[Clinical application of self-made drainage tubes in different layers of soft tissue for negative-pressure wound therapy in 33 patients].

From January 2014 to June 2018, 28 patients with different types of deep soft tissue injury or infection were admitted to the Affiliated Jiangyin Hospital of Medical College of Southeast University; 5 patients were admitted to the Zhengzhou First People's Hospital. There were 24 males and 9 females, aged 18-89 (40±20) years. Disposable suction tubes with holes cut on side walls were used as self-made drainage tubes. The authors placed the self-made drainage tubes on different deep soft tissue layers and wound surfaces after debridement. The effective drainage sections of the wound surface drainage tubes were wrapped with silver ion antimicrobial functional active dressings. Bio-permeable membrane was used to close the operative area. The drainage tubes in the deep layer of wound and wound surface were connected in parallel by a tee and connected to wall-hanging medical negative-pressure suction device to conduct negative-pressure wound treatment at -20.0 to -10.6 kPa. The deep drainage tubes were usually removed or changed 4 or 5 days after surgery.The drainage tubes in the wound surface were synchronously replaced when removing or replacing he drainage tubes in the deep layer of wound. On 4 to 15 days after surgery, the deep drainage tubes were removed. On 8 to 25 days after surgery, the wound surface drainage tubes were removed. Then the treatment was changed to a conventional dressing change until the wounds were completely healed or the wound bed was ready for skin grafts or tissue flaps. The indwelling time of deep drainage tubes in this group of patients was (6.2±2.8) days, and the indwelling time of wound surface drainage tubes was (12.0±3.0) days. The wound healing time was (22±5) days, the hospital stay time was (29±7) days, and wound bacteria were reduced from 6 species and 11 strains before treatment to 3 species and 4 strains after treatment. No adverse events such as wound bleeding, irritative pain, and chronic sinus occurred during treatment. Twenty-three patients were followed up for 13 to 28 months, no treatment-related complications were observed.

[Application of self-made vacuum sealing drainage device in postoperative fixation and drainage of abdominal pedicled flaps in 8 patients with deep burns of upper limbs].

From January 2013 to December 2017, 8 patients with deep burns of upper limbs were admitted to our hospital, including 6 males and 2 females, aged 23-48 years. The wound area of full-thickness burns to burns with tendon and bone injury was 4.5 cm×2.0 cm-20.0 cm×10.5 cm. After debridement, thin abdominal flaps with subdermal vascular network in the size of 5.0 cm×2.5 cm-22.0 cm×12.0 cm were applied to cover the wounds, and the donor sites were sutured directly by relaxation. The disposable suction tubes with holes cut on side walls were used as drainage tubes. The part of drainage tubes with holes were wrapped with nano-silver antimicrobial dressings and then placed at the lowest position of pedicle and donor site of abdominal flap and the space between the injured limb and the abdominal wall. The loose nano-silver antibacterial dressing was used to fill the webs of fingers and the gap between the injured limb and the abdominal wall. The transparent film dressing was used to close the surgical area and then connected with a low negative voltage electric suction device to continuously suck at a negative pressure of -15 to -10 kPa. The self-made vacuum sealing drainage device was replaced at intervals of 4 to 5 days until pedicle breakage was performed 2 to 3 weeks after operation. The pedicled abdominal flaps of 8 patients had no torsion or avulsion, no pedicle blood supply disorder, and no infection or skin erosion in the operation area, and all the flaps survived after pedicle breakage.

Characteristic analysis of pulmonary ground-glass lesions with the help of 64-slice CT technology.

OBJECTIVE: With the help of new technologies like 64-slice spiral CT, including latest AW4.4, 2D nodule comparing and analyzing technology, MPR and 3D technology, MIP technology and the technology of analyzing pulmonary vascular density by the method of perfusion scanning, we performed characteristic analysis of ground-glass opacities (GGO) for the early diagnosis of lung cancer. PATIENTS AND METHODS: We selected 62 patients suspected of lung cancer, whose conventional CT showed that they were patients with GGO. With the help of the new technologies of 64-slice spiral CT provided by GE Company, prospective scans were made and 2 to 4 times of review were arranged. After that, the patients were treated with surgery or needle biopsy to get lesion's pathological results. After several scans, the results including lesion's form, density, blood supply, peripheral sign, doubling time and tissue perfusion were drawn to make a comparison. Based on the results, comparative analysis on GGO's characteristics was made from morphological and functional perspectives. RESULTS: 41 patients (66.1%) were pathologically diagnosed with cancer, 10 were diagnosed with inflammation, 7 with fibrosis, and 4 with edema, hemorrhage and other lesions. The comparisons were made between the tumor groups' clinical manifestations (sex, age, symptoms including smoking, coughing, and expectoration), and the difference had no statistical significance (p>0.05). Conventional CT scan showed that the shape of GGO was irregular and it showed spiculated sign and pleural indentation. The proportion of the patients with vessel convergence in the tumor group was significantly higher than that of the non-tumor group (p<0.05). However, the comparisons between lesions' number, location (superior lobe of the right lung), diameter, edge (blur) and lobulation were made to get a difference ratio (p>0.05) which had no statistical significance. Tumor group's doubling time was significantly short, and its perfusion parameters including BF, BV, MTT, and PS were increased significantly (p<0.05). CONCLUSIONS: The new 64-slice CT technology has great value in the diagnosis of the tumorous GGO.

A modified uncorrelated linear discriminant analysis model coupled with recursive feature elimination for the prediction of bioactivity.

To meet the requirements of providing accurate, robust, and interpretable prediction of bioactivity, a modified uncorrelated linear discriminant analysis (M-ULDA) model was developed. In addition, a feature selection method called recursive feature elimination (RFE), originally used for support vector machine (SVM), was introduced and modified to fit the scheme of ULDA. From the evaluation of six pharmaceutical datasets, the M-UDLA coupled with RFE showed better or comparable classification accuracy with respect to other well-studied methods such as SVM and decision trees. The RFE used for ULDA has the advantage of increasing the computational speed and provides useful insights into biochemical mechanisms related to pharmaceutical activity by significantly reducing the number of variables used for the final model.

Expression of the water channel protein aquaporin-9 in human astrocytic tumours: correlation with pathological grade.

This study investigated the expression of the water channel protein aquaporin-9 (AQP9) in 100 cases of human astrocytic tumour compared with normal brain tissue. AQP9 mRNA and protein were assessed using reverse transcription-polymerase chain reaction and Western blot, respectively. The expression of both AQP9 mRNA and protein in astrocytic tumours was significantly greater than in normal brain tissue and was positively correlated with pathological grade. No significant correlations were found between the level of AQP9 mRNA or protein expression and gender, age, tumour size or tumour site. This study indicates that AQP9 may play an important role in the malignant progression of brain astrocytic tumours and may, therefore, be useful as a biomarker in its diagnosis and as a new target for gene therapy. The molecular mechanisms by which AQP9 affects the proliferation and apoptosis of astrocytic tumours need to be studied further.

Estradiol accelerates extinction of lithium chloride-induced conditioned taste aversions through its illness-associated properties.

Estradiol accelerates extinction of LiCl-induced conditioned taste aversions when it is present during a period that starts 2-3 days after acquisition and extends throughout extinction (before and during extinction). It has been suggested that estradiol acts before, not during, extinction and that its effect on extinction is associated with its illness-inducing properties. This hypothesis is based on previous work which shows an attenuation of conditioned taste aversion learning when rats are exposed to illness-inducing agents during a period that starts 2 days after acquisition and ends 2 days before extinction trials are initiated. Four experiments were designed to test elements of this hypothesis. The first two experiments demonstrated that if an estradiol-filled Silastic capsule is implanted before extinction of a LiCl-induced aversion, when the conditioned taste is not present, it accelerates extinction, but if it is implanted during extinction, when the conditioned taste is present, it prolongs extinction. The third experiment showed that the same dose of estradiol that accelerates extinction of a LiCl-induced aversion was effective in producing a conditioned taste aversion when it was present for 18 h after consumption of a novel sucrose solution. The fourth experiment indicated that serum levels of estradiol were elevated during the 18 h. These results are consistent with the hypothesis that the acceleration of extinction by estradiol is associated with its illness-inducing properties. It is suggested that estradiol acts on neural areas that mediate illness information and that one of these areas, the area postrema is necessary for estradiol to accelerate extinction of a LiCl-induced aversion.

Estradiol accelerates extinction of a conditioned taste aversion in female and male rats.

Exogenous testosterone treatment prolongs extinction of conditioned taste aversions and estradiol treatment prevents testosterone from prolonging extinction in both gonadectomized males and females. Estradiol could require the presence of testosterone for its effect or its action alone could accelerate extinction. The first series of experiments were designed to test the hypothesis that estradiol accelerates extinction when it is given in the absence of testosterone. The results showed that estradiol accelerates extinction of conditioned taste aversions in the absence of testosterone in gonadectomized Sprague-Dawley females and Fischer 344 females and males. The second series of experiments were designed to determine whether estradiol and testosterone differ in the temporal requirements for their opposite effects on extinction. The results showed that estradiol can accelerate extinction when it is present before and during acquisition (from 8 days before until 3 days after acquisition) or when it is present before and during extinction (from 2 days after acquisition, which was 23 days before extinction, until extinction trials were terminated). This is in contrast to a previous finding that testosterone prolongs extinction only when it is present before and during extinction. The following two hypotheses were suggested to account for the temporal effects of estradiol on extinction of conditioned taste aversions: (1) the presence of estradiol during acquisition reduces the effectiveness of LiCl through its action on the opioid system, and the presence of estradiol during extinction activates a neural pathway, such as that associated with activity levels, that accelerates extinction of passive avoidance tasks in general or (2) the presence of estradiol before, not during, acquisition or extinction accelerates extinction because of its illness-inducing properties. Most of the evidence supports the second hypothesis.

Blockage of the effects of testosterone on extinction of a conditioned taste aversion by estradiol: time of action.

Testosterone (T) prolongs the extinction of a conditioned taste aversion only if it is present during extinction. Experiments were conducted to determine whether estradiol (E) blocks the effects of T by acting during acquisition or extinction. In the first experiment, gonadectomized male and female rats injected with estradiol dipropionate (EP) and testosterone propionate (TP) during extinction had significantly faster extinction rates than those only injected with TP. Treating gonadectomized rats with TP prior to as well as during extinction did not prevent EP from blocking the effects of T. In Experiment 2, E was equally effective in preventing T from prolonging extinction when it was implanted in gonadectomized males during acquisition, extinction, or both acquisition and extinction. Thus, E does not have to be present concurrently with T during extinction to be effective. This suggests that E does not act on a T-related mechanism but rather acts independently of T.

Efficacy of local graft irradiation in preventing cadaveric renal transplant rejection: a prospective randomized trial.

Successful renal transplantation depends on the modification of the normal immunologic response. The earliest attempts at such modification involved the use of ionizing irradiation. The significant morbidity and mortality of total body irradiation led to its abandonment in favor of the safer technique of local graft irradiation. While still commonly used, the efficacy of this technique has never been evaluated in a prospective, randomized fashion. The present study is a prospective, randomized, double blinded evaluation of the efficacy of the addition of local graft irradiation to our immunosuppressive protocol. One hundred consecutive cadaveric renal transplants were randomized to receive either conventional immunosuppression alone or conventional immunosuppression plus local graft irradiation delivered in doses of 150 rads on the 1st, 3rd and 5th post-operative days. Patients were followed for a period of 2 years. No significant difference was demonstrated between the groups among any measured parameter. In conclusion, the addition of local graft irradiation in doses of 150 rads on d 1, 3, and 5 does not appear to offer any advantage over standard immunosuppressive therapy and its use cannot be recommended.