Time characteristics of aero-optical imaging degeneration of the ellipsoidal dome under variable operating conditions.

As a missile flies at high speed, the index and surface shape of the conformal dome will vary under the influence of the aero-optical effect, which will degenerate the performance of the seeker's imaging detection system. However, many previous studies on aero-optical imaging deterioration of optical domes were usually carried out under fixed operating conditions, which are not in line with the real flight scene of the missile. In addition, the aero-optical imaging degeneration of the dome is diverse as the flight time increases. Therefore, it is of great significance to study the time characteristics of aero-optical imaging degradation of optical domes under variable work conditions. In this study, taking an air-to-air missile as an example, the Zernike polynomials, wavefront aberration, Strehl ratio, and image simulation are applied to evaluate the aero-optical imaging deterioration of an ellipsoidal dome in the flight time range of 0-10 s under variable working conditions. The simulation results show that, as the flight time increases, (1) the dynamic range of tilt, defocus, astigmatism, coma, and wavefront aberration increase; and (2) the Strehl ratio and the peak signal-to-noise ratio (PSNR) of the simulated images decrease. Therefore, the influence of flight time on the aero-optical image degradation of the ellipsoidal dome gradually becomes more serious.

The brain entropy dynamics in resting state.

As a novel measure for irregularity and complexity of the spontaneous fluctuations of brain activities, brain entropy (BEN) has attracted much attention in resting-state functional magnetic resonance imaging (rs-fMRI) studies during the last decade. Previous studies have shown its associations with cognitive and mental functions. While most previous research assumes BEN is approximately stationary during scan sessions, the brain, even at its resting state, is a highly dynamic system. Such dynamics could be characterized by a series of reoccurring whole-brain patterns related to cognitive and mental processes. The present study aims to explore the time-varying feature of BEN and its potential links with general cognitive ability. We adopted a sliding window approach to derive the dynamical brain entropy (dBEN) of the whole-brain functional networks from the HCP (Human Connectome Project) rs-fMRI dataset that includes 812 young healthy adults. The dBEN was further clustered into 4 reoccurring BEN states by the k-means clustering method. The fraction window (FW) and mean dwell time (MDT) of one BEN state, characterized by the extremely low overall BEN, were found to be negatively correlated with general cognitive abilities (i.e., cognitive flexibility, inhibitory control, and processing speed). Another BEN state, characterized by intermediate overall BEN and low within-state BEN located in DMN, ECN, and part of SAN, its FW, and MDT were positively correlated with the above cognitive abilities. The results of our study advance our understanding of the underlying mechanism of BEN dynamics and provide a potential framework for future investigations in clinical populations.

Ocular mucosal homeostasis of teleost fish provides insight into the coevolution between microbiome and mucosal immunity.

BACKGROUND: The visual organ plays a crucial role in sensing environmental information. However, its mucosal surfaces are constantly exposed to selective pressures from aquatic or airborne pathogens and microbial communities. Although few studies have characterized the conjunctival-associated lymphoid tissue (CALT) in the ocular mucosa (OM) of birds and mammals, little is known regarding the evolutionary origins and functions of immune defense and microbiota homeostasis of the OM in the early vertebrates. RESULTS: Our study characterized the structure of the OM microbial ecosystem in rainbow trout (Oncorhynchus mykiss) and confirmed for the first time the presence of a diffuse mucosal-associated lymphoid tissue (MALT) in fish OM. Moreover, the microbial communities residing on the ocular mucosal surface contribute to shaping its immune environment. Interestingly, following IHNV infection, we observed robust immune responses, significant tissue damage, and microbial dysbiosis in the trout OM, particularly in the fornix conjunctiva (FC), which is characterized by the increase of pathobionts and a reduction of beneficial taxa in the relative abundance in OM. Critically, we identified a significant correlation between viral-induced immune responses and microbiome homeostasis in the OM, underscoring its key role in mucosal immunity and microbiota homeostasis. CONCLUSIONS: Our findings suggest that immune defense and microbiota homeostasis in OM occurred concurrently in early vertebrate species, shedding light on the coevolution between microbiota and mucosal immunity. Video Abstract.

Mucosal immune responses of gut IgM in common carp (Cyprinus carpio) following infection with spring viremia of carp virus (SVCV).

Immunoglobulin M (IgM) specifically recognizes various antigens and can activate complement, mediate cytotoxicity, opsonize and agglutinate pathogens to induce phagocytosis, all of which play an important role in immunity. However, the IgM response of common carp (Cyprinus carpio) in the intestinal mucosa after viral infection has not been thoroughly. Therefore, we successfully produced an anti-carp IgM monoclonal antibody and developed a model of viral infection to study the kinetics of immune responses after viral infection. Our results showed that the expression of IL1-ß and Igs were dramatically increased, implying that common carp exhibited a significant innate and adaptive immune response to viral infection. Furthermore, we found that the IgM responses varied between the two infection strategies. At 14 days post-infection (DPI), a significant population of IgM+ B cells were observed in the gut, accompanied by a sharp rise in IgM levels. The immune response to secondary infection started at 7 DPI, suggesting that the IgM response is faster in the gut after re-infection. Importantly, we also explored the variability of different gut compartments to viral infection, and result revealed a stronger immune response in the hindgut than in the foregut and midgut. Overall, our findings indicate that IgM plays an important role in the intestinal immune response following primary and secondary viral infection, in which the hindgut plays a major immune function.

Response of immunoglobulin M in gut mucosal immunity of common carp (Cyprinus carpio) infected with Aeromonas hydrophila.

Immunoglobulin (Ig) M is an important immune effector that protects organisms from a wide variety of pathogens. However, little is known about the immune response of gut mucosal IgM during bacterial invasion. Here, we generated polyclonal antibodies against common carp IgM and developed a model of carp infection with Aeromonas hydrophila via intraperitoneal injection. Our findings indicated that both innate and adaptive immune responses were effectively elicited after A. hydrophila infection. Upon bacterial infection, IgM+ B cells were strongly induced in the gut and head kidney, and bacteria-specific IgM responses were detected in high levels both in the gut mucus and serum. Moreover, our results suggested that IgM responses may vary in different infection strategies. Overall, our findings revealed that the infected common carp exhibited high resistance to this representative enteropathogenic bacterium upon reinfection, suggesting that IgM plays a key role in the defense mechanisms of the gut against bacterial invasion. Significantly, the second injection of A. hydrophila induces strong local mucosal immunity in the gut, which is essential for protection against intestinal pathogens, providing reasonable insights for vaccine preparation.

Analog optical deconvolution computing for wavefront coding based on nanoantennas metasurfaces.

Analog optical computing based on metasurfaces has attracted much attention for achieving high-speed calculating without the electronic processing unit. Wavefront coding imaging systems involve the joint design of an encoded image-capturing module and decoding postprocessing algorithms to obtain a required final image. The decoding postprocessing algorithms, as a typical deconvolution computation, require an additional electronic processing unit to yield a high-quality decoded image. We demonstrate an analog optical deconvolution computing kernel based on nanoantennas metasurfaces for the postprocessing calculation of wavefront coding systems. Numerical simulations are presented to prove that the encoded point spread function can be refocused through a designed optical computing metasurface. The proposed approach opens an opportunity for real-time recovering images in wavefront coding optical systems.

A potential alternative to traditional antibiotics in aquaculture: Yeast glycoprotein exhibits antimicrobial effect in vivo and in vitro on Aeromonas caviae isolated from Carassius auratus gibelio.

In aquaculture, antibiotics are commonly used to provide protection against pathogens; however, this practice has become controversial due to increased occurrences of microbial resistance, and alternatives are needed. This study aimed to investigate the antimicrobial activity of yeast glycoprotein (YG) against Aeromonas caviae. Pathogens were isolated from liver of diseased Carassius auratus gibelio. Based on morphological and biochemical analysis, together with 16S rRNA gene sequencing, the isolated strains were identified as A. caviae and concluded as clones of a single strain and named L2. Further pathogenicity analysis revealed that A. caviae possessed ß-haemolysis, and its median lethal dose for C. gibelio was 1.33 × 106  CFU/ml. Hepatic adenylate kinase and pyruvate kinase activities of C. gibelio were inhibited post-A. caviae infection. Antimicrobial drug test suggested that A. caviae was a multidrug-resistant organism but could be inhibited by YG in vitro. Minimum inhibitory and bactericidal concentration of YG was 83.3 mg/ml and 166.7 mg/ml, respectively. Microbiota sequencing results showed that YG supplement could obviously decrease the relative abundance of Aeromonas and increase the microbial diversity. Our study revealed that A. caviae from C. gibelio was a multidrug-resistant bacteria strain, and could be significantly inhibited by YG in vivo and in vitro, thus providing important insights into ecological control and pathogenesis of A. caviae in aquaculture.

The Lovász Hinge: A Novel Convex Surrogate for Submodular Losses.

Learning with non-modular losses is an important problem when sets of predictions are made simultaneously. The main tools for constructing convex surrogate loss functions for set prediction are margin rescaling and slack rescaling. In this work, we show that these strategies lead to tight convex surrogates iff the underlying loss function is increasing in the number of incorrect predictions. However, gradient or cutting-plane computation for these functions is NP-hard for non-supermodular loss functions. We propose instead a novel surrogate loss function for submodular losses, the Lovász hinge, which leads to O(p logp) complexity with O(p) oracle accesses to the loss function to compute a gradient or cutting-plane. We prove that the Lovász hinge is convex and yields an extension. As a result, we have developed the first tractable convex surrogates in the literature for submodular losses. We demonstrate the utility of this novel convex surrogate through several set prediction tasks, including on the PASCAL VOC and Microsoft COCO datasets.

Influence of altitude on aero-optic imaging quality degradation of the hemispherical optical dome.

We investigated the influence of altitude on aero-optic imaging quality degradation of the hemispherical optical dome. Boundary conditions for the aerodynamic heating effect of the optical dome were calculated by solving the Reynolds-averaged Navier-Stokes equations provided by FLUENT. The finite element model and the thermal-structure simulation results of the optical dome were obtained using ANSYS. The 3D nonuniform refractive index field of the optical dome was obtained according to the thermal-optical effect. The optical tracking method based on the fourth-order Runge-Kutta algorithm was adopted to simulate the optical transmission through the optical dome. The Strehl ratio (SR), encircled energy, distorted target images, and peak signal-to-noise ratio were presented for imaging quality evaluation. The variation rules of these imaging quality evaluation parameters were obtained in the altitude range of 0-45 km. The results showed that, in the same flight conditions, with the increase of altitude, peak signal-to-noise ratio (PSNR) of the distorted image, and SR result were increased, and radiuses of dispersion spots, including 80% energy, were decreased; therefore, the influence of aero-optics effect on imaging quality degradation was gradually weakened.

Basic geometry and aberration characteristics of conicoidal conformal domes.

This paper investigated the geometry and aberration characteristics of conicoidal conformal domes. First, on the basis of previous research, we got the expression that was suitable for describing the external surface of the conicoidal conformal dome. Based on the theory of differential geometry, this paper first proved that the Dupin index line of a quadric surface was an ellipsoid and the radius of curvature had extreme values in the meridian plane and sagittal plane. Then the uniform formulas of curvature which were suitable for ellipsoid, paraboloid, and hyperboloid were deduced in the meridian plane and sagittal plane, respectively. Meanwhile, the angle between the axis of imaging systems and the surface normal was calculated. With the help of computers, the plots of curvature differences and the angle in the case of different edge slopes, fineness ratios, and the locations of the rotational center were obtained. Finally, we analyzed the Zernike polynomial coefficients of Z4, Z5, and Z8, which represent defocus, astigmatism and coma, respectively for the model established in CODE V. The research indicates that the dynamic ranges of defocus, astigmatism, and coma increase with the growing of edge slopes and fineness ratios, but have little change with the variation of the rotational center positions. Moreover, the curves of Z5 and Z8 have turning points, and the curves of curvature differences and angle difference are only similar to the curves of Z5 and Z8 when the look angle changes after the turning point. For the look angle changing from zero to the turning point, the curves of Z5 and Z8 change rapidly. This is mainly caused by the significant variations of the symmetry of the conformal dome participating in imaging. Therefore, the aberrations with small scanning angles should be given more attention when designing the conformal systems.

One-Step Self-Assembling Method to Prepare Dual-Functional Transferrin Nanoparticles for Antitumor Drug Delivery.

Protein-based nanoparticles hold great promise in both preclinical and clinical practices due to their high biocompatibility and biodegradability. However, the complicated preparations often denature proteins, which subsequently diminish their bioactivity. To overcome these drawbacks, we developed a one-step self-assembling method for preparing protein-based nanoparticles. Transferrin (Tf), a targeting protein, was mixed with 2-mercaptoethanol to break disulfide bonds. Using this method, Tf-PTX-NPs (paclitaxel-loaded Tf nanoparticles) could be readily obtained. Tf-PTX-NPs were round and their diameter could be controlled in the range of 5-200 nm. The bioactivity of Tf to its receptor after forming nanoparticles was also confirmed in vitro. Tf-PTX-NPs also could inhibit the tumor growth to some extent in a mice tumor xenograft model. Therefore, using this self-assembling method, we fabricated this antitumor Tf-based nanoparticle, in which Tf acted as both the targeting moiety and drug carrier.