ABSTRACT
OBJECTIVE: To analyze nucleosides and nucleobases of Pseudostellariae Radix cultivated in different idibplasni resources and to compare the differences. METHODS: QTRAP LC-MS/MS method was applied for the analysis of 13 kinds of nucleosides and nucleobases in Pseudostellariae Radix and the data obtained was analyzed by SPSS 16. 0 software. RESULTS: There were some differences between Pseudostellariae Radix cultivated in different idioplasm resources. The highest amount of nucleosides and nucleobases was ZS2 which came from Zherong in Fujian Province. The total content of nucleosides and nucleobases in the sample from Shibing in Guizhou Province was the lowest. There was little difference between ZS1 (Zherong in Fujian Province) and XC(Xuancheng in Anhui Province). CONCLUSION: This study provides evidence for the influence of eco-environment on the metabolites of Pseudostellariae Radix.
Subject(s)
Caryophyllaceae/chemistry , Nucleosides/analysis , Plant Roots/chemistry , Chromatography, Liquid , Drugs, Chinese Herbal/chemistry , Mass Spectrometry , Tandem Mass SpectrometryABSTRACT
OBJECTIVE: To characterize the different varieties of Pseudostellaria heterophylla during cultivation. METHOD: Using systematic selection in the main productive areas, the techniques of random design, all varieties were observed for 3 years. RESULT: The biological and 425 productive characteristics of P. heterophylla var. macrophylla, P. heterophylla var. Foliolum, and P. heterophylla var. anvense were significantly different (P < 0.01). There were also differences in ecological adaptability, plant characteristics, pollen granule, chromosomes, and isoenzyme of the three cultivars. CONCLUSION: The strain types of P. heterophylla was denominated for the first time. The characteristics and productivity index system of P. heterophylla varieties were determined.
