ABSTRACT
We investigated the effects of tonsillectomy by low-temperature plasma on the growth development and psychological behavior in children with obstructive sleep apnea hypopnea syndrome (OSAHS).This study included 72 moderate and severe OSAHS children with tonsils or adenoids hypertrophy, which were randomly assigned into either the study group (nâ=â36) or the control group (nâ=â36). Patients in study group underwent tonsillectomy by low-temperature plasma, while in the control group underwent tonsil-pecking, then the efficacy were compared.The time of surgery, VAS scores on postoperative day 1, 3, and 7 were significantly lower in the study group than in the control group, and the efficiency was significantly higher in the study group than the control group (Pâ=â.018). In the study group, the BMI was lower, the score of C-WISC (VIQ, PIQ, and FIQ) was higher, the score of CBCL social competence was higher and the score of behavioral questions was lower than that in the control group; differences were statistically significant (Pâ=â.022). The serum levels of IgA, IgG, and IgM, as well as the percentage of T lymphocytes, between the study group and the control group were not significantly different (Pâ=â.132).Tonsillectomy by low-temperature plasma was effective on the treatment of children with severe OSAHS, and could improve growth development and psychological behavior.
Subject(s)
Argon Plasma Coagulation/methods , Child Behavior/physiology , Child Development , Sleep Apnea, Obstructive , Tonsillectomy/methods , Adenoids/pathology , Adenoids/surgery , Child , Child, Preschool , Female , Humans , Male , Palatine Tonsil/pathology , Palatine Tonsil/surgery , Polysomnography/methods , Postoperative Period , Republic of Korea , Sleep Apnea, Obstructive/diagnosis , Sleep Apnea, Obstructive/physiopathology , Sleep Apnea, Obstructive/psychology , Treatment OutcomeABSTRACT
BACKGROUND: Jumonji C domain 2A (JMJD2A), as a histone demethylases, plays a vital role in tumorigenesis and progression. But, its functions and underlying mechanisms of JMJD2A in nasopharyngeal carcinoma (NPC) metabolism are remained to be clarified. In this study, we investigated glycolysis regulation by JMJD2A in NPC and the possible mechanism. METHODS: JMJD2A expression was detected by Western blotting and Reverse transcription quantitative real-time PCR analysis. Then, we knocked down and ectopically expressed JMJD2A to detect changes in glycolytic enzymes. We also evaluated the impacts of JMJD2A-lactate dehydrogenase A (LDHA) signaling on NPC cell proliferation, migration and invasion. ChIP assays were used to test whether JMJD2A bound to the LDHA promoter. Finally, IHC was used to verify JMJD2A and LDHA expression in NPC tissue samples and analyze their correlation between expression and clinical features. RESULTS: JMJD2A was expressed at high levels in NPC tumor tissues and cell lines. Both JMJD2A and LDHA expression were positively correlated with the tumor stage, metastasis and clinical stage. Additionally, the level of JMJD2A was positively correlated with LDHA expression in NPC patients, and higher JMJD2A and LDHA expression predicted a worse prognosis. JMJD2A alteration did not influence most of glycolytic enzymes expression, with the exception of PFK-L, PGAM-1, LDHB and LDHA, and LDHA exhibited the greatest decrease in expression. JMJD2A silencing decreased LDHA expression and the intracellular ATP level and increased LDH activity, lactate production and glucose utilization, while JMJD2A overexpression produced the opposite results. Furthermore, JMJD2A could combine to LDHA promoter region and regulate LDHA expression at the level of transcription. Activated JMJD2A-LDHA signaling pathway promoted NPC cell proliferation, migration and invasion. CONCLUSIONS: JMJD2A regulated aerobic glycolysis by regulating LDHA expression. Therefore, the novel JMJD2A-LDHA signaling pathway could contribute to the Warburg effects in NPC progression.
Subject(s)
Carcinoma/genetics , Carcinoma/metabolism , Gene Expression Regulation, Neoplastic , Jumonji Domain-Containing Histone Demethylases/metabolism , L-Lactate Dehydrogenase/genetics , Nasopharyngeal Neoplasms/genetics , Nasopharyngeal Neoplasms/metabolism , Adenosine Triphosphate/metabolism , Adult , Aged , Biomarkers , Carcinoma/mortality , Carcinoma/pathology , Cell Line, Tumor , Cell Movement/genetics , Cell Proliferation , Disease Progression , Enzyme Activation , Female , Gene Expression , Genes, Reporter , Glucose/metabolism , Glycolysis , Humans , Isoenzymes/genetics , Isoenzymes/metabolism , Kaplan-Meier Estimate , L-Lactate Dehydrogenase/metabolism , Lactate Dehydrogenase 5 , Lactic Acid/metabolism , Male , Middle Aged , Nasopharyngeal Carcinoma , Nasopharyngeal Neoplasms/mortality , Nasopharyngeal Neoplasms/pathology , Neoplasm Grading , Neoplasm Staging , Signal Transduction , Transcription, GeneticABSTRACT
Sensory gating, a viable function of the brain, is an adaptive mechanism to prevent overstimulation of nervous system. The aim of this study was to examine the effect of homobaric pure (i.e. 100%) oxygen on the human brain at different periods of inhalation. EEG was recorded while an auditory paired-click sensory gating test was conducted during 4 study periods: before inhalation of pure oxygen (Before), inhalation of 100% oxygen (air in control group) for 20 min (Oxy20) and 50 min (Oxy50), 30 min after oxygen (air in control group) inhalation (After). Each of the auditory stimuli elicited 4 clear peaks at 20, 39, 55 and 100 ms in ERPs, demonstrating that sensory gating is a multi-stage process. Comparing the S1-S2 differences of field potentials between two groups, significant experimental effects (P < 0.05-0.01) were shown at Oxy50 and After periods mainly at the 20 and 100 ms peak in ERPs. Pure oxygen was experimentally shown, for the first time, to affect the human brain activation, at the beginning of early P20 sensory cortical activation and late N100 auditory perception. The effect found in this study shall encourage further investigation on the oxygen treatment in human brain.
