Adding Granular Activated Carbon and Zerovalent Iron to the High-Solid Anaerobic Digestion System of the Organic Fraction of Municipal Solid Waste: Anaerobic Digestion Performance and Microbial Community Analysis.

Anaerobic digestion (AD) performance and microbial dynamics were investigated in a high-solid anaerobic digestion (HSAD) system of the organic fraction of municipal solid waste (OFMSW). 1, 5, 10, and 15% (w/w, dry weight of the OFMSW) of granular activated carbon (GAC) and zerovalent iron (ZVI) were added to the HSAD system. The results showed that adding ZVI and GAC can improve the methane yield of the OFMSW. Notably, R-(GAC + ZVI) exhibited the highest cumulative methane yield of 343.0 mL/gVS, which was 57.1% higher than that of the R-control. At the genus level, the dominant bacteria included norank_f__norank_o__MBA03, norank_f__norank_o__norank_c__norank_p__Firmicutes, Fastidiosipila, norank_f__Rikenellaceae, and Sphaerochaeta, while Methanoculleus, Methanobacterium, and Methanosarcina were the dominant archaea. The highest relative abundance of norank_f__norank_o__norank_c__norank_p__Firmicutes was 30.8% for the R-(GAC + ZVI), which was 71.4% higher than that of the R-control. The relative abundance of Methanoculleus and Methanobacterium for the R-(GAC + ZVI) and the R-control group accounted for 79.0 and 90.8% of the total archaeal abundance, respectively. Additionally, the relative abundance of Methanosarcina was 10.6% for R-(GAC + ZVI), which was higher than that of the R-control (1.1%). After the addition of GAC and ZVI, the electron transfer capacity of the HSAD system was enhanced, resulting in promoted methane production. Thus, the simultaneous addition of GAC and ZVI to the HSAD system can be an effective strategy to promote the cumulative methane yield of the OFMSW.

Substance bioconversion, hydrolases activity, and metagenomic analysis to unravel the enhanced biomethanation of corn stover with urea-hydrothermal pretreatment.

Corn stover (CS) is a promising feedstock for producing biomethane, that can replace diminishing fossil fuels. However, the recalcitrant structure of CS resulted in low degradability in anaerobic digestion (AD). Numerous studies investigated the pretreatment of CS before AD, but the insight mechanism of biomethanation enhancement is not fully revealed. Therefore, this study advanced low-temperature urea-hydrothermal pretreatment of CS, and the biomethane production, substance bioconversion, hydrolase activity, and metagenomic analysis were conducted to unravel the intrinsic mechanisms of pretreatment for the enhanced biomethanation. The results showed that the pretreatment improved 11.5% of the specific surface area of CS, providing 111.5% higher total volatile fatty acids and 19.9% higher reducing sugars than the control, potentially enriching more anaerobic microorganisms. As a result, the pretreated CS achieved 19.1% higher biomethane yield, 9.1% higher volatile solid removal rate, and 3 days shorter digestion time. The mass balance and microbial community succession analysis indicated that the pretreatment reinforced the biomethane conversion from carbohydrate, which was attributed to the rapid enrichment of hydrolytic acidification bacteria (g__unclassified_o__Bacteroidales) (33.2%) and mixotrophic archaea (Methanosarcina) (72.3%). Meanwhile, the activity of cellulase and xylanase was enhanced up to 23.7% and 66.7%. Metagenomic analysis revealed that the combined pretreatment of CS promoted methanogenesis by enhancing various CAZymes secretion (such as oligosaccharide-degrading enzymes), and functional genes expression of hydrolytic, acidification and acetate-methane pathways at days 1-5. The study indicated that the combined pretreatment could influence microbial composition and function by changing the physicochemical properties of the CS, thereby improving methanogenic performance.

Combined modifications of CaO and liquid fraction of digestate for augmenting volatile fatty acids production from rice straw: Microbial and proteomics insights.

The modification sequence of chemical (CaO) and biological (liquid fraction of digestate, LFD) for augmenting volatile fatty acids (VFAs) production from rice straw was investigated in this study. The coupling order of the modifiers influenced acidification performance, and simultaneous modification (CaO-LFD) was superior to other modes. The highest VFAs production was obtained in CaO-LFD, 51% higher than that in the LFD-first additional modification. The CaO-LFD demonstrated the highest selectivity of acetate production, accounting for 79% of the total VFAs. In addition, CaO-LFD modification changed the direction of the domestication of fermentative bacteria and increased populations of the key anaerobes (Atopostipes sp.) responsible for acidification. The synergistic effect of CaO and LFD was revealed, namely, the effective function of CaO in degrading recalcitrant rice straw, the promotion of transport/metabolism of carbohydrates and acetogenesis by LFD.

Effects of organic loading rate and pretreatments on digestion performance of corn stover and chicken manure in completely stirred tank reactor (CSTR).

The performance, system stability, and microbial community response in anaerobic co-digestion (AcoD) of corn stover (CS) and chicken manure (CM) were investigated by running completely stirred tank reactor (CSTR) under controlled organic loading rate (OLR). Prior to anaerobic digestion (AD), potassium hydroxide (KOH) or liquid fraction of digestate (LFD) was applied to pretreat CS, respectively. The results showed that the daily biogas production (DBP) in co-digestion showed a gradual increasing trend with an increase in the OLR from 65 g TS·L-1 to 100 g TS·L-1. The daily methane production per g volatile solids (DMP-VS) in co-digestion increased by 23.0%-27.1%, 18.7%-18.8%, and 17.5%-18.0% at the OLRs of 65, 80, and 100 g TS·L-1, respectively, upon pretreatment with KOH or LFD, as compared to that in co-digestion CSTR without any pretreatment. In addition, all co-digestion CSTRs were operated in stable state. Approximately half of the total carbon in the substrates was recovered in the form of a biogas product, with the carbon mass balance being impacted by the OLR as well as pretreatment. The diversity as well as function of the microbial community varied in response to different OLRs and pretreatment methods. The majority of bacterial genera were strongly correlated with operational parameters. The study indicates that management of OLR and selection of proper pretreatment method could enhance the efficiency and productivity of CS and CM co-digestion in CSTR.

Effect of wheat straw pretreated with liquid fraction of digestate from different substrates on anaerobic digestion performance and microbial community characteristics.

The effects of liquid fraction of digestate (LFD) pretreatment on anaerobic digestion (AD) performance and microbial community characteristics were estimated. Prior to AD, LFD (LFDSM, LFDFW, and LFDWS) collected separately from three continuously stirred tank reactors (CSTRs) using swine manure (SM), food waste (FW), and wheat straw (WS) as the mono-substrate was applied to pretreat WS. The results showed that AD with LFD pretreatment resulted in biomethane yields of 240.2-277.9 mL·gVS-1, a 33.57%-54.54% improvement over the yield of the control, and also produced a time saving of 32.26%-46.77%. The pretreatment parameters were optimized for LFD pretreatment. The enhancement effect was in the order of LFDFW > LFDSM > LFDWS. Simultaneously, the cellulose, hemicellulose and lignin contents in the WS and their characteristics (surface properties, crystallinity index, etc.) varied accordingly. The function of the microbial community was strengthened during the pretreatment stage, but the structure of the microbial community had a clear response to the LFD source substrates. Bacteroidetes was the most dominant phyla and was positively correlated with the hydrolysis rate. Consequently, the LFD from the different substrates used as pretreat agents could improve the AD performance of WS.

Current development and perspectives of anaerobic bioconversion of crop stalks to Biogas: A review.

As one of the most abundant biomass resources, crop stalks are great potential feedstock available for anaerobic digestion (AD) to produce biogas. However, the specific physical properties and complex chemical structures of crop stalks form strong barriers to efficient AD bioconversion. To overcome these problems, many efforts have been made over the past few years. This paper reviewed recent research in the evolving field of anaerobic bioconversion of crop stalks and was focused on three critical aspects affecting AD performance: various pretreatment methods and their effects on the improvement of crop stalk biodegradability, determination of specific AD operation parameters for crop stalks, and development of AD technologies. Finally, recommendations on the future development of crop stalk AD were proposed.

Biomethane Yield, Physicochemical Structures, and Microbial Community Characteristics of Corn Stover Pretreated by Urea Combined with Mild Temperature Hydrotherm.

The corn stover (CS)'s compact structure makes it challenging for microorganisms to use in anaerobic digestion (AD). Therefore, improving CS biodegradability has become a key focus in AD studies. Methods are being targeted at the pretreatment of CS, combining advanced urea with mild temperature hydrotherm pretreatment to study its effect on promoting the AD process of CS. The biomethane yield, physicochemical structure, and microbial community characteristics were investigated. CS samples were assigned into groups differed by a range of pretreatment times (from 24 to 96 h) and set at a temperature of 50 °C with a 2% urea addition. Results revealed that the 72-h group obtained the highest biomethane yield of 205 mL/g VS-1, volatile solid (VS) and total solid (TS) removal rates of 69.3% and 47.7%, which were 36.7%, 25.3% and 27.5% higher than those of untreated one, respectively. After conducting several analyses, results confirmed the pretreatment as a method for altering CS microstructures benefits biomethane production. The most resounding differences between pretreated and untreated groups were observed within a microbial community, an integral factor for improved AD performance. This study serves to confirm that this specific pretreatment is an effective method for enhancing biomethane production in CS.

Oyster shells improve anaerobic dark fermentation performances of food waste: Hydrogen production, acidification performances, and microbial community characteristics.

Anaerobic dark fermentation (DF) performances of food waste (FW) were investigated using oyster shells. The different amount oyster shells(6%-12%(w/w)) were added to the DF system of FW. The result showed that the H2 production rate and cumulative H2 production improved after addition oyster shells. The highest H2 production rate and cumulative H2 production of 8% oyster shells addition group were 8.4 mL/(gVS·h) and 88.2 mL/gVS, which were 11.7%-30.6% and 17.4%-52.9% higher than those of the other test groups. TVFAs production, especially acetic and butyric acids improved after addition oyster shells. The highest TVFAs production was 19291.4 mg/L for 8% oyster shells added group, which was 90.24% higher than that of the unadded group. For 8% oyster shells added group, Lactobacillales, Gallicola, and Bacteroides were the dominant species at genus levels. Thus, the addition of an appropriate amount oyster shells could improve H2 production rate, cumulative H2 production, promote buffering capacity, enhance TVFAs production.

Performance and microbial community dynamics in anaerobic co-digestion of chicken manure and corn stover with different modification methods and trace element supplementation strategy.

The performance and microbial community dynamics in anaerobic co-digestion (ACoD) of chicken manure and corn stover with different modification methods and trace element supplementation strategy were investigated in this study. KOH and liquid fraction of digestate (LFD) were applied for modification; Fe, Co, Mn, Mo, and Ni were used for supplement. Results showed that the selected trace element was insufficient in the partial or whole digestion process. When trace element supplement was combined with KOH or LFD modifications, the ACoD obtained biomethane yields of 245.3-258.0 and 254.0-261.8 mLN·gVS-1, 26.0%-32.5% and 30.5%-34.5% more than that of the control, respectively. Microbial community analyses indicated that the composition and diversity of archaea and bacteria varied at genus level. Main pathways involved in ACoD were affected accordingly, which in turn affected co-digestion performance. This study demonstrated that the combining modification and trace element supplement could improve the digestion performance and achieve higher biomethane yield.

Anaerobic bioconversion efficiency of rice straw in continuously stirred tank reactor systems applying longer hydraulic retention time and higher load: One-stage vs. Two-stage.

This study investigated the anaerobic bioconversion efficiency of rice straw in continuously stirred tank reactor (CSTR) applying longer hydraulic retention time (HRT) and higher load. Two HRT distributions and two loads were studied and compared for one-stage and two-stage CSTR systems. The results indicated that the two system with longer HRT (60d) and higher load (160g TS·L-1) obtained 11.06% and 14.28%, 15.24% and 19.38%, more biogas and methane productions than those of one-stage system with HRT (50d) and load (140g TS·L-1), respectively, while maintained stable operation at higher loads. It was also found that the microbial richness, diversity, and bacterial and archaeal community compositions showed some differences between two systems with different HRTs and loads, which was thought to be one of reasons leading to the differences in bioconversion efficiencies. The study indicated that two-stage system applying longer HRT and higher load could be one of the effective methods for more bioenergy recovery from rice straw.

Enhanced hydrolysis and acidification strategy for efficient co-digestion of pretreated corn stover with chicken manure: Digestion performance and microbial community structure.

The effect of enhanced hydrolysis and acidification (EHA) strategy on co-digestion performance of pretreated corn stover (CS) with chicken manure (CM) was investigated. The EHA process was applied to the CS pretreated with KOH and liquid fraction of digestate (LFD), prior to anaerobic digestion. The results showed that the efficiencies of hydrolysis and acidification for the pretreated CS group were significantly higher than the CS group. The maximum cumulative biomethane yield of 240.5 mL·gVS-1 and 242.0 mL·gVS-1 were obtained for the KOH CS group and LFD CS group during the EHA process at 1 day, showing 26.6% and 27.4% improvement over that of the control, respectively. T90 was shortened by 38.2%-44.1% and 17.7%-38.2%, correspondingly. The synergistic effects and hydrolysis kinetics were also enhanced by the EHA process. The communities of bacteria (Firmicutes, Proteobacteria, and Bacteroidetes) and archaea (Methanosaeta, Methanobacterium, and Methanosarcina) were enriched by the EHA process, and their interactions contributed to the improved digestion performance. Therefore, the EHA process was recommended for efficient biomethane conversion in co-digestion of CS and CM.

Effect of ultrasonic application during KOH pretreatment and anaerobic process on digestion performance of wheat straw.

The digester performance was enhanced by ultrasonic application during pretreatment and the anaerobic digestion (AD) process. Two setups (with and without ultrasonic application) were applied during pretreatment and AD, to untreated and potassium hydroxide (KOH) pretreated wheat straw. The results confirmed that the ultrasonic application enhanced the hydrolysis process during pretreatment. The highest total volatile fatty acid (TVFA) (3012 ± 18 mg L-1) production and overall lignin, hemicellulose, and cellulose (LHC) reductions (22.4 ± 0.5%) were obtained from ultrasonic assisted KOH pretreated (KOHU) samples, after 36 hours of pretreatment. Similarly, the SEM analysis showed obvious disruption in the outer structure of KOHU samples. However, the ultrasonic application during AD showed significant improvement in biodegradation rate, biogas and biomethane production. Obvious reduction in sonication time (76%) along with enhanced biogas (570 ± 9 mL gm-1 VS) and biomethane (306 ± 12 mL gm-1 VS) production were observed from KOH pretreated digesters, when ultrasonication was applied during AD. Moreover, the biodegradation rate reached up to 76% along with highest total solid (TS) and volatile solid (VS) reductions from digesters with single KOH pretreatment and ultrasonic influence during AD. Finally, the digester effluent ranged in between the stable values, confirming the completion of the AD process. These results suggested that ultrasonic application was more effective when applied during AD due to the higher liquid to solid ratio. The reduction in energy input can be beneficial for commercial applications and to recognize the better stage for ultrasonic application for enhanced biomethane yield.

Mass conversion pathway during anaerobic digestion of wheat straw.

A material flow analysis (MFA) method was employed to investigate elemental flow direction during the anaerobic digestion (AD) of wheat straw (WS) pretreated with potassium hydroxide. A lab-scale batch AD experiment conducted at 35 ± 1 °C was investigated to realize carbon conversion in biogas, liquid and solid digestates. The results showed that the highest growth rate of carbon conversion in biogas was observed from the fourth day to the twenty-fourth day, which accounted for 70.64%. The cumulative biogas production of WS was 531 mL g-1 VS, along with a high volatile solids degradation rate (55.0%). The MFA results indicated that the flow mass fractions of carbon in biogas, liquid and solid digestates were 49.96%, 5.61% and 44.43%, respectively. The flow mass fraction of nitrogen in liquid and solid digestates was 45.65% and 54.35%, respectively. This study can provide a theoretical basis for elemental flow in each product from biogas projects.

Effects of different potassium and nitrogen pretreatment strategies on anaerobic digestion performance of rice straw.

The effects of different potassium and nitrogen pretreatment strategies on the anaerobic digestion (AD) performance of rice straw (RS) were investigated. KOH, NH3·H2O and KOH + NH3·H2O combined pretreatments were applied. The results showed that KOH + NH3·H2O combined pretreatment achieved the highest biomethane production and TS (TS: total solid) removal rate of 274 mL g VS-1 and 43.9%, which were 6.2-75.8% and 4.3-29.5% higher than that of single alkali pretreatments and untreated RS, respectively. The NH3·H2O groups improved the process stability, which maintained the NH3-N concentration in the range of 265-580 mg L-1. It was also found that Bacteroidetes and Firmicutes were the dominant bacterial at phyla level, and the populations of acetate methanogen (Methanosarcina and Methanosaeta) were enriched in the AD system by KOH + NH3·H2O pretreatment. Furthermore, the cost of pretreatment agents can be recovered by the increased digestate nutritional value due to the K and N remaining in the digestate after AD. The results indicated that the KOH + NH3·H2O combined pretreatment might be a promising method for efficient AD of straw in future industrial applications.

Multi-perspective analyses of rice straw modification by Pleurotus ostreatus and effects on biomethane production.

Multi-perspective analyses were carried out to investigate the effect of rice straw modification for 45 days by P. ostreatus on biomethane of production. The results showed that rice straw modified for 25 days achieved the highest biomethane yield of 269 mL·g-1 VS, which was a 26.9% improvement compared with non-modified rice straw. The multi-perspective analyses demonstrated that the improvement resulted from fungal enzymatic reactions, which led to changes in the physicochemical properties of rice straw. The porosity, surface area, acetyl group abundance, degree of polymerization, and lignin degradation selectivity of rice straw modified for 25 days were optimal for enzyme adsorption. Compared with non-modified rice straw, the adsorption of cellulase and xylanase on rice straw modified for 25 days was increased by 18.8% and 58.1%, respectively, which facilitated biomethane production. The study indicated that P. ostreatus is effective for improving biomethane production from rice straw.

Anaerobic co-digestion of cattle manure and liquid fraction of digestate (LFD) pretreated corn stover: Pretreatment process optimization and evolution of microbial community structure.

Liquid fraction of digestate (LFD) was used to pretreat corn stover to enhance the biomethane production of anaerobic co-digestion (AcoD) with cattle manure. The effects of LFD concentration and water content (WC) for pretreatment on co-digestion performance and microbial community structure were investigated in a batch system. Results showed that the cumulative biomethane yield (CBY) for co-digestion was improved by 16.85%-41.78% compared with the control. The highest biomethane yield of 238.25 mL g VS-1 was obtained at 85% WC for pretreatment and a 5 M LFD concentration, and this yield was 41.78% higher than that in the control. The LFD pretreatment enriched the dominant bacterial phyla (Firmicutes and Bacteroidetes), but had little influence on the prevalent archaeal genus (Euryarchaeota). This study demonstrated that LFD pretreatment can greatly enhance the biomethane yield of co-digestion of corn stover and cattle manure under optimal parameters.

Effect of low severity hydrothermal pretreatment on anaerobic digestion performance of corn stover.

The anaerobic digestion (AD) performance of corn stover was investigated under low hydrothermal pretreatment (HP) severity. Pretreated corn stover, with and without ammonia was examined at the three temperatures (50, 70, and 90 °C) coupled with different pretreatment time (24-72 h). The results showed that the pH value decreased with increasing pretreatment time for the same temperature. The highest volatile fatty acids (VFAs) concentration (4616.23 mg/L) was achieved at a HP severity of 4.56. The highest removal rate of cellulose, hemicellulose, and lignin were 19.62%, 25.53%, and 32.91% for HP with ammonia (HPA) severities of 3.34, 3.34, and HP severity of 4.35, respectively. The maximum methane yield of corn stover was 148.19 mL/g∙VS under 50 °C held for 24 h (HPA severity of 2.59), which was 31.35% higher than that of the untreated corn stover. The research results suggest that properly pretreatment severity can play an important role in promoting methane production.

Evaluation of system stability and anaerobic conversion performance for corn stover using combined pretreatment.

A novel pretreatment method combining freezing-thawing and ammonia was developed, and the system stability and anaerobic conversion performance of corn stover (CS) were investigated. The CS was pretreated by water freezing-thawing (water-FT) and ammonia freezing-thawing (ammonia-FT), and then pretreated and untreated CS were anaerobically digested in three continuously stirred tank biodigesters (CSTBRs) at three organic loading rate (OLR) of 1.6, 1.8 and 2.0 gVS L-1 d-1. The analyses showed that pH value and volatile fatty acids (VFAs) concentration of three systems were in reasonable ranges of 6.8-7.5 and 160-330 mg L-1, respectively. Total ammonia nitrogen (TAN) in R3 system ranged from 600 mg L-1 to 1300 mg L-1, which was conducive to maintain system stability. The removal amount of soluble chemical oxygen demand (SCOD) of CS by ammonia-FT pretreatment was 17.3-20.5% higher than that of water-FT pretreatment. The results also showed that CS pretreated by ammonia-FT achieved 27.8-32.4% and 13.9-16.1% more methane yield (250-267 mL gvs-1) than those of untreated and water-FT pretreatment, respectively. Correspondingly, ammonia-FT pretreated CS in R3 obtained the highest conversion rates of 47.7%, 56.9%, 42.7%, and 48.8% for TS, VS, cellulose, and hemicellulose, respectively, and the lowest cost was of 0.0336 USD m3 CH4-1 and the highest energy recovery of 2428.1 kWh kgvs-1. The study demonstrated that ammonia-FT pretreatment is one of efficient and cost effective method for stable CS bioconversion.

Continuous dynamics in anaerobic reactor during bioconversion of rice straw: Rate of substance utilization, biomethane production and changes in microbial community structure.

Anaerobic digestion (AD) technology is one of the best eco-friendly waste-to-energy processes. Hence the trend of biogas production shows considerable ranges of fluctuations in entire digestion period. This study analyzes the daily substance conversion dynamics, biomethane production and changes in microbial community structure. The results show that in the first peak of biogas production during 1st up to 4th days of the digestion period, CO2 was the dominant component. However, the daily methane production (DMP) reveal accelerated increment starting from day 8 up to the peak point on day 13 (462.11 mL/d). The concentration of acetic acid covered 21.80% up to 62.00% of the total VFAs in the first 10 days of digestion period. On the other hand, the accumulation of propionic acid is in the range of 1735.70 mg/L- 2893.12 mg/L in between day 5-15, which is beyond the inhibition level (1000 mg/L) but the system didn't stop biogas production. The distribution of bacterial family such as Clostridiaceae_1, Prevotellaceae, Enterobacteriaceae and Peptostreptococcaceae are the dominant group at early stage as compared to composition in remaining stages except Enterobacteriaceae which have marginally high abundance in lowest biogas production point. The archaeal genus Methanosaeta is dominant among the samples collected at early stages (65.66%-77.22%). However, the Methanobacterium is predominant (34.88%-59.40%) in samples obtained at late stages of AD period. On the other hand, the distribution of Methanosarcina is comparable in the first three samples (S1(16.60%), S2(22.21%) and S3(20.38%)) than the stable stage (S4 (6.7%)). This study demonstrates the detailed conditions at fluctuating and constant biogas production periods, which would benefit future researchers working in similar area.

A novel strategy for efficient anaerobic co-digestion based on the pretreatment of corn stover with fresh vinegar residue.

A novel method was advanced for efficient anaerobic co-digestion by using fresh vinegar residue (FVR) as acidifier for pretreating corn stover (CS). FVR acted as one substrate as well as an acidifier by the acids contained in FVR. It was found that the organic acids in FVR could efficiently enhance the hydrolysis of lignocellulose in CS. The biomethane production from co-digestion of FVR and CS pretreated reached 140.48 L/kg VS, which was 35.7% higher than that of unpretreated mixture substrates. The highest biomethane production was obtained when pretreatment was conducted at 150 °C. The increase of biomethane production was contributed to the improved hydrolysis of CS due to the acidic pretreatment. Pretreatment and co-digestion could improve the asynchronism and generate synergistic effect. The study provides one novel method for efficient biomethane conversion from FVR and CS.