Effects of Different Nursing Modes on Immune Function and Renal Function in Patients with Renal Calculus Undergoing Percutaneous Nephrolithotomy.

BACKGROUND: This study aimed to explore the effects of different nursing modes on immune function and renal function in patients with renal calculus and on percutaneous nephrolithotomy (PCNL). METHODS: A total of 160 patients with kidney stone who were admitted to our hospital from January 2017 to January 2023 were retrospectively selected and equally divided into routine and comprehensive nursing groups. Both groups were treated with percutaneous nephrolithotomy, but the patients in the routine nursing group were treated with usual care, whereas the patients in the comprehensive nursing group were treated with comprehensive nursing. The levels of serum T lymphocyte subsets were detected by flow cytometry, the levels of serum immune indicators and renal function indicators in the two groups were measured and the incidence of postoperative complications and nursing satisfaction were recorded. RESULTS: The levels of serum blood urea nitrogen, creatinine, kidney injury molecule and CD8+ cell subsets in the comprehensive nursing group were lower than those in the routine nursing group (p < 0.05), whereas the CD4+ cell subsets, CD4+:CD8+ ratio, and immunoglobulins (Ig)A, IgG and IgM levels were significantly higher than those in the routine nursing group (p < 0.05). The incidence of postoperative complications in the comprehensive nursing group was 2.50% (2/80), which was significantly lower than that in the routine nursing group (13.75%, 11/80), and the difference was statistically significant (χ2 = 6.782, p = 0.009). Nursing satisfaction in the comprehensive nursing group was 96.25% (77/80), which was significantly higher than that in the routine nursing group (85.00%, 68/80; χ2 = 5.959, p = 0.015). CONCLUSIONS: The comprehensive nursing mode can effectively reduce the influence of PCNL on T cell subsets, immunoglobulin levels in patients with renal calculus, damage to renal function and complications; Can improve patients' satisfaction with nursing; And promote postoperative recovery.

Effects of Different Nursing Modes on Immune Function and Renal Function in Patients with Renal Calculus Undergoing Percutaneous Nephrolithotomy

Background: This study aimed to explore the effects of different nursing modes on immune function and renal function in patients with renal calculus and on percutaneous nephrolithotomy (PCNL). Methods: A total of 160 patients with kidney stone who were admitted to our hospital from January 2017 to January 2023 were retrospectively selected and equally divided into routine and comprehensive nursing groups. Both groups were treated with percutaneous nephrolithotomy, but the patients in the routine nursing group were treated with usual care, whereas the patients in the comprehensive nursing group were treated with comprehensive nursing. The levels of serum T lymphocyte subsets were detected by flow cytometry, the levels of serum immune indicators and renal function indicators in the two groups were measured and the incidence of postoperative complications and nursing satisfaction were recorded. Results: The levels of serum blood urea nitrogen, creatinine, kidney injury molecule and CD8+ cell subsets in the comprehensive nursing group were lower than those in the routine nursing group (p < 0.05), whereas the CD4+ cell subsets, CD4+:CD8+ ratio, and immunoglobulins (Ig)A, IgG and IgM levels were significantly higher than those in the routine nursing group (p < 0.05). The incidence of postoperative complications in the comprehensive nursing group was 2.50% (2/80), which was significantly lower than that in the routine nursing group (13.75%, 11/80), and the difference was statistically significant (χ2 = 6.782, p = 0.009). Nursing satisfaction in the comprehensive nursing group was 96.25% (77/80), which was significantly higher than that in the routine nursing group (85.00%, 68/80; χ2 = 5.959, p = 0.015) (AU)

High-intensity focused ultrasound treatment for symptomatic uterine fibroids: a systematic review and meta-analysis.

OBJECTIVE: This study was aimed at comparing the outcomes of high-intensity focused ultrasound (HIFU) with those of uterine artery embolization (UAE) and traditional surgeries for treating symptomatic uterine fibroids. MATERIALS AND METHODS: We searched the following databases from their beginning to 5 November 2021: PubMed, Medline, Embase and Cochrane Library. RESULTS: Overall, 21 studies were included in this meta-analysis. The results revealed that HIFU had a higher re-intervention rate than UAE (relative risk [RR] = 4.06, 95% confidence interval [CI]: 2.47-6.69) and offered no significant advantages in reducing the symptom severity score (SSS) (mean difference [MD] = 17.01, 95% CI: 10.25-23.77) and improving the health-related quality of life (HRQoL) score (MD= -18.32, 95% CI: -24.87 to -11.78) in the treatment of symptomatic uterine fibroids. However, compared with UAE, HIFU may be associated with a higher pregnancy rate (RR = 17.44, 95% CI: 2.40-126.50) and may have a significant advantage in shortening pregnancy interval and preserving ovarian function. Moreover, upon comparing HIFU with traditional surgical treatments, the HIFU group showed significantly improved HRQoL score (MD = 2.25, 95% CI: 1.15-3.35), but the re-intervention rate (RR = 1.65, 95% CI: 0.59-4.57), pregnancy rate (RR = 1.01, 95% CI: 0.90-1.13), SSS and ovarian function did not significantly differ between the two groups. CONCLUSIONS: Although HIFU has relatively high re-intervention rate, it may offer a higher pregnancy rate and shorter pregnancy interval with little influence on ovarian function, thus making it an attractive option for treating symptomatic fibroids in young women who wish to plan a pregnancy in the future.

Analysis of STAG3 variants in Chinese non-obstructive azoospermia patients with germ cell maturation arrest.

STAG3 is essential for male meiosis and testis of male Stag3-/- mice shows the histopathological type of germ cell maturation arrest (MA). Whether variants of the STAG3 gene exist in Chinese idiopathic non-obstructive azoospermia (NOA) patients needs to be determined. We recruited 58 Chinese NOA men with MA who underwent testis biopsy and 192 fertile men as the control group. The 34 exons of the STAG3 gene were amplified using polymerase chain reaction (PCR) and sequenced. We identified eight novel single nucleotide polymorphisms (SNPs), including two missense SNPs (c.433T > C in exon2 and c.553A > G in exon3), three synonymous SNPs (c.539G > A, c.569C > T in exon3, and c.1176C > G in exon8), and three SNPs in introns. The allele and genotype frequencies of the novel and other SNPs have no significant differences between two groups. Our results indicated that variants in the coding sequence of the STAG3 gene were uncommon in NOA patients with MA in Chinese population. Future studies in large cohorts of different ethnic populations will be needed to determine the association between the STAG3 gene and NOA.

Analysis of CDK2 mutations in Chinese men with non-obstructive azoospermia who underwent testis biopsy.

To examine whether mutations of the CDK2 gene exist in Chinese men with non-obstructive azoospermia (NOA) with different histopathology, we recruited 175 Chinese men with idiopathic NOA who underwent testis biopsy, including hypospermatogenesis, germ cell maturation arrest and Sertoli cell only syndrome. Genomic DNA was extracted from peripheral blood samples. Subsequently, the seven exons of the CDK2 gene were amplified using polymerase chain reaction with specific primers, respectively. The polymerase chain reaction products were sequenced on an automated sequencer. We identified four known single nucleotide polymorphisms: c.324G>A in exon 1; c.363T>C in exon 2; c.*570G>A; and c.*1160G>C in the 3' UTR of the CDK2 gene. Comparison of the genotype and allele frequencies showed no significant differences between NOA cases and controls for the four single nucleotide polymorphisms. Furthermore, no significant differences were found between each pathological group and control group, respectively. The results indicate that mutations in the coding sequence of the CDK2 gene may not be responsible for idiopathic NOA in Chinese men. Future studies in large cohorts of different ethnic populations are warranted to establish whether associations exist between the CDK2 gene and NOA.