGE) outbreak caused by a norovirus occurred at a hospital in Shanghai, China, was studied for molecular epidemiology, host susceptibility and serological roles. Rectal and environmental swabs, paired serum samples and saliva specimens were collected. Pathogens were detected by real-time polymerase chain reaction and DNA sequencing. Histo-blood group antigens (HBGA) phenotypes of saliva samples and their binding to norovirus protruding proteins were determined by enzyme-linked immunosorbent assay. The HBGA-binding interfaces and the surrounding region were analysed by the MegAlign program of DNAstar 7.1. Twenty-seven individuals in two care units were attacked with AGE at attack rates of 9.02 and 11.68%. Eighteen (78.2%) symptomatic and five (38.4%) asymptomatic individuals were GII.6/b norovirus positive. Saliva-based HBGA phenotyping showed that all symptomatic and asymptomatic cases belonged to A, B, AB or O secretors. Only four (16.7%) out of the 24 tested serum samples showed low blockade activity against HBGA-norovirus binding at the acute phase, whereas 11 (45.8%) samples at the convalescence stage showed seroconversion of such blockade. Specific blockade antibody in the population played an essential role in this norovirus epidemic. A wide HBGA-binding spectrum of GII.6 supports a need for continuous health attention and surveillance in different settings.


Subject(s)
Caliciviridae Infections/virology , Gastroenteritis/epidemiology , Gastroenteritis/virology , Norovirus/classification , Adult , Aged , Antibodies, Viral/blood , Blood Group Antigens , Caliciviridae Infections/epidemiology , China/epidemiology , Cross Infection/epidemiology , Cross Infection/virology , Disease Outbreaks , Hospitals , Humans , Immunoglobulin G/blood , Male , Middle Aged , Norovirus/genetics , Phylogeny , Protein Binding
2.
Genet Mol Res ; 15(3)2016 Aug 26.
Article in English | MEDLINE | ID: mdl-27706608

ABSTRACT

The temporal and spatial patterns of Smad and Yes-associated protein 1 (YAP1) expression were investigated in skeletal muscle (gastrocnemius muscle and extensor digitorum longus) at different growth stages (2 days old, 2 and 6 months old) in Hu sheep. Smads were differentially expressed in sheep skeletal muscle, with high expression in the gastrocnemius muscle and lower expression in the extensor digitorum longus. Expression of Smad2, Smad3, and Smad4 at the 2-day-old stage was significantly higher than at other stages (P < 0.05). The expression of Smad7 in 2-day-old sheep was lower than in 6-month-old sheep, with the lowest levels at 2 months. Smad expression was higher in males than in females at the 2-day-old stage, and expression in 2- and 6-month-old males was lower than that in 2-day-old females. Smad3 expression was higher in the 2-day- and 2-month-old males than in the females. There was a positive correlation (P < 0.01) between YAP1 and Smad2 expression in gastrocnemius muscle at the 2-month-old stage. YAP1 and Smad4/7 expression were positively correlated (P < 0.01) in extensor digitorum longus at the 2-day-old stage. YAP1 expression was negatively correlated with Smad7 in the extensor digitorum longus at 6 months. A significant difference between Smad2 and Smad3 (P < 0.01) expression in muscle was observed, consistent with Smad3 and Smad4 expression, indicating that these inhibit transforming growth factor-ß signaling in the same way. There was a positive correlation (P < 0.01) between YAP1 and MSTN expression, suggesting that YAP1 participates in muscle growth in sheep.


Subject(s)
Adaptor Proteins, Signal Transducing/genetics , Aging/genetics , Muscle, Skeletal/metabolism , Smad2 Protein/genetics , Adaptor Proteins, Signal Transducing/metabolism , Aging/metabolism , Animals , Animals, Newborn , Female , Gene Expression Regulation, Developmental , Male , Muscle, Skeletal/growth & development , Myostatin/genetics , Myostatin/metabolism , Sheep , Sheep, Domestic , Signal Transduction , Smad2 Protein/metabolism , Smad3 Protein/genetics , Smad3 Protein/metabolism , Smad4 Protein/genetics , Smad4 Protein/metabolism , Smad7 Protein/genetics , Smad7 Protein/metabolism , Transforming Growth Factor beta/genetics , Transforming Growth Factor beta/metabolism
3.
Genet Mol Res ; 15(2)2016 May 23.
Article in English | MEDLINE | ID: mdl-27323083

ABSTRACT

The mRNA expression levels of key genes (Smads, MSTN, and MyoG) in the TGF-ß/Smad signaling pathway in Hu sheep at different growth stages (2 days, 2 months, and 6 months of age) and in different skeletal muscles (longissimus dorsi muscle and soleus muscle) and different genders were detected; and correlation of the Smad family (Smad2, Smad3, Smad4, and Smad7), MSTN, MyoG expressions was analyzed in Hu sheep. The results showed that the expression of Smads was higher in the soleus muscle than in the longissimus dorsi muscle; the expressions of Smad2, Smad3, and Smad4 were significantly higher in 2-day-old sheep than in sheep belonging to the other age groups (P < 0.05); the expressions of Smad2, Smad4, and Smad7 were higher in rams than in 2-day-old ewes, but lower in rams than in 2-month-old and 6-month-old ewes; and the expression of Smad3 was higher in rams than in 2-day-old and 2-month-old ewes, but lower in rams than in 6-month-old ewes. In the 2 different muscle tissues, expression of Smad2 was significantly positively correlated (P < 0.01) with that of Smad3. The expression of Smad3 was significantly positively correlated (P < 0.01) with that of Smad4, which showed that the Smad family genes could have an inhibitory effect on the TGF-ß/Smad signaling pathway.


Subject(s)
Sheep/genetics , Smad2 Protein/biosynthesis , Smad3 Protein/biosynthesis , Smad4 Protein/biosynthesis , Transforming Growth Factor beta/biosynthesis , Animals , Gene Expression Regulation, Developmental , Muscle Development/genetics , Muscles/metabolism , Sheep/growth & development , Signal Transduction/genetics , Smad2 Protein/genetics , Smad3 Protein/genetics , Smad4 Protein/genetics , Smad7 Protein/biosynthesis
4.
Br J Surg ; 102(8): 959-64, 2015 Jul.
Article in English | MEDLINE | ID: mdl-25980461

ABSTRACT

BACKGROUND: This study evaluated the clinical characteristics, surgical procedures and prognosis of duodenal gastrointestinal stromal tumours (GISTs). METHODS: Patients with a diagnosis of primary duodenal GIST treated between January 2000 and December 2012 were analysed. Patients with gastric and small intestinal GISTs were chosen as control groups according to the following parameters: age, tumour size, mitotic index and adjuvant imatinib therapy. Operative procedures for patients with duodenal GIST included pancreaticoduodenectomy or limited resection. Disease-free survival (DFS) was calculated using Kaplan-Meier analysis. RESULTS: Some 71 patients with duodenal, 71 with gastric and 70 with small intestinal GISTs were included in the study. DFS of patients with duodenal GIST was shorter than that of patients with gastric GIST (3-year DFS 84 versus 94 per cent; hazard ratio (HR) 3.67, 95 per cent c.i. 1.21 to 11.16; P = 0.014), but was similar to that of patients with small intestinal GIST (3-year DFS 84 versus 81 per cent; HR 0.75, 0.37 to 1.51; P = 0.491). Patients who underwent pancreaticoduodenectomy were older, and had larger tumours and a higher mitotic index than patients who had limited resection. The 3-year DFS was 93 per cent among patients who had limited resection compared with 64 per cent for those who underwent PD (HR 0.18, 0.06 to 0.59; P = 0.001). CONCLUSION: The prognosis of duodenal GISTs is similar to that of small intestinal GISTs.


Subject(s)
Duodenal Neoplasms/surgery , Gastrointestinal Stromal Tumors/surgery , Adult , Aged , Aged, 80 and over , Disease-Free Survival , Duodenal Neoplasms/pathology , Female , Gastrointestinal Stromal Tumors/mortality , Gastrointestinal Stromal Tumors/pathology , Humans , Intestinal Neoplasms/mortality , Intestinal Neoplasms/surgery , Intestine, Small/surgery , Male , Middle Aged , Pancreaticoduodenectomy , Prognosis , Stomach Neoplasms/mortality , Stomach Neoplasms/surgery , Survival Analysis
5.
Parasitology ; 139(8): 1029-37, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22444160

ABSTRACT

Cysteine proteases of pathogenic protozoan parasites play pivotal roles in the life cycle of parasites, but strict regulation of their activities is also essential for maintenance of parasite physiology and interaction with hosts. In this study, we identified and characterized cryptostatin, a novel inhibitor of cysteine protease (ICP) of Cryptosporidium parvum. Cryptostatin showed low sequence identity to other chagasin-family ICPs, but 3 motifs (NPTTG, GXGG, and RPW/F motifs), which are evolutionarily conserved in chagasin-family ICPs, were found in the sequence. The overall structure of cryptostatin consisted of 8 ß-strands that progressed in parallel and closely resembled the immunoglobulin fold. Recombinant cryptostatin inhibited various cysteine proteases, including papain, human cathepsin B, human cathepsin L, and cryptopain-1, with K i's in the picomolar range. Cryptostatin was active over a wide pH range and was highly stable under physiological conditions. The protein was thermostable and retained its inhibitory activity even after incubation at 95°C. Cryptostatin formed tight complexes with cysteine proteases, so the complexes remained intact in the presence of sodium dodecyl sulfate and ß-mercaptoethanol, but they were disassembled by boiling. An immunogold electron microscopy analysis demonstrated diffused localization of cryptostatin within oocystes and meronts, but not within trophozoites, which suggests a possible role for cryptostatin in host cell invasion by C. parvum.


Subject(s)
Cryptosporidium parvum/genetics , Cystatins/chemistry , Cysteine Proteases/metabolism , Cysteine Proteinase Inhibitors/chemistry , Protozoan Proteins/metabolism , Amino Acid Motifs , Cathepsin B/antagonists & inhibitors , Cathepsin B/chemistry , Cathepsin L/antagonists & inhibitors , Cathepsin L/chemistry , Cryptosporidium parvum/metabolism , Cystatins/genetics , Cystatins/metabolism , Cysteine Proteases/chemistry , Cysteine Proteases/genetics , Cysteine Proteinase Inhibitors/genetics , Cysteine Proteinase Inhibitors/metabolism , Hot Temperature , Humans , Kinetics , Models, Molecular , Molecular Sequence Data , Papain/antagonists & inhibitors , Papain/chemistry , Protein Stability , Protein Structure, Secondary , Protozoan Proteins/chemistry , Protozoan Proteins/genetics , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Sequence Alignment
6.
J Int Med Res ; 40(6): 2083-9, 2012.
Article in English | MEDLINE | ID: mdl-23321164

ABSTRACT

OBJECTIVES: To investigate the expression and clinical significance of the protein tyrosine phosphatase, nonreceptor type 11 (PTPN11 or SHP2) gene, which encodes Src homology 2 domain-containing phosphatase (SHP-2) in gastric cancer. METHODS: SHP2 expression was detected by immunohistochemical staining and real-time quantitative reverse transcription-polymerase chain reaction in tissue samples of normal gastric mucosa and different grades of gastric cancer. Correlation between SHP2 expression and standard clinico pathological parameters was analysed. RESULTS: Immunohistochemical staining revealed significantly higher rates of SHP2 expression in gastric cancer tissues (72.5%) versus normal gastric mucosa (21.9%). SHP-2 mRNA levels were also significantly higher in gastric cancer tissues versus normal gastric mucosa. SHP2 expression correlated significantly with tumour differentiation, clinical classification and lymph node metastases, but was independent of sex and age. CONCLUSIONS: SHP-2 is upregulated in gastric cancer and may be related to the development of gastric cancer. SHP-2 may be a potential prognostic marker of, or a therapeutic target for, gastric cancer.


Subject(s)
Gastric Mucosa/metabolism , Protein Tyrosine Phosphatase, Non-Receptor Type 11/biosynthesis , Protein Tyrosine Phosphatase, Non-Receptor Type 11/metabolism , Stomach Neoplasms/metabolism , Biomarkers, Tumor , Female , Gene Expression Regulation, Neoplastic , Humans , Immunohistochemistry , Lymphatic Metastasis , Male , Middle Aged , Prognosis , Protein Tyrosine Phosphatase, Non-Receptor Type 11/genetics , RNA, Messenger/biosynthesis , Stomach Neoplasms/genetics , Survival Rate
7.
J Parasitol ; 89(3): 639-42, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12880278

ABSTRACT

Cryptosporidium parvum is 1 of the major causative organisms in waterborne diarrheal illness. Not only does C. parvum spread ubiquitously in our environment, it is also highly resistant to harsh environmental conditions and disinfectants. Therefore, a control measure for this protozoon is urgently required. This study investigated the effect of gamma-irradiation, in the range of 1,000-50,000 Gy, on the viability of C. parvum oocysts. Oocyst viability was determined by a combined indirect immunofluorescence and nucleic acid staining and animal infectivity study. The proportion of viable oocysts estimated by nucleic acid staining ranged from 94.2 to 89.4% in the 0- to 10,000-Gy groups, whereas it was reduced significantly to 58.6 or 45.7% in the 25,000- or 50,000-Gy group, respectively, at 24 hr postirradiation. In an animal infectivity study, oocysts irradiated with less than 10,000 Gy induced infections in mice wherein there were low numbers of oocysts per gram of feces amounting to 8-10.8% of the values in control mice, whereas with 50,000 Gy-irradiated oocysts, no oocysts were produced in the mice. This study suggests that at least 50,000 Gy of gamma-irradiation is necessary for the complete elimination of oocyst infectivity in mice.


Subject(s)
Cryptosporidium parvum/radiation effects , Gamma Rays , Animals , Cryptosporidium parvum/immunology , Cryptosporidium parvum/physiology , Dose-Response Relationship, Radiation , Female , Mice , Mice, Inbred C57BL , Specific Pathogen-Free Organisms
8.
Parasitol Res ; 88(5): 412-20, 2002 May.
Article in English | MEDLINE | ID: mdl-12049457

ABSTRACT

Two hybridoma clones, CMYL3 and CMYL30, were generated by immunizing Balb/c mice with excysted oocysts of Cryptosporidium muris. Both clones secreted monoclonal antibodies against an oocyst-wall antigen with apparent molecular mass of 250 kDa (called CM250) from C. muris and C. parvum. The epitope appeared to be periodate-sensitive, suggesting the involvement of a carbohydrate moiety. Immunofluorescence and confocal microscopy on purified oocysts and infected mouse tissues revealed staining confined to the oocyst wall of both Cryptosporidium species. Immunogold labeling further revealed the presence of the CM250 antigen in electron-dense vesicles and cytoplasm of developing macrogametocytes, and ultimately localized to the oocyst wall of mature oocysts. Both antibodies cross-reacted with C. serpentis oocysts but did not recognize the other enteropathogenic protozoans Giardia muris, Eimeria falciformis and E. nischulz. These antibodies may be valuable tools for the analysis of oocyst-wall formation in Cryptosporidium and characterization of the common antigen.


Subject(s)
Antibodies, Monoclonal/immunology , Antigens, Protozoan/immunology , Antigens, Surface/immunology , Cryptosporidium/immunology , Oocysts/immunology , Animals , Antigens, Protozoan/chemistry , Antigens, Surface/chemistry , Cryptosporidium/growth & development , Female , Fluorescent Antibody Technique, Indirect , Hybridomas , Immunohistochemistry , Mice , Mice, Inbred BALB C , Microscopy, Confocal
9.
Mol Biol Cell ; 12(9): 2835-45, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11553721

ABSTRACT

Calreticulin (CRT), a Ca(2+)-binding protein known to have many cellular functions, including regulation of Ca(2+) homoeostasis and chaperone activity, is essential for heart and brain development during embryogenesis in mice. Here, we report the functional characterization of Caenorhabditis elegans calreticulin (crt-1). A crt-1 null mutant does not result in embryonic lethality but shows temperature-dependent reproduction defects. In C. elegans CRT-1 is expressed in the intestine, pharynx, body-wall muscles, head neurons, coelomocytes, and in sperm. crt-1 males exhibit reduced mating efficiency and defects late in sperm development in addition to defects in oocyte development and/or somatic gonad function in hermaphrodites. Furthermore, crt-1 and itr-1 (inositol triphosphate receptor) together are required for normal behavioral rhythms. crt-1 transcript level is elevated under stress conditions, suggesting that CRT-1 may be important for stress-induced chaperoning function in C. elegans.


Subject(s)
Caenorhabditis elegans/physiology , Calcium-Binding Proteins/metabolism , Calcium/metabolism , Molecular Chaperones/metabolism , Ribonucleoproteins/metabolism , Animals , Blotting, Northern , Blotting, Western , Caenorhabditis elegans/genetics , Caenorhabditis elegans/metabolism , Caenorhabditis elegans Proteins/genetics , Caenorhabditis elegans Proteins/metabolism , Calcium Channels/metabolism , Calcium-Binding Proteins/genetics , Calreticulin , Fertility/genetics , Gene Deletion , Gene Expression Profiling , Homeostasis , Immunohistochemistry , In Situ Hybridization , Inositol 1,4,5-Trisphosphate Receptors , Intestinal Mucosa/metabolism , Male , Molecular Chaperones/genetics , Muscles/metabolism , Pharynx/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Cytoplasmic and Nuclear/metabolism , Ribonucleoproteins/genetics , Spermatozoa/metabolism , Temperature
10.
Korean J Parasitol ; 39(2): 193-6, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11441507

ABSTRACT

We report a case of gastric pseudoterranoviasis proven by gastrofiberscopy on Dec. 13, 1994. The 34-year-old male patient, residing in Chungju-shi, was admitted to Konkuk University Hospital complaining of prickling epigastric pain. The symptoms suddenly attacked him two days after eating raw marine fish at Chonan-shi. By the gastrofiberscopic examination, a long white-yellowish nematode was found from the fundus region of stomach. The worm was 34.50 x 0.84 mm in size, and was identified as a 3rd stage larva of Pseudoterranova decipiens judging from the position of the intestinal cecum. This is the 12th confirmed case of human pseudoterranoviasis in Korea.


Subject(s)
Anisakiasis/parasitology , Ascaridoidea/isolation & purification , Stomach Diseases/parasitology , Adult , Animals , Gastric Mucosa/parasitology , Gastroscopy , Humans , Korea , Larva , Male
11.
Korean J Parasitol ; 39(2): 201-3, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11441509

ABSTRACT

The present study was undertaken to know the infection status of Cryptosporidium parvum among the residents of Chorwon-gun, Kangwon-do in 1993. Total 461 fecal samples were collected from the inhabitants residing in Chorwon-gun during the period of August 12 to September 14, 1993. Fecal smears were prepared by formalin-ether sedimentation, and examined after modified acid fast staining. Of the 461 fecal samples, 9 (1.9%) were positive for C. parvum oocysts. The positive cases were limited to thirties (4) patients, forties (3), and sixties (2), and no oocyst was detected in other age groups. The oocyst positive rate for male was 1.4% and that of female was 2.6%.


Subject(s)
Cryptosporidiosis/epidemiology , Adolescent , Adult , Age Factors , Aged , Animals , Child , Cryptosporidiosis/parasitology , Cryptosporidium parvum/isolation & purification , Feces/parasitology , Female , Humans , Korea/epidemiology , Male , Middle Aged , Parasite Egg Count , Sex Factors
12.
Korean J Parasitol ; 39(1): 13-21, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11301586

ABSTRACT

Pneumocystis carinii causes serious pulmonary infection in immunosuppressed patients. This study was undertaken to observe the cytoskeletal proteins of P. carinii by immuno-electron microscopy. P. carinii infection was experimentally induced by immunosuppression of Sprague-Dawley rats for seven weeks, and their lungs were used for the observations of this study. The gold particles localized actin, tropomyosin, and tubulin. The actin was irregularly scattered in the cytoplasm of the trophic forms but was much more concentrated in the inner space of the cell wall of the cystic forms called the inner electron-lucent layer. No significant amount of tropomyosin was observed in either trophic forms or cystic forms. The tubulin was distributed along the peripheral cytoplasm and filopodia of both the trophic and cystic forms rather than in the inner side of the cytoplasm. Particularly, in the cystic forms, the amount of tubulin was increased and located mainly in the inner electron-lucent layer of the cell wall where the actin was concentrated as well. The results of this study showed that the cell wall of P. carinii cystic forms is a structure whose inner side is rich in actin and tubulin. The location of the actin and tubulin in P. carinii suggests that the main role of these proteins is an involvement in the protection of cystic forms from the outside environment by maintaining rigidity of the cystic forms.


Subject(s)
Cytoskeletal Proteins/analysis , Fungal Proteins/analysis , Pneumocystis/chemistry , Actins/analysis , Animals , Histocytochemistry , Microscopy, Immunoelectron , Pneumocystis/cytology , Rats , Rats, Wistar , Tropomyosin/analysis , Tubulin/analysis
13.
Korean J Parasitol ; 38(3): 159-66, 2000 Sep.
Article in English | MEDLINE | ID: mdl-11002651

ABSTRACT

The present study intended to verify activities of cysteine proteinase of Pneumocystis carinii from rats and to purify the enzyme. In order to exclude the contamination of host-derived enzymes, concentrates of P. carinii was primarily treated with a mixture of proteinase inhibitors before lysis of P. carinii. A 68-kDa cysteine proteinase was finally purified from the crude extract of P. carinii by 4 sequential chromatographic methods. The enzyme showed an optimal activity at pH 5.5 in 0.1 M sodium acetate, and its activity was specifically inhibited by L-trans-epoxy-succinylleucylamido (4-guanidino) butane (E-64) and iodoacetic acid, suggesting that the enzyme is a cysteine proteinase. The 68-kDa proteinase weakly digested macromolecules such as collagen, hemoglobin and fibronectin. The present study demonstrated the activity of cysteine proteinase at the 68-kDa band of P. carinii, and purified and characterized the molecule.


Subject(s)
Cysteine Endopeptidases/isolation & purification , Pneumocystis/enzymology , Animals , Collagen/metabolism , Cysteine Endopeptidases/pharmacology , Fibronectins/metabolism , Hemoglobins/metabolism , Macromolecular Substances , Molecular Weight , Rats , Rats, Wistar
14.
Korean J Parasitol ; 38(3): 151-8, 2000 Sep.
Article in English | MEDLINE | ID: mdl-11002650

ABSTRACT

Pneumocystis carinii is a major opportunistic pathogen which has been found in the lungs of a wide variety of mammalian host species, and the fact suggests the possibility of intraspecific variation. Until now, P. carinii from different mammalian species are differentiated as subspecies, and the rats are known to be infected by two subspecies. The present study investigated genetic heterogeneity of P. carinii isolates from two strains of rats in Korea and China by molecular karyotyping, RFLP and sequencing analysis. Karyotypes of P. carinii were grouped into three, two from two strains of rats in Korea and one from rats in China. However RFLP of PCR product of ribosomal and MSG gene of the P. carinii isolates showed same pattern. The sequence homology rates of alpha-tubulin DNA of the P. carinii isolates were 96% in Seoul Wistar rats, 93% in Seoul Sprague-Dawley rats, and 85% in Chinese Sprague-Dawley rats. The present finding confirmed that P. carinii from rats in Korea are grouped into two karyotype strains which are different from that of P. carinii from rats in China. The Chinese isolate shows a little different sequences of alpha-tubulin DNA.


Subject(s)
Genetic Heterogeneity , Pneumocystis/genetics , Rats, Sprague-Dawley/microbiology , Rats, Wistar/microbiology , Animals , Base Sequence , China , DNA, Fungal/genetics , Karyotyping , Korea , Molecular Sequence Data , Pneumocystis/isolation & purification , Polymorphism, Restriction Fragment Length , Rats , Sequence Analysis, DNA , Tubulin/genetics
15.
Korean J Parasitol ; 38(2): 59-64, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10905066

ABSTRACT

To determine a suitable condition for in vitro infection model of Cryptosporidium parvum, four different cell lines, AGS, MDCK, HCT-8 and Caco-2, were used as host cell lines which were cultured at various concentrations of added supplements. These supplement include fetal bovine serum (FBS), sodium choleate, ascorbic acid, folic acid, calcium pantothenate, para-aminobenzoic acid and pyruvate and their effects on the cell lines which were infected with C. parvum were evaluated. The results of this study showed that the AGS cell line was most susceptible to C. parvum whereas the Caco-2 cells appeared to be least susceptible to C. parvum. In regards to the serum condition, 10% FBS was suitable for the growth of AGS and HCT-8 cells, and 1% FBS was good for the growth of the MDCK cells when they were inoculated with C. parvum. Vitamins had a positive effect on the AGS cells, and pyruvate also showed positive effects on all of the cell lines except for Caco-2. Modified medium for each cell line was prepared by adding appropriate amounts of each supplement which resulted in the highest parasite infection number. Modified media increased the number of parasites infected on AGS cells to 2.3-fold higher when compared to the control media. In this study, we found that the AGS cell line was a suitable host model for evaluating C. parvum in vitro study and the media contents for the optimal infection conditions were suggested.


Subject(s)
Cryptosporidium parvum/growth & development , Animals , Culture Media , Humans , Mice , Tumor Cells, Cultured
16.
Korean J Parasitol ; 38(4): 257-61, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11138319

ABSTRACT

This study was focused on the effects of microfilament inhibitor, Cytochalasin D (CD) on the invasiveness of sporozoites of Cryptosporidium spp. into the host cells. MDCK and AGS cell lines were used as host cells for C. parvum and C. muris, respectively. When MDCK cells were pretreated with CD for 1 hr before inoculation of the sporozoites, C. parvum infection was significantly inhibited when compared to the control cells. These inhibitory effects of CD on the rate of infection were dose-dependent. In addition, C. muris infection was hampered when AGS cell lines were pretreated with CD. However, the capability of invasiveness of the sporozoites into the host cells was not greatly influenced by the pretreatment of sporozoites with CD before infection. These results suggest that microfilaments of host cells, rather than parasites, play an important role for the invasion of Cryptosporidium spp.


Subject(s)
Actin Cytoskeleton/drug effects , Cryptosporidiosis/drug therapy , Cytochalasin D/therapeutic use , Actin Cytoskeleton/physiology , Animals , Cells, Cultured , Cryptosporidiosis/etiology , Cryptosporidium/pathogenicity , Cytochalasin D/pharmacology , Dogs , Dose-Response Relationship, Drug , Humans
17.
Korean J Parasitol ; 37(2): 109-15, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10388269

ABSTRACT

The present study observed the variation of antigenicity of Pneumocystis carinii and serum IgG antibody reaction to the antigens from different localities in Korea. Antigens of rat P. carinii and sera of inhabitants were collected at Chunchon. Chungju, Kwangju, and Seoul during 1995-1996. Enzyme-linked Immunosorbent Assay and immunoblot were used for immune reaction. Absorbance of 1,294 human sera ranged between 0.01 and 0.93. Sera from Chunchon showed higher absorbances than those from other areas. Immunoblotting revealed IgG antibody reactions to 116, 100, and 45-55 kDa antigenic bands of rat P. carinii, but the frequencies of positive reaction to individual bands were variable by localities. Total 62.6% of the sera showed the reaction to 116 kDa band while 37.7% reacted to 100 kDa band and 32.0% to 45-55 kDa bands. For the reaction to 116 kDa, the reaction rate was 60.0% to 82.6% by localities. It is found that the reaction rates of the human sera to rat P. carinii antigen are variable according to the localities. Also, the high molecular antigen of 116 kDa of rat P. carinii is the most frequent antigenic band reacting to human sera.


Subject(s)
Antibodies, Fungal/blood , Antigens, Fungal/immunology , Immunoglobulin E/blood , Pneumocystis/immunology , Animals , Enzyme-Linked Immunosorbent Assay , Female , Humans , Korea/epidemiology , Male , Molecular Weight , Pneumocystis Infections/epidemiology , Pneumocystis Infections/immunology , Rats , Rats, Sprague-Dawley , Rats, Wistar , Seroepidemiologic Studies
18.
Am J Sports Med ; 27(3): 339-44, 1999.
Article in English | MEDLINE | ID: mdl-10352770

ABSTRACT

The specific aim of this study was to quantify glenohumeral translations in cadaveric shoulders after repair of the superior and middle regions of a surgically created Bankart lesion and after repair of the superior, middle, and inferior regions of the same lesion. Anterior-posterior, superior-inferior, and medial-lateral translations in nine cadaveric specimens were tested with shoulders in 0 degree, 45 degrees, and 90 degrees of humeral abduction and varying degrees of humeral rotation. There was statistically significantly less anterior and inferior translation after three-site labral repair compared with after two-site labral repair, and this effect was greatest at 90 degrees of glenohumeral abduction. The decreased translations demonstrated with three-site repair emphasized the importance of careful repair of the labrum to the inferior glenoid rim during a Bankart reconstruction and suggested that failure to do so may be a contributing factor to recurrent instability after anterior shoulder reconstruction.


Subject(s)
Ligaments, Articular/surgery , Shoulder Dislocation/surgery , Aged , Aged, 80 and over , Biomechanical Phenomena , Cadaver , Female , Humans , Humerus/physiology , Male , Range of Motion, Articular
19.
Korean J Parasitol ; 36(4): 227-34, 1998 Dec.
Article in English | MEDLINE | ID: mdl-9868887

ABSTRACT

Actin and tropomyosin of Cryptosporidium muris were localized by immunogold labeling. Two kinds of antibodies for actin labeling were used. The polyclonal antibody to skeletal muscle (chicken back muscle) actin was labeled on the pellicle and cytoplasmic vacuoles of parasites. The feeder organelle has showed a small amount of polyclonal actin antibody labeling as well. Whereas the monoclonal antibody to smooth muscle (chicken gizzard muscle) actin was chiefly labeled on the filamentous cytoplasm of parasites. The apical portion of host gastric epithelial cell cytoplasm was also labeled by smooth muscle actin together. The polyclonal antibody to tropomyosin was much more labeled at C. muris than host cells, so it could be easily identified even with low magnification (x2,000). The tropomyosin was observed along the pellicle, cytoplasmic vacuoles, and around the nucleus also. The skeletal muscle type actin seems to play a role in various cellular functions with tropomyosin in C. muris; on the other hand, the smooth muscle type actin was located mainly on the filamentous cytoplasm and supported the parasites' firm attachment to host cells. Tropomyosin on the pellicle was thought to be able to stimulate the host as a major antigen through continuous shedding out by the escape of sporozoites or merozoites from their mother cells.


Subject(s)
Actins/analysis , Cryptosporidium/chemistry , Tropomyosin/analysis , Actins/physiology , Animals , Cryptosporidium/cytology , Cryptosporidium/physiology , Cytoplasm/chemistry , Immunohistochemistry , Mice , Mice, Inbred C57BL , Tropomyosin/physiology
20.
Korean J Parasitol ; 36(4): 217-25, 1998 Dec.
Article in English | MEDLINE | ID: mdl-9868886

ABSTRACT

A scanning electron microscopic study was performed to observe surface ultrastructures of excysted metacercariae and adults of Metagonimus miyatai. Metacercariae were collected from the scale of the pale chub (Zacco platypus), and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae, the oral sucker was devoid of tegumental spines and had type I and type II sensory papillae. Anteriorly to the ventral sucker, spines were dense and digitated into 5-7 points, whereas near the posterior end of the body spines were sparse and digitated into 2-3 points. In one-week adults, 7 type II sensory papillae were arranged around the lip of the oral sucker, and at inner side of the lip one pair of small and two pairs of large type 1 sensory papillae were seen on each side. The distribution of tegumental spines was similar to that of metacercariae, but they were more differentiated with 9-11 pointed tips. In two- to four-week old adults, the surface ultrastructure was nearly the same as in one-week old adults, however, sperms were frequently seen entering into the Laurer's canal. Conclusively, the surface ultrastructure of M. miyatai was generally similar to that of M. yokogawai, however, differentiation of tegumental spines and distribution of sensory papillae around the oral sucker were different between the two species, which may be of taxonomic significance.


Subject(s)
Heterophyidae/ultrastructure , Animals , Fishes , Heterophyidae/growth & development , Microscopy, Electron, Scanning , Rats , Rats, Sprague-Dawley
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