ABSTRACT
Genomic DNA isolation is a crucial technique for researchers studying plant molecular biology. A current widely-used protocol for DNA extraction needs a pestle and mortal for each sample and consumes a large amount of liquid nitrogen in grinding the samples. Most high-throughput methods depend on expensive machines for tissue homogenization. Here we developed a CTAB-based DNA extraction method using 2.0â¯mL microcentrifuge tubes for sample processing. This protocol has the advantages that it is suitable for a variety of plants, easily-performed without special equipment, and high-throughput; it effectively avoids sample cross-contamination, and is inexpensive, rapid and safe.
Subject(s)
DNA, Plant/isolation & purification , Genome, Plant , High-Throughput Screening Assays/methods , Plants/genetics , Arabidopsis/genetics , DNA, Plant/analysis , Electrophoresis, Agar Gel , Liquid-Liquid ExtractionABSTRACT
In the title compound, C(14)H(15)NO(2), the dihedral angle between the two benzene rings is 71.10â (5)°. In the crystal, mol-ecules are linked by inter-molecular N-Hâ¯O, and O-Hâ¯N hydrogen bonds into a chain running parallel to the b axis.
