ABSTRACT
Stem cell therapy is widely employed for the treatment of skin diseases, especially in skin rejuvenation. Exosomes derived from stem cells have been demonstrated to possess anti-photoaging effects; however, the precise components within exosomes that are responsible for this effect remain unknown. Previously, miR-1246 was found to be one of the most abundant nucleic acids in adipose-derived stem cells (ADSCs)-derived exosomes. This study examined whether miR-1246 was the major therapeutic agent employed by ADSCs to protect against UVB-induced photoaging. Lentivirus infection was used to obtain miR-1246-overexpressing ADSCs and exosomes. We then determined the anti-photoaging effects of miR-1246-overexpressing exosomes (OE-EX) on both UVB-irradiated human skin fibroblasts (HSFs) and Kunming mice. The results showed that OE-EX could significantly decrease MMP-1 by inhibiting the MAPK/AP-1 signaling pathway. Meanwhile, OE-EX markedly increased procollagen type I secretion by activating the TGF-ß/Smad pathway. OE-EX also exhibited an anti-inflammatory effect by preventing the UVB-induced degradation of IκB-α and NF-κB overexpression. Animal experiments demonstrated that OE-EX could reduce UVB-induced wrinkle formation, epidermis thickening, and the loss of collagen fibers reduction in Kunming mice. The combined results suggested that miR-1246 is the key component within ADSCs-derived exosomes that protects against UVB-induced skin photoaging.
Subject(s)
Exosomes , MicroRNAs , Skin Aging , Skin Diseases , Mice , Animals , Humans , Transcription Factor AP-1/metabolism , Transforming Growth Factor beta/metabolism , Transforming Growth Factor beta/pharmacology , Exosomes/metabolism , Skin , MicroRNAs/genetics , MicroRNAs/metabolism , MicroRNAs/pharmacology , Skin Diseases/metabolism , Ultraviolet Rays , FibroblastsABSTRACT
In poly[[µ3-2,2'-(disulfanediyl)dibenzoato-κ(5)O:O,O':O'',O'''](1,10-phenanthroline-κ(2)N,N')cadmium(II)], [Cd(C14H8O4S2)(C12H8N2)]n, the asymmetric unit contains one Cd(II) cation, one 2,2'-(disulfanediyl)dibenzoate anion (denoted dtdb(2-)) and one 1,10-phenanthroline ligand (denoted phen). Each Cd(II) centre is seven-coordinated by five O atoms of bridging/chelating carboxylate groups from three dtdb(2-) ligands and by two N atoms from one phen ligand, forming a distorted pentagonal-bipyramidal geometry. The Cd(II) cations are bridged by dtdb(2-) anions to give a two-dimensional (4,4) layer. The layers are stacked to generate a three-dimensional supramolecular architecture via a combination of aromatic C-H···π and π-π interactions. The thermogravimetric and luminescence properties of this compound were also investigated.
ABSTRACT
The title compound, [Fe(C(5)H(5))(C(15)H(14)NO(2))], is a monoclinic polymorph of the previously reported triclinic form [Shi et al. (2006 â¶). Acta Cryst. C62, m407-m410]. The polymorphs feature the same strong intra-molecular N-Hâ¯O=C hydrogen bonds, but show different packing modes. The mol-ecules in the monoclinic form associate into double chains via O-Hâ¯O=C and (Cp)C-Hâ¯O-H inter-actions.
ABSTRACT
In the title compound, [Fe(C(5)H(5))(C(17)H(16)NO(4))], the O=C-C=C-N mean plane is twisted with respect to the mean planes of the benzene and substituted cyclo-penta-dienyl rings by 44.2â (2) and 13.8â (3)°, respectively. Furthermore, the O=C-C=C-N mean plane and the O=C-O(ester) plane make a dihedral angle of 55.5â (6)°. Consistent with this large dihedral angle, the linking C-C bond [1.507â (6)â Å] does not show any (delocalized) double-bond character.
ABSTRACT
The title compound, [Fe(4)(C(6)H(6)NS)(CH(3)S)S(CO)(11)], com-prises two butterfly-shaped sub-cluster cores, Fe(2)S(2)N and Fe(2)S(2), joined together by a spiro-type µ(4)-S atom. The (pyridin-2-yl)methane-thiol-ate ligand is attached to the Fe(2)(CO)(5) unit in a µ-κN:κ(2)S mode, and the methane-thiol-ate ligand is coordinated to the Fe(2)(CO)(6) unit in a µ-κ(2)S fashion.
ABSTRACT
The fused bis-butterfly-shaped title compound, [Fe(4)(CS(4))(CO)(12)], possesses an orthothio-carbonate (CS(4) (4-)) ligand that acts as a bridge between two Fe(2)(CO)(6) units. A short intra-molecular Sâ¯S contact [2.6984â (8) and 2.6977â (8)â Å] occurs in each S(2)Fe(2)(CO)(6) fragment.
ABSTRACT
In the crystal structure of the title compound, [Co(H(2)O)(6)][Cu(C(12)H(11)N(2)O(5))](2)·6H(2)O, the Co(II) atom is located on an inversion center and coordinated by six water mol-ecules in a slightly distorted octa-hedral geometry. The Cu(II) atom is chelated by the Schiff base ligand in a distorted CuN(2)O(2) square-planar geometry. An extensive O-Hâ¯O hydrogen-bonding network is present in the crystal structure.
