ABSTRACT
AIM: To prepare and characterize the mouse monoclonal antibodies against human PON2 (paraoxonase 2). METHODS: A fragment of human PON2 gene of low homology with mice but of strong hydrophilicity and immunogenicity was selected for recombinant expression. Mice were immunized with the purified HIS fusion protein 3 times. The specificity and sensitivity of the anti-human PON2 monoclonal antibodies were characterized by Western blot and indirect immunofluorescence. RESULTS: HIS-PON2 and GST-PON2 fusion protein was highly expressed in E.coli with a molecular weight of 40 kDa and 46 kDa. Western blot analysis proved that the established monoclonal antibodies could specifically recognize the target proteins expressed in E.coli expression system. Indirect immunofluorescence analysis confirmed that PON2 protein was located in the cytoplasm of HepG2 cells. CONCLUSION: The monoclonal antibodies against human PON2 can specifically recognize natural protein expressed in human cells, Which can be used for further functional study of PON2 protein.
