ABSTRACT
In urban intersections, the sensory capabilities of autonomous vehicles (AVs) are often hindered by visual obstructions, posing significant challenges to their robust and safe operation. This paper presents an implementation study focused on enhancing the safety and robustness of Connected Automated Vehicles (CAVs) in scenarios with occluded visibility at urban intersections. A novel LiDAR Infrastructure System is established for roadside sensing, combined with Baidu Apollo's Automated Driving System (ADS) and Cohda Wireless V2X communication hardware, and an integrated platform is established for roadside perception enhancement in autonomous driving. The field tests were conducted at the Singapore CETRAN (Centre of Excellence for Testing & Research of Autonomous Vehicles-NTU) autonomous vehicle test track, with the communication protocol adhering to SAE J2735 V2X communication standards. Communication latency and packet delivery ratio were analyzed as the evaluation metrics. The test results showed that the system can help CAV detect obstacles in advance under urban occluded scenarios.
ABSTRACT
DNA can be used to store digital data, and synthetic short-sequence DNA pools are developed to store high quantities of digital data. However, synthetic DNA data cannot be actively processed in DNA pools. An active DNA data editing process is developed using splint ligation in a droplet-controlled fluidics (DCF) system. DNA fragments of discrete sizes (100-500 bps) are synthesized for droplet assembly, and programmed sequence information exchange occurred. The encoded DNA sequences are processed in series and parallel to synthesize the determined DNA pools, enabling random access using polymerase chain reaction amplification. The sequencing results of the assembled DNA data pools can be orderly aligned for decoding and have high fidelity through address primer scanning. Furthermore, eight 90 bps DNA pools with pixel information (png: 0.27-0.28 kB), encoded by codons, are synthesized to create eight 270 bps DNA pools with an animation movie chip file (mp4: 12 kB) in the DCF system.
Subject(s)
DNA , DNA/genetics , Polymerase Chain Reaction/methodsABSTRACT
As molecular computing materials, information-encoded deoxyribonucleic acid (DNA) strands provide a logical computing process by cascaded and parallel chain reactions. However, the reactions in DNA-based combinational logic computing are mostly achieved through a manual process by adding desired DNA molecules in a single microtube or a substrate. For DNA-based Boolean logic, using microfluidic chips can afford automated operation, programmable control, and seamless combinational logic operation, similar to electronic microprocessors. In this paper, we present a programmable DNA-based microfluidic processing unit (MPU) chip that can be controlled via a personal computer for performing DNA calculations. To fabricate this DNA-based MPU, polydimethylsiloxane was cast using double-sided molding techniques for alignment between the microfluidics and valve switch. For a uniform surface, molds fabricated using a three-dimensional printer were spin-coated by a polymer. For programming control, the valve switch arms were operated by servo motors. In the MPU controlled via a personal computer or smartphone application, the molecules with two input DNAs and a logic template DNA were reacted for the basic AND and OR operations. Furthermore, the DNA molecules reacted in a cascading manner for combinational AND and OR operations. Finally, we demonstrated a 2-to-1 multiplexer and the XOR operation with a three-step cascade reaction using the simple DNA-based MPU, which can perform Boolean logic operations (AND, OR, and NOT). Through logic combination, this DNA-based Boolean logic MPU, which can be operated using programming language, is expected to facilitate the development of complex functional circuits such as arithmetic logical units and neuromorphic circuits.
