Transcriptome analysis and screening of putative sex-determining genes in the invasive pest, Frankliniella occidentalis (Thysanoptera: Thripidae).

The invasive insect pest, Frankliniella occidentalis, is a well-known vector that transmits a variety of ornamental and vegetable viruses. The mechanistic basis of sex determination in F. occidentalis is not well understood, and this hinders our ability to deploy sterile insect technology as an integrated pest management strategy. In this study, six cDNA libraries from female and male adults of F. occidentalis (three biological replicates each) were constructed and transcriptomes were sequenced. A total of 6000 differentially-expressed genes were identified in the two sexes including 2355 up- and 3645 down-regulated genes. A total of 149 sex-related genes were identified based on GO enrichment data and included transformer-2 (tra2), fruitless (fru), male-specific lethal (msl) and sex lethal (sxl); several of these exhibited sex-specific and/or sex-biased expression in F. occidentalis. This study contributes to our understanding of the sex-determined cascade in F. occidentalis and other members of the Thysanoptera.

Identification, expression analysis and functional verification of two genes encoding small heat shock proteins in the western flower thrips, Frankliniella occidentalis (Pergande).

Small heat shock proteins (sHSPs) help prevent the irreversible aggregation of denatured proteins that occurs in response to organismal stress. In this study, we identified two intron-free genes encoding sHSPs from Frankliniella occidentalis; these were designated FoHSP11.6 and FoHSP28.0 and belonged to an atypical and typical sHSP family, respectively. Both FoHSPs were transcribed in all developmental stages of F. occidentalis with the highest expression levels in pupae and adults and greater expression in males than females. Although the FoHSPs had different temperature-induced expression profiles, they were generally induced by both low and high temperatures and reached maximal expression levels after 0.5-1 h of temperature stress. The FoHSPs expression levels in pupae were induced by drought and high humidity, and higher expression levels were correlated with lower survival rates. The thermotolerance of F. occidentalis decreased when theFoHSPs were silenced by RNA interference. Our results show that FoHSP11.6 and FoHSP28.0 are involved in the response to temperature and drought and may also function in growth and development of F. occidentalis.

Differential regulation of antioxidant enzymes in Frankliniella occidentalis (Thysanoptera: Thripidae) exposed to thermal stress.

Frankliniella occidentalis is an invasive insect pest that incites damage to ornamental and agronomic crops on a global scale. In this study, the effects of temperature on gene expression and enzyme activity were studied for superoxide dismutase (SOD), peroxidase (POD), and glutathione-S-transferase (GST) in F. occidentalis. SOD, POD and GST enzyme activity increased significantly at 35-37 °C but declined as the temperature increased to 41 °C. In a time course study at 35 °C, SOD, POD and GST activities were significantly elevated at 0.5, 1 and 2 h in comparison to the control at 26 °C. Expression patterns were evaluated for the three antioxidant genes under high and low temperature stress. In a time course study at -4 °C, SOD, POD and GST expression peaked at 1 h and declined at 2 h of exposure. In contrast, when transcription was monitored at 35 °C, expression was lowest at 1 h and increased at 2 h. The results provide data that will be useful in deciphering the role of antioxidant enzymes in the adaptation of F. occidentalis to climate change.

The Differing Roles of Cognitive Empathy and Affective Empathy in the Relationship Between Trait Anger and Aggressive Behavior: A Chinese College Students Survey.

Empathy is essential for effective social interaction. People often express the belief that empathy is closely related to aggressive behavior, but empirical data has challenged this assumption. However, there is a lack of research that focuses on the role of empathy in the relationship between trait anger and aggressive behavior. The current research focuses on the roles that different components of empathy have performed in the combinations of trait anger-hostile cognition-aggressive behavior link and attempt to identify, with reference to Integrated Model of Emotion Processes and Cognition in Social Information Processing, at which step this may occur. Participants included 663 undergraduate students who completed self-report measures of Trait Anger Scale, Aggressive Behavior Questionnaire, the Interpersonal Reactivity Index, and the Reactive-Proactive Aggression Questionnaire. Results from correlation analysis show that there is no significant correlation between cognitive empathy and aggressive behavior (r = -.06) but do however suggest a significant correlation between affective empathy and aggressive behavior (r = -.19). Results from structural equation modeling reveal that different components of empathy perform different roles in relation to aggressive behavior. The moderated mediating model analysis results show that cognitive empathy played a moderating role in both the direct effect and the first stage of the mediating model of trait anger-hostile cognition-aggression behavior. The results of multiple mediation model analysis demonstrate that affective empathy only played a partial mediating role between hostile cognition and aggressive behavior. This study contributes to understanding of Social Information Processing Models (SIPMs) and provides insight into the relationship between empathy and aggressive behavior.

Cloning of a new HSP70 gene from western flowerthrips, Frankliniella occidentalis, and expression patterns during thermal stress.

Frankliniella occidentalis (Pergande) is an invasive pest that endangers a wide variety of horticultural and agronomic crops. HSP70 is the most important member of the heat shock protein (HSP) family and plays an important role in insect thermal tolerance. In this study, a new gene encoding HSP70 from F. occidentalis, Fohsp706, was selected from the F. occidentalis transcriptome exposed to thermal stress (40 °C) and cloned by RT-PCR and RACE. Further characterization indicated that Fohsp706 localizes to the cytoplasm and does not contain introns. Quantitative real-time reverse transcriptase PCR indicated that Fohsp706 expression was significantly up-regulated by thermal stress; furthermore, there were significant differences in Fohsp706 expression in adults and second instar nymphs after heat stress. Our results indicated that Fohsp706 contributes to thermotolerance in F. occidentalis and provides another example of how this pest adapts to unfavorable environmental conditions.

[Chemical Composition of Alkanes and Organic Acids in Vehicle Exhaust].

The soot of 11 diesel buses and 20 gasoline cars was sampled by direct sampling, and the main organic compounds of the samples were separated and qualitatively analyzed by GC-MS. The results showed that the mass fraction of n-alkanes was 1.31-149.08 µg·g-1 and 15.35-556.03 µg·g-1. The main carbon number of n-alkanes in diesel buses and gasoline cars soot was 20 and 29, respectively, and gasoline cars generated more long chain alkanes with high boiling point. The total mass fraction of pristane and phytane in diesel buses was 15.24 µg·g-1 and 21.79 µg·g-1, respectively, while the two values in gasoline cars were 2.31 µg·g-1 and 2.69 µg·g-1, respectively. The ratios of pristane and phytane were 0.6994 and 0.8587, and the ratios of phytane and octadecane were 0.3565 and 0.7472. The changes of the relative mass of fatty acids were relatively large, the change ranges in buses and cars were 0.01-40.87 µg·g-1 and 3.8-113.7 µg·g-1, respectively, and the organic acid with the highest content in the buses was 3- hydroxybutyric acid.The maximal ratio of hexadecanoic acid and butanedioic acid (C3/C4) was 5.93. The most abundant n-alkanes in gasoline cars soot was C16, and the mass fraction of aromatic acids was 5.05-31.70 µg·g-1 and 0.1-228.38 µg·g-1, respectively.