[Effects of oral propranolol on urine bFGF, MMP-2, MMP-9 expression in children with proliferative infantile hemangioma].

PURPOSE: To investigate the effect of propranolol on urine bFGF, MMP-2, MMP-9 expression of children with proliferative infantile hemangioma(IH), so as to clarify the mechanism of propranolol in treating IH. METHODS: From June 2018 to June 2019, thirty-four children with proliferative IH were treated with oral propranolol. In addition, twenty-one normal children (age <12 months) were chosen as the control group. 10 mL of sterile morning urine were collected before and 2 months after oral administration of propranolol in infants with IH. All blood samples were placed in ordinary disinfection test tubes, centrifuged at 1 000 r/min for 10 min, the supernatant of urine was collected and stored separately. The urine samples of normal control group were processed in the same way. The expression levels of bFGF in the urine of children with proliferative IH before and 2 months after oral administration of propranolol and in the normal control group were detected by enzyme-linked immunosorbent assay (ELISA). The expression levels of MMP-2 and MMP-9 in the urine of children with proliferative IH before and 2 months after treatment and in the control group were detected by gelatin zymography. SPSS 22.0 software package was used to analyze the data. RESULTS: Two months after oral propranolol treatment, the concentration of bFGF in urine was significantly lower than that before treatment (P<0.01), but still significantly higher than that in the control group (P<0.05). The expression levels of MMP-2 and MMP-9 were significantly lower than those before treatment(P<0.01), but still higher than those in the control group (P<0.05). CONCLUSIONS: One of the mechanisms of propranolol in the treatment of children with proliferative IH may be through inhibiting the expression levels of bFGF, MMP-2 and MMP-9, and then inhibiting the proliferation and angiogenesis of vascular endothelial cells in IH, so as to achieve the effect of treating hemangioma. The detection of the expression levels of bFGF, MMP-2 and MMP-9 in urine can be used as the index for oral propranolol treatment of children with proliferative IH.

Synthesis and characterization of carbon nanofibers by catalytic chemical vapor deposition using non-ferromagnetic metal complexes.

Carbon nanofibers (CNFs) have wide applications in energy storage devices, electrically conducting composites, selective adsorbents, and catalyst supports. Catalytic chemical vapor deposition was carried out in this work to synthesize CNFs at mild temperatures of 700 and 800 degrees C. Non-ferromagnetic metal complexes of La, Nb, and Ti, spread on porous NaX-type zeolite support, were tested as new catalyst. CNFs ranging from 30 to 200 nm in diameter were obtained. Images of transmission electron microscopy showed encapsulated transition-metal nanoparticles by CNFs. X-ray diffraction patterns revealed the crystalline structures of La (FCC), Nb (BCC), and Ti (HCP) formed over zeolite. Magnetic hysteresis loops showed superconductivity from the CNF-encapsulated Nb at 2 K. Raman spectra showed that all the samples possessed graphitic and amorphous carbon structures. Based on the SEM images and Raman spectra, the three metals all catalyzed the synthesis of CNFs.

[The efficacy of treatment of oral and maxillofacial venous malformations by pingyangmycin injection with different concentrations].

PURPOSE: To evaluate the clinical effect of using different concentrations of pingyangmycin to treat venous malformations in oral and maxillofacial region. METHODS: One hundred and ninety-four patients with venous malformations in oral and maxillofacial region were included and treated by pingyangmycin injection with different concentrations. For superficial cutaneous venous malformations, the concentration of pingyangmycin was 8mg/mL while 8mg/5mL for venous malformations limited to oral mucosa or submucosa. RESULTS: The efficient of cutaneous venous malformations was 94.9%,while 100% for oral mucosal and submucosa. CONCLUSIONS: Using different concentrations of pingyangmycin for venous malformations in different sites can get good results and reduce unnecessary complications.

Propranolol given orally for proliferating infantile haemangiomas: analysis of efficacy and serological changes in vascular endothelial growth factor and endothelial nitric oxide synthase in 35 patients.

We investigated the clinical efficacy of propranolol in treating proliferating infantile haemangiomas, and the serological changes in vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) during treatment. Propranolol was given orally to 35 infants with proliferating haemangiomas in a dose of 1.0-1.5mg/kg/day. Hemisphere measurements, the 4-score method, and feedback from parents were used to evaluate the clinical curative effects. An ELISA was used to measure the serum concentrations of VEGF and eNOS before, and 1 and 2 months after, treatment. The response was excellent in 6 patients, good in 16, moderate in 10, and poor in 3. The peripheral serum concentrations of VEGF and eNOS 2 months after treatment were significantly lower than those before treatment (p<0.01). We conclude that propranolol can be safely and effectively used to treat proliferating infantile haemangiomas. It can reduce the peripheral serum concentrations of VEGF and eNOS in affected children.

[Electrochemical therapy combined with pingyangmycin injection in the management of high-return flow venous malformation].

OBJECTIVE: To investigate the clinical effect of electrochemical therapy (ECT) combined with pingyangmycin (PYM) injection for the treatment of high-return flow venous malformation. METHODS: A total of 68 cases of the high-return flow venous malformation were retrospectively analyzed, in which 32 cases received simplely ECT,and 36 cases received ECT combined with PYM injection. The patients were followed up from 6 to 30 months. RESULTS: The effectiveness of ECT was 78% (25/32), and of ECT combined with PYM injection was 97% (35/36). The effectiveness of ECT combined with PYM injection was higher than that of ECT (P < 0.05). No serious adverse effects were encountered. CONCLUSIONS: ECT combined with PYM injection is an effective method for the treatment of high-return flow venous malformation.

Supernatant decanting on a centrifugal platform.

This study presents a novel approach to decant supernatant on a centrifugal platform. By manipulating the centrifugally induced pressure and the elastic deformation of the plastic lids in the decanting chamber, fixed amounts of the supernatant can be decanted into the detection chamber at lower rotational speeds. The experimental results showed that decanted volume is affected by the volume of deformation and the operating parameters. Factors that influence the decanting ratio are also discussed. This approach has the advantages of simple design and low manufacturing cost; further, it has no need of surface modification. It has been applied to on-disk separation of plasma from whole blood, and the results showed good stability and repeatability.

[Expression and correlation of mast cell, Clusterin/apoJ and transforming growth factor-beta in the different stages of human dermal hemangioma].

OBJECTIVE: To investigate the expression and correlation of mast cell, Clusterin/apoJ and transforming growth factor-beta (TGF-beta) in the different stages of human dermal hemangioma. METHODS: Immunohistochemical stain technique (SABC) and toluidine blue (TB) stain technique were respectively used to detect the expression level of Clusterin/apoJ and TGF-beta and the number of mast cells in the different stages of human dermal hemangioma. RESULTS: There was remarkable statistical difference between the advanced stage of proliferative hemangioma and the other stages of proliferative hemangioma in the number of mast cell(P<0.01). There was also remarkable statistical difference between the early stage of involutional hemangioma and the other stages of involutional hemangioma in the number of mast cell (P<0.01). The expression of Clusterin/apoJ and TGF-beta in the advanced stage of proliferative hemangioma was significantly higher than the other stages in proliferative hemangioma (P<0.01). The expression of Clusterin/apoJ and TGF-beta in the early stage of involutional hemangioma was significantly higher than the other stages in involutional hemangioma (P<0.01). There was a significantly positive correlation between Clusterin/apoJ and TGF-beta in the different stages of human dermal hemangioma (P<0.01). The expression level of Clusterin/apoJ and TGF-beta was positively correlated with the number of mast cell in the different stages of human dermal hemangioma (P<0.01). CONCLUSION: Mast cell may play a promotive role of apoptosis during the spontaneous regulate the expression of Clusterin/apoJ and promote the spontaneous involution of human dermal hemangioma.