Model-free robust motion control for biological optical microscopy using time-delay estimation with an adaptive RBFNN compensator.

The field of large numerical aperture microscopy has witnessed significant advancements in spatial and temporal resolution, as well as improvements in optical microscope imaging quality. However, these advancements have concurrently raised the demand for enhanced precision, extended range, and increased load-bearing capacity in objective motion carrier (OMC). To address this challenge, this study introduces an innovative OMC that employs a ball screw mechanism as its primary driving component. Furthermore, a robust nonlinear motion control strategy has been developed, which integrates fast nonsingular terminal sliding mode, experimental estimation techniques, and adaptive radial basis neural network, to mitigate the impact of nonlinear friction within the ball screw mechanism on motion precision. The stability of the closed-loop control system has been rigorously demonstrated through Lyapunov theory. Compared with other enhanced sliding mode control strategies, the maximum error and root mean square error of this controller are improved by 33% and 34% respectively. The implementation of the novel OMC has enabled the establishment of a high-resolution bio-optical microscope, which has proven its effectiveness in the microscopic imaging of retinal organoids.

Composite proportional-integral sliding mode control with feedforward control for cell puncture mechanism with piezoelectric actuation.

This paper presents a novel control strategy to compensate hysteretic nonlinearity and achieve precise positioning control of a cell puncture mechanism driven by a piezoelectric actuator (PEA). A dynamic model of the cell puncture mechanism is developed based on the Bouc-Wen model. Parameters of the nonlinear model are identified by particle swarm optimization. The strategy of feedforward (FF) control and sliding mode feedback (FB) control based on the Bouc-Wen inverse model is further developed to position the cell puncture mechanism. Zebrafish embryo is used as the validation object, wherein a cell micropuncture experiment is successfully performed. Proportional-integral sliding mode FB control plus FF control has a simple structure and exhibits excellent performance. Thus, this method can be easily extended to other micro-or nanopositioning mechanisms based on PEAs and adopted in practical applications.