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Virus Genes ; 42(3): 394-401, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21369826

ABSTRACT

It is well known that Norovirus (NoV) and Sapovirus (SaV) identified in humans and pigs have heterogeneous genome sequences. In this study, a total of three strains of NoV and 37 strains of SaV were detected in 567 porcine fecal samples by RT-PCR, corresponding detection rates of 0.5 and 6.5%, respectively. Phylogenetic analyses were conducted using amino acid sequences of the partial RNA-dependent RNA polymerase (RdRp) and complete capsid proteins of both viruses to determine their genogroups. Analysis with the RdRp sequences indicated that all three NoV strains HW41, DG32, and DO35 detected in this study were classified into genogroup II (GII). A further analysis with the complete capsid sequence demonstrated that the DO35 strain belonged to subgenotype b in GII-21 (GII-21b) along with the SW918 strain. A total of 26 strains out of 27 strains that were selected from the 37 porcine SaVs were classified into genogroup III when they were analyzed with the RdRp sequences. The remaining strain (DO19) was not clustered with any of the previously classified SaV strains, thereby suggesting the advent of a new genogroup virus. Additional analyses with the amino acid sequence of the capsid and the nucleotide sequence of the RdRp and capsid junction region supported the notion that the DO19 strain belonged to a novel genogroup of SaV. To the best of our knowledge, this is the first report to describe a novel porcine SaV belonging to an unknown genogroup in Korea.


Subject(s)
Caliciviridae Infections/veterinary , Genetic Variation , Norovirus/genetics , Norovirus/isolation & purification , Sapovirus/genetics , Sapovirus/isolation & purification , Swine Diseases/virology , Animals , Base Sequence , Caliciviridae Infections/virology , Capsid Proteins/genetics , Feces/virology , Norovirus/classification , Norovirus/enzymology , Phylogeny , RNA-Dependent RNA Polymerase/genetics , Republic of Korea , Reverse Transcriptase Polymerase Chain Reaction , Sapovirus/classification , Sapovirus/enzymology , Swine
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