Research on a Cross-Domain Few-Shot Adaptive Classification Algorithm Based on Knowledge Distillation Technology.

With the development of deep learning and sensors and sensor collection methods, computer vision inspection technology has developed rapidly. The deep-learning-based classification algorithm requires the acquisition of a model with superior generalization capabilities through the utilization of a substantial quantity of training samples. However, due to issues such as privacy, annotation costs, and sensor-captured images, how to make full use of limited samples has become a major challenge for practical training and deployment. Furthermore, when simulating models and transferring them to actual image scenarios, discrepancies often arise between the common training sets and the target domain (domain offset). Currently, meta-learning offers a promising solution for few-shot learning problems. However, the quantity of supporting set data on the target domain remains limited, leading to limited cross-domain learning effectiveness. To address this challenge, we have developed a self-distillation and mixing (SDM) method utilizing a Teacher-Student framework. This method effectively transfers knowledge from the source domain to the target domain by applying self-distillation techniques and mixed data augmentation, learning better image representations from relatively abundant datasets, and achieving fine-tuning in the target domain. In comparison with nine classical models, the experimental results demonstrate that the SDM method excels in terms of training time and accuracy. Furthermore, SDM effectively transfers knowledge from the source domain to the target domain, even with a limited number of target domain samples.

Genome-wide identification and characterization of lipoxygenase genes related to the English grain aphid infestation response in wheat.

MAIN CONCLUSION: 44 wheat LOX genes were identified by silico genome-wide search method. TaLOX5, 7, 10, 24, 29, 33 were specifically expressed post aphid infestation, indicating their participation in wheat-aphid interaction. In plants, LOX genes play important roles in various biological progresses including seed germination, tuber development, plant vegetative growth and most crucially in plant signal transduction, stress response and plant defense against plant diseases and insects. Although LOX genes have been characterized in many species, the importance of the LOX family in wheat has still not been well understood, hampering further improvement of wheat under stress conditions. Here, we identified 44 LOX genes (TaLOXs) in the whole wheat genome and classified into three subfamilies (9-LOXs, Type I 13-LOXs and Type II 13-LOXs) according to phylogenetic relationships. The TaLOXs belonging to the same subgroup shared similar gene structures and motif organizations. Synteny analysis demonstrated that segmental duplication events mainly contributed to the expansion of the LOX gene family in wheat. The results of protein-protein interaction network (PPI) and miRNA-TaLOXs predictions revealed that three TaLOXs (TaLOX20, 22 and 37) interacted mostly with proteins related to methyl jasmonate (MeJA) signaling pathway. The expression patterns of TaLOXs in different tissues (root, stem, leaf, spike and grain) under diverse abiotic stresses (heat, cold, drought, drought and heat combined treatment, and salt) as well as under diverse biotic stresses (powdery mildew pathogen, Fusarium graminearum and stripe rust pathogen) were systematically analyzed using RNA-seq data. We obtained aphid-responsive candidate genes by RNA-seq data of wheat after the English grain aphid infestation. Aphid-responsive candidate genes, including TaLOX5, 7, 10, 24, 29 and 33, were up-regulated in the wheat aphid-resistant genotype (Lunxuan144), while they were little expressed in the susceptible genotype (Jimai22) during late response (48 h and 72 h) to the English grain aphid infestation. Meanwhile, qRT-PCR analysis was used to validate these aphid-responsive candidate genes. The genetic divergence and diversity of all the TaLOXs in bread wheat and its relative species were investigated by available resequencing data. Finally, the 3D structure of the TaLOX proteins was predicted based on the homology modeling method. This study not only systematically investigated the characteristics and evolutionary relationships of TaLOXs, but also provided potential candidate genes in response to the English grain aphid infestation and laid the foundation to further study the regulatory roles in the English grain aphid infestation of LOX family in wheat and beyond.

Construction of a Modified Clip Cage and Its Effects on the Life-History Parameters of Sitobion avenae (Fabricius) and Defense Responses of Triticum aestivum.

Clip cages are commonly used to confine aphids or other small insects to a single leaf when conducting plant-small insect interaction studies; however, clip cages are usually heavy or do not efficiently transmit light, which has an impact on leaf physiology, limiting their application. Here, simple, lightweight, and transparent modified clip cages were constructed using punched clear plastic cups, cut transparent polyvinyl chloride sheets, nylon organdy mesh, and bent duck-bill clips. These cages can be clipped directly onto dicot leaves or attached to monocot leaves with bamboo skewers and elastic bands. The weight, production time, and aphid escape rates of the modified clip cages were 3.895 ± 0.004 g, less than 3 min, and 2.154 ± 0.323%, respectively. The effects of the modified clip cage on the growth, development, and reproduction of the English grain aphid (Sitobion avenae Fabricius) in comparison with the whole cage were studied. The biochemical responses of wheat (Triticum aestivum) to the cages were also investigated. No significant differences were observed in the life table parameters, nymph mortality, and adult fecundity in S. avenae confined to clip cages and whole cages, but the clip cages were more time efficient than whole cages when conducting life table studies. Moreover, the hydrogen peroxide accumulation, callose deposition, and cell necrosis in wheat leaves covered by empty clip cages and empty whole cages were similar, and significantly lower than treatments where the aphids were inside the clip cage. The results demonstrate that the modified clip cages had negligible effects on the plant and aphid physiology, suggesting that they are effective for studying plant-small insect interactions.

Co4N-WNx composite for efficient piezocatalytic hydrogen evolution.

A dual-phase transition metal nitride (TMN) based Co4N-WNx system has been fabricated using nitridation of CoWO4. The interface between centrosymmetric Co4N and non-centrosymmetric WNx promotes charge carrier separation. This system also shows piezoelectric behavior. The piezoelectric property has been proved using piezoelectric force microscopy (PFM) measurements. In addition, modulating the non-centrosymmetric structure of Co4N-WNx allows a hydrogen production rate of about 262.7 µmol g-1 h-1 in pure water. We also show that the piezocatalytic hydrogen evolution efficiency is satisfactory. Co4N-WNx can also help achieve simultaneous piezocatalytic hydrogen production and RhB degradation. This work provides a novel strategy for designing efficient piezocatalytic materials.

RODFormer: High-Precision Design for Rotating Object Detection with Transformers.

Aiming at the problem of Transformers lack of local spatial receptive field and discontinuous boundary loss in rotating object detection, in this paper, we propose a Transformer-based high-precision rotating object detection model (RODFormer). Firstly, RODFormer uses a structured transformer architecture to collect feature information of different resolutions to improve the collection range of feature information. Secondly, a new feed-forward network (spatial-FFN) is constructed. Spatial-FFN fuses the local spatial features of 3 × 3 depthwise separable convolutions with the global channel features of multilayer perceptron (MLP) to solve the deficiencies of FFN in local spatial modeling. Finally, based on the space-FFN architecture, a detection head is built using the CIOU-smooth L1 loss function and only returns to the horizontal frame when the rotating frame is close to the horizontal, so as to alleviate the loss discontinuity of the rotating frame. Ablation experiments of RODFormer on the DOTA dataset show that the Transformer-structured module, the spatial-FFN module and the CIOU-smooth L1 loss function module are all effective in improving the detection accuracy of RODFormer. Compared with 12 rotating object detection models on the DOTA dataset, RODFormer has the highest average detection accuracy (up to 75.60%), that is, RODFormer is more competitive in rotating object detection accuracy.

Flow field characterization between vertical plate electrodes in a bench-scale cell of electrochemical water softening.

To analyze the effect of flow characteristics on electrochemical water softening, characteristics of flow fields in the vicinity of vertical plate electrodes in a bench-scale electrolysis cell for electrochemical water softening were visualized using particle image velocimetry technology, and the hardness drop values under different process conditions were measured. Properly increasing the current density or reducing the electrode spacing can increase the average flow velocity in the electrode gap. Excessive current density will cause bubble accumulation, form a bubble vortex, interfere with the orderly flow of surrounding liquid and reduce mass transfer efficiency. When the electrode spacing is 120 mm, the highest water softening efficiency measured at the current density of 60 A/m2 is 16.56%. When the current density is 50 A/m2, the highest average speed measured at the electrode spacing of 60 mm is 0.00169 m/s, but the highest water softening efficiency measured at the electrode spacing of 90 mm is 23.3%.The circulation efficiency in the electrode gap of a semi-closed structure is lower than that of a free convection structure. The behavior of bubbles is the key to flow and mass transfer. It is important to consider its influence on bubble behavior when optimizing electrochemical parameters.

Research on performance optimization and mechanism of electrochemical water softening applied by pulse power supply.

In order to promote the application of electrochemical water softening technology in industrial circulating cooling water systems, electric field type, cathode structure and solution residence time are selected for optimization analysis of an electrochemical water softening device. The experimental results show that the water softening performance per unit area of mesh cathode is better than that of a plate cathode. In addition, the softening amount per unit area of the mesh cathode can be further increased when the high-frequency (HF) power supply is applied. When the HF power supply is applied, the softening amount per unit area is 158.58 g/m2·h-1 more than when the direct current power supply is applied. In order to explore the growth mechanism of calcium carbonate, micro-analysis technology and high-speed bubble photography technology are used. The results show that the bubbles escape along the longitudinal direction of the electrode plate, and the main growth direction of calcium carbonate growth is consistent with the escape direction of the bubble; that is, the bubbles grow along the longitudinal direction of the electrode plate. The special structure of the diamond-shaped mesh cathode facilitates the growth of calcium carbonate crystals.

Optimal Heat Exchanger Area Distribution and Low-Temperature Heat Sink Temperature for Power Optimization of an Endoreversible Space Carnot Cycle.

Using finite-time thermodynamics, a model of an endoreversible Carnot cycle for a space power plant is established in this paper. The expressions of the cycle power output and thermal efficiency are derived. Using numerical calculations and taking the cycle power output as the optimization objective, the surface area distributions of three heat exchangers are optimized, and the maximum power output is obtained when the total heat transfer area of the three heat exchangers of the whole plant is fixed. Furthermore, the double-maximum power output is obtained by optimizing the temperature of a low-temperature heat sink. Finally, the influences of fixed plant parameters on the maximum power output performance are analyzed. The results show that there is an optimal temperature of the low-temperature heat sink and a couple of optimal area distributions that allow one to obtain the double-maximum power output. The results obtained have some guidelines for the design and optimization of actual space power plants.

Comparative analysis the performance of electrochemical water softening between high frequency electric fields and direct current electric fields based on orthogonal experimental methods.

Electrochemical water softening has been widely used in industrial circulating cooling water systems; however, their low deposition efficiency is the main drawback that limits usage in medium to large enterprises. In this work, the effect of different parameters on the hardness removal efficiency and energy consumption of the electrochemical water softening system is experimentally studied, and the performance of water softening applied by high frequency electric fields and direct current electric fields are comparative analyzed. The impact factors of the electrochemical water softening system are as follows: initial feed concentration of solute, magnitude of voltage, inter-electrode distance, area of cathode and frequency of power supply. To improve the analysis efficiency, the L25 (55) orthogonal table is used to investigate the five different factors at five levels. The experimental results are shown that the initial feed concentration of solute is the most significant factor affecting the hardness removal efficiency. The optimal combination for water softening in the group applied by high frequency electric field and direct current electric field are A3B2C1D4E3 and A2B5C3D1 respectively. The energy utilization of the device applied by high frequency electric field is 3.2 times that applied by direct current electric field. The practice shows that direct current electric fields have a better softening effect, and are is more suitable for scaling ion removal. Particle image velocimetry (PIV) was used to observe the flow field induced by the electrolysis and found that the vertical and horizontal velocities of the flow field at low voltage are conducive to the migration of scaled ions to the cathode, and then the electrolytic reaction and deposition reaction synergy effect is the optimal.

Correction to: Transplantation of human induced pluripotent stem cell-derived cardiomyocytes improves myocardial function and reverses ventricular remodeling in infarcted rat hearts.

The original article omits an affiliation for authors, He Zhang and Dongjin Wang. The omitted affiliation can be viewed in this Correction article.

Transplantation of human induced pluripotent stem cell-derived cardiomyocytes improves myocardial function and reverses ventricular remodeling in infarcted rat hearts.

BACKGROUND: Human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have shed great light on cardiac regenerative medicine and specifically myocardial repair in heart failure patients. However, the treatment efficacy and the survival of iPSC-CMs in vivo after transplantation have yielded inconsistent results. OBJECTIVES: The objective of this study was to evaluate the ability of human iPSC-CMs to improve myocardial function in a rat postinfarction heart failure model. METHODS: Eight-week-old male Sprague-Dawley rats were randomly selected to receive an intramyocardial injection of 5% albumin solution with or without 1 × 107 human iPSC-CMs 10 days after undergoing left anterior descending (LAD) coronary artery ligation. Cyclosporine A and methylprednisolone were administered before iPSC-CM injection and until the rats were killed to prevent graft rejection. Cardiac function was evaluated by echocardiography. The survival of grafted cardiomyocytes was confirmed by observing the fluorescent cell tracer Vybrant™ CM-DiI or expression of the enhanced green fluorescent protein (eGFP) in transplanted cells, or survival was demonstrated by polymerase chain reaction (PCR)-based detection of human mitochondrial DNA. Sirius red stain was used to evaluate the fibrosis ratio. Hematoxylin-eosin staining was used to observe the formation of teratomas. RESULTS: Four weeks after intramyocardial injection of iPSC-CMs, animals undergoing iPSC-CM transplantation had lower mortality than the control group. Animals injected with cell-free solution (control group) demonstrated significant left ventricular (LV) functional deterioration, whereas grafting of iPSC-CMs attenuated this remodeling process. In the control group, the ejection fraction deteriorated by 10.11% (from 46.36 to 41.67%), and fractional shortening deteriorated by 9.23% (from 24.37 to 22.12%) by 4 weeks. In the iPSC-CM injection group, the ejection fraction improved by 18.86% (from 44.09 to 52.41%), and fractional shortening improved by 23.69% (from 23.08 to 28.54%). Cell labeling, tracking, and molecular biology techniques indicated that the grafted cardiomyocytes survived in the rat heart 1 month after iPSC-CM transplantation. Myocardial fibrosis was also attenuated in the iPSC-CM treatment group. CONCLUSIONS: Human iPSC-CM grafts survived in infarcted rat hearts and restored myocardial function 4 weeks after transplantation. Cell replacement therapy also reversed ventricular remodeling, indicating the potential of iPSC-CMs for cardiac repair strategies.

Mechanical characteristics of medical grade UHMWPE under dynamic compression.

The mechanical properties of medical grade ultrahigh molecular weight polyethylene (UHMWPE) are critical for the safety and integrity of UHMWPE implantation. Accordingly, the mechanical features of UHMWPE are tested under repeated stress-controlled and strain-controlled compression at room temperature. Some important effect factors, such as stress rate, mean stress, stress amplitude, strain rate, mean strain, strain range and multiple load steps are further considered in detail. Results indicate that the lower stress rate causes the greater accumulated plastic strain and the accumulated plastic strain rate becomes increasingly lower with increasing number of cycles. The strain range and accumulated plastic strain rate decrease rapidly in the first stage, and then become almost steady during the second stage. Especially, the accumulated plastic strain rate per cycle for each case is less than 0.01 %/cycle after the initial 100 cycles. This means that the plastic strain accumulates very slowly and the shakedown behavior always occurs. Moreover, obvious cyclic softening and stress relaxation behaviors can be observed under cyclic strain-controlled compression during the first 50 cycles. This indicates that the accumulated plastic stain in the initial 100 cycles and the cyclic stress relaxation during the first 50 cycles should be assessed for the functionality of UHMWPE implantation.

A single mutation in the prM protein of Zika virus contributes to fetal microcephaly.

Zika virus (ZIKV) has evolved into a global health threat because of its unexpected causal link to microcephaly. Phylogenetic analysis reveals that contemporary epidemic strains have accumulated multiple substitutions from their Asian ancestor. Here we show that a single serine-to-asparagine substitution [Ser139→Asn139 (S139N)] in the viral polyprotein substantially increased ZIKV infectivity in both human and mouse neural progenitor cells (NPCs) and led to more severe microcephaly in the mouse fetus, as well as higher mortality rates in neonatal mice. Evolutionary analysis indicates that the S139N substitution arose before the 2013 outbreak in French Polynesia and has been stably maintained during subsequent spread to the Americas. This functional adaption makes ZIKV more virulent to human NPCs, thus contributing to the increased incidence of microcephaly in recent ZIKV epidemics.

Immunization with truncated envelope protein of Zika virus induces protective immune response in mice.

The global spread of Zika virus (ZIKV) as well as its unexpected link to infant microcephaly have resulted in serious public health concerns. No antiviral drugs against ZIKV is currently available, and vaccine development is of high priority to prepare for potential ZIKV pandemic. In the present study, a truncated E protein with the N-terminal 90% region reserved (E90) from a contemporary ZIKV strain was cloned and expressed in Escherichia coli, purified by a Ni-NTA column, and characterized by Western blotting assays. Immunization with recombinant E90 induced robust ZIKV-specific humoral response in adult BALB/c mice. Passive transfer of the antisera from E90-immunized mice conferred full protection against lethal ZIKV challenge in a neonatal mice model. Our results indicate that recombinant ZIKV E90 described here represents as a promising ZIKV subunit vaccine that deserves further clinical development.

Characterization of cis-Acting RNA Elements of Zika Virus by Using a Self-Splicing Ribozyme-Dependent Infectious Clone.

Zika virus (ZIKV) has caused significant outbreaks and epidemics in the Americas recently, raising global concern due to its ability to cause microcephaly and other neurological complications. A stable and efficient infectious clone of ZIKV is urgently needed. However, the instability and toxicity of flavivirus cDNA clones in Escherichia coli hosts has hindered the development of ZIKV infectious clones. Here, using a novel self-splicing ribozyme-based strategy, we generated a stable infectious cDNA clone of a contemporary ZIKV strain imported from Venezuela to China in 2016. The constructed clone contained a modified version of the group II self-splicing intron P.li.LSUI2 near the junction between the E and NS1 genes, which were removed from the RNA transcripts by an easy-to-establish in vitro splicing reaction. Transfection of the spliced RNAs into BHK-21 cells led to the production of infectious progeny virus that resembled the parental virus. Finally, potential cis-acting RNA elements in ZIKV genomic RNA were identified based on this novel reverse genetics system, and the critical role of 5'-SLA promoter and 5'-3' cyclization sequences were characterized by a combination of different assays. Our results provide another stable and reliable reverse genetics system for ZIKV that will help study ZIKV infection and pathogenesis, and the novel self-splicing intron-based strategy could be further expanded for the construction of infectious clones from other emerging and reemerging flaviviruses.IMPORTANCE The ongoing Zika virus (ZIKV) outbreaks have drawn global concern due to the unexpected causal link to fetus microcephaly and other severe neurological complications. The infectious cDNA clones of ZIKV are critical for the research community to study the virus, understand the disease, and inform vaccine design and antiviral screening. A panel of existing technologies have been utilized to develop ZIKV infectious clones. Here, we successfully generated a stable infectious clone of a 2016 ZIKV strain using a novel self-splicing ribozyme-based technology that abolished the potential toxicity of ZIKV cDNA clones to the E. coli host. Moreover, two crucial cis-acting replication elements (5'-SLA and 5'-CS) of ZIKV were first identified using this novel reverse genetics system. This novel self-splicing ribozyme-based reverse genetics platform will be widely utilized in future ZIKV studies and provide insight for the development of infectious clones of other emerging viruses.

Viral RNA switch mediates the dynamic control of flavivirus replicase recruitment by genome cyclization.

Viral replicase recruitment and long-range RNA interactions are essential for RNA virus replication, yet the mechanism of their interplay remains elusive. Flaviviruses include numerous important human pathogens, e.g., dengue virus (DENV) and Zika virus (ZIKV). Here, we revealed a highly conserved, conformation-tunable cis-acting element named 5'-UAR-flanking stem (UFS) in the flavivirus genomic 5' terminus. We demonstrated that the UFS was critical for efficient NS5 recruitment and viral RNA synthesis in different flaviviruses. Interestingly, stabilization of the DENV UFS impaired both genome cyclization and vRNA replication. Moreover, the UFS unwound in response to genome cyclization, leading to the decreased affinity of NS5 for the viral 5' end. Thus, we propose that the UFS is switched by genome cyclization to regulate dynamic RdRp binding for vRNA replication. This study demonstrates that the UFS enables communication between flavivirus genome cyclization and RdRp recruitment, highlighting the presence of switch-like mechanisms among RNA viruses.