The influence of in situ purification system on pathogen in the river fed by the drainage of sewage plant.

An in situ integrated system, consisting of ecological floating islands (EFI), ecological riverbeds (ER), and ecological filter dams (EFD), was built in a ditch only receiving the effluent of sewage plant; the effect of in situ technologies on the distribution of aquatic pathogen was investigated. The results showed the aquatic pathogen decreased along the ditch. Specifically, the relative abundance of Legionella, Aeromonas, and Acinetobacter decreased from 0.032, 0.035, and 0.26 to 0.026%, 0.012%, and 0.08%, respectively. Sedimentation, filtration, and sorption (provided by plant roots and biofilms on substrates) were principal processes for the removal. The nitrogen removal bacteria to prevent the potential risk of eutrophication were also evaluated. The EFI and ER were the dominant sites for Nitrosomonas (34.96%, 32.84%) and Nitrospira (35.74%, 54.73%) enrichment, while EFI and EFD facilitated the enrichment of denitrification bacteria. Notably, the relative abundance of endogenous denitrifiers (DNB-en) (including Dechloromonas at 9.72%, Thermomonas at 0.58%, and Saccharibacteria at 2.55%) exceeded those of exogenous denitrifiers (DNB-ex) (Thauera at 0.20%, Staphylococcus at 0.005%, and Rhodobacter at 0.27%). This study demonstrated that the in situ integrated system was effective in reducing the abundance of pathogens in the drainage channel, and the deficiency of DNB-ex and carbon sources made nitrate removal difficult.

Comparison of agricultural wastes and synthetic macromolecules as solid carbon source in treating low carbon nitrogen wastewater.

This paper investigated the feasibility of using agricultural wastes and synthetic macromolecules as solid carbon sources and studied the effects of improvement of denitrification by the selected agricultural wastes. The carbon release capacity and denitrification performance of corncob (CC), peanut shell (PS), obsolescent rice (OR) and polycaprolactone (PCL), poly butylene succinate (PBS), polyvinyl alcohol sodium alginate (PVA-SA) were systematically analyzed. The results showed that for each carbon source, the first-order kinetic equation was basically followed during the carbon release process. PVA-SA, CC and PS had higher carbon release capacity with accumulative dissolved organic carbon (DOC) of 16.22-20.63 mg·g-1 and chemical oxygen demand (COD) of 100.86-134.10 mg·g-1. Correspondingly, they showed excellent denitrification performance with almost no residual NO3--N, and the denitrification process well followed the Monod equation. PCL, PBS and OR had lower carbon release capacity with accumulative DOC of 2.06-3.14 mg·g-1 and COD of 13.29-24.13 mg·g-1, respectively. Nevertheless, these materials can also improve the denitrification performance, with the residual NO3--N in the range of 6.02-6.36 mg·L-1, and the effluent DOC was in the range of 10-15 mg·L-1. Synthetic polymers are more suitable for nitrogen removal in groundwater treatment, while agricultural wastes are ideal carbon sources for secondary effluent treatment.

Toxicological Assessment of Ammonia Exposure on Carassius auratus red var. Living in Landscape Waters.

To understand the toxic mechanism of ammonia and identify effective biomarkers on the oxidative stress for the fish Carassius auratus red var., acute and chronic toxicity tests were conducted. The 96-h LC50 of total ammonia nitrogen (TAN) for C. auratus was 135.4 mg L-1, the corresponding unionized ammonia (NH3) concentration was 1.5 mg L-1. The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione-peroxidase (GSH-Px) and glutathione (GSH) showed an increase with a subsequent falling, while the malondialdehyde (MDA) increased during the chronic test. The SOD, MDA, and GSH could be effective biomarkers to evaluate the TAN oxidative stress, the maximum acceptable toxicant concentration (MATC) was 11.3 mg L-1 for TAN. To our knowledge, this is the first study to propose biomarkers to evaluate potential environmental risk and establish a risk threshold for TAN in C. auratus.

Biological denitrification using polycaprolactone-peanut shell as slow-release carbon source treating drainage of municipal WWTP.

The development of slow-release carbon source is an effective way to reduce the total nitrogen (TN) in low carbon to nitrogen ratio wastewater. In this study, a novel solid slow-release carbon source (PPP) was prepared using polycaprolactone (PCL) and peanut shell (PS) as carbon sources with polyvinyl alcohol-sodium alginate (PVA-SA) as hybrid scaffolds. The carbon release properties of PPP and each carbon source materials were compared. The performances of nitrogen removal and microbial community structure using PPP as external carbon source were investigated. The results showed that PPP had the best slow-release performance, and its release process followed the first-order release equation. The ratio of acetic acid, propionic acid and butyric acid in released organic matter was stable at (75.73 ±â€¯4.62)%:(17.22 ±â€¯4.53)%:(7.06 ±â€¯1.02)%. When using PPP as an external carbon source for denitrification, the relative abundance of Gammaproteobacteria increased from 39.32% to 46.66%, while the Shannon index decreased from 8.59 to 8.29. The utilization efficiency of PPP was determined by the ratio of the organic matter releasing rate to the released organic matter consumption rate. By optimizing the PPP dosage, both high nitrogen removal efficiency and low residual organic matter could be achieved.

Effect of corn cobs as external carbon sources on nitrogen removal in constructed wetlands treating micro-polluted river water.

Micro-polluted river water is characterized as having limited biodegradability, low carbon to nitrogen ratio and little organic carbon supply, all of which makes it hard to further purify. Two bench scale constructed wetlands (CWs) with a horizontal subsurface flow mode were set up in the laboratory to evaluate their feasibility and efficiency on denitrification with and without corn cobs as external carbon sources. Micro-polluted river water was used as feed solution. The CW without corn cobs substrates possessed a good performance in removing chemical oxygen demand (COD, <40 mg/L) and ammonia nitrogen (NH3-N, <0.65 mg/L), but less efficiency in removing total nitrogen (TN) and nitrate nitrogen (NO3-N). In marked contrast, the CW with 1% (w/w) corn cobs substrates as external carbon sources achieved a significant improvement in the removal efficiency of TN (increased from 34.2% to 71.9%) and NO3-N (increased from 19% to 71.9%). The incorporation of corn cobs substrates did not cause any obvious increase in the concentrations of COD and NH3-N in the effluent. This improvement in the denitrification efficiency was owing to the released organic carbon from corn cobs substrates, which facilitated the growth of abundant microbes on the surface and pores of the substrate. The open area of the used corn chips is larger than that of the pristine ones, and corn cobs can continue to provide a carbon fiber source for denitrification.

Control of sludge settleability based on organic load and ammonia nitrogen load under low dissolved oxygen.

Controlling dissolved oxygen (DO) at low level can save energy for wastewater treatment plants (WWTPs), but it is easy to induce filamentous sludge bulking. Through establishing the kinetic equation of sludge settleability, ammonia nitrogen (NH4 +-N) load and organic load (food-to-microbe ratio, F/M), the mechanism of the competitive relationship between filamentous and floccular bacteria under low DO was analyzed. The results showed when DO, NH4 +-N load and F/M were in the range of 0.15-0.35 mg/L, 0.035-0.15 d-1 and 0.12-0.42 d-1, respectively, the mass transfer limitation of organic matter was the main factor determining the dominant growth of filamentous bacteria. When DO, NH4 +-N load and F/M were in the range of 0.35-0.65 mg/L, 0.035-0.065 d-1 and 0.12-0.22 d-1, respectively, the mass transfer limitation of NH4 +-N was the main factor determining the dominant growth of filamentous bacteria. When DO was low, no matter how NH4 +-N load and F/M changed, the growth of filamentous bacteria was promoted. When DO and F/M were in the range of 0.35-0.65 mg/L and 0.22-0.42 d-1, respectively, no matter how NH4 +-N load and F/M changed, the growth of filamentous bacteria was inhibited. Therefore, in actual operation, ensuring relatively low DO and high F/M was beneficial for the sludge settleability improvement.

Ammonium removal by native microbes and activated sludge within the Jialu River basin and the associated microbial community structures.

To explore the availability of native microbes and activated sludge for ammonium removal, the native microbes and activated sludge in Jialu River basin were investigated in terms of ammonium-removing activities and their microbial communities using spectrophotometry and high-throughput sequencing. NH4+-N and total nitrogen (TN) in the targeted river ranged from 2.45 ± 1.76 to 8.56 ± 2.54 mg/L and from 3.42 ± 2.79 to 13.49 ± 5.06 mg/L, respectively. Both the native microbes and activated sludge had strong ammonium-removing activities with the removal efficiencies of more than 94%. High-throughput sequencing results indicated that, after five batches of operation, the class Gammaproteobacteria (28.55%), Alphaproteobacteria (14.55%), Betaproteobacteria (13.89%), Acidobacteria (8.82%) and Bacilli (7.04%) were dominated in native community, and there was a predominance of Gammaproteobacteria (21.57%), Betaproteobacteria (16.33%), Acidobacteria (12.41%), Alphaproteobacteria (10.01%), Sphingobacteriia (6.92%) and Bacilli (6.66%) in activated sludge. These two microbial sources were able to remove ammonium, while activated sludge was more cost-effective.

Phosphate adsorption and precipitation on calcite under calco-carbonic equilibrium condition.

Phosphate (PO43-) removal on calcite often entails two processes: adsorption and precipitation. Separating these two processes is of great importance for assessment of PO43- stability after removal. Thus, this study was aimed at finding a critical range of conditions for separating these two processes in calco-carbonic equilibrium, by adjusting PO43- concentration, reaction time and pH. PO43- removal kinetic results showed that: (I) At pH7.7, PO43- removal was mainly by adsorption at initial PO43- concentration ≤2.2 mg L-1 and reaction time ≤24 h, with dominant precipitation occurring at initial PO43- concentration ≥3 mg L-1 after 24 h reaction; (II) At pH8.3, adsorption was the key removal process at initial PO43- concentration ≤7.5 mg L-1 and reaction time ≤24 h, whereas precipitation was observed at initial PO43- concentration of 10 mg L-1 after 24 h reaction, (III) At pH 9.1 and 10.1, PO43- removal mechanism was mainly by adsorption at initial PO43- concentration ≤10 mg L-1 within 24 h reaction. Based on the kinetic results, it is suggested that PO43- precipitation will occur after 24 h reaction when saturation index of amorphous calcium phosphate is between 1.97 and 2.19. Besides, increasing PO43- concentration does not cause a continuous decline of PO43- removal percentage. Moreover, experimental removal data deviated largely from the theoretical adsorption value by CD-MUSIC model. These indicate occurrence of precipitation which is in agreement with the kinetic result. Therefore our study will provide fundamental reference information for better understanding of phosphorous stabilization after removal by calcite.

Polycyclic aromatic hydrocarbons and ecotoxicological characterization of sediments from the Huaihe River, China.

The distribution, source, ecological risk and ecotoxicity of polycyclic aromatic hydrocarbons (PAHs) of sediments from 7 sampling sites, named as Xinyang (XY), Huainan (HN), Bengbu (BB), Xuyi (XuY), Fuyang (FY), Mengcheng (MC) and Zhengzhou (ZZ), in the Huaihe River basin, China, have been investigated. The total concentrations of 16 USEPA priority PAHs ranged from 62.9 to 2232.4 ng g⁻¹ dry weight (d.w.) with a mean concentration of 1056.8 ng g⁻¹ d.w. Through the assessment of ecological risk, we found that the levels of PAHs in the Huaihe River should not exert adverse biological effects. The total benzo[a]pyrene toxicity equivalent (TEQ) values calculated for samples varied from 0.01 to 194.1 ng g⁻¹ d.w., with an average of 65.9 ng g⁻¹. The toxicity data were accordant with the chemical analysis results in this study. HN, BB and ZZ showed the greatest pollution extent both in the chemical analysis and the study of ecotoxicological effects.