Enhanced rock weathering increased soil phosphorus availability and altered root phosphorus-acquisition strategies.

Enhanced rock weathering (ERW) has been proposed as a measure to enhance the carbon (C)-sequestration potential and fertility of soils. The effects of this practice on the soil phosphorus (P) pools and the general mechanisms affecting microbial P cycling, as well as plant P uptake are not well understood. Here, the impact of ERW on soil P availability and microbial P cycling functional groups and root P-acquisition traits were explored through a 2-year wollastonite field addition experiment in a tropical rubber plantation. The results show that ERW significantly increased soil microbial carbon-use efficiency and total P concentrations and indirectly increased soil P availability by enhancing organic P mobilization and mineralization of rhizosheath carboxylates and phosphatase, respectively. Also, ERW stimulated the activities of P-solubilizing (gcd, ppa and ppx) and mineralizing enzymes (phoADN and phnAPHLFXIM), thus contributing to the inorganic P solubilization and organic P mineralization. Accompanying the increase in soil P availability, the P-acquisition strategy of the rubber fine roots changed from do-it-yourself acquisition by roots to dependence on mycorrhizal collaboration and the release of root exudates. In addition, the direct effects of ERW on root P-acquisition traits (such as root diameter, specific root length, and mycorrhizal colonization rate) may also be related to changes in the pattern of belowground carbon investments in plants. Our study provides a new insight that ERW increases carbon-sequestration potential and P availability in tropical forests and profoundly affects belowground plant resource-use strategies.

A daily gap-free normalized difference vegetation index dataset from 1981 to 2023 in China.

Long-term, daily, and gap-free Normalized Difference Vegetation Index (NDVI) is of great significance for a better Earth system observation. However, gaps and contamination are quite severe in current daily NDVI datasets. This study developed a daily 0.05° gap-free NDVI dataset from 1981-2023 in China by combining valid data identification and spatiotemporal sequence gap-filling techniques based on the National Oceanic and Atmospheric Administration daily NDVI dataset. The generated NDVI in more than 99.91% of the study area showed an absolute percent bias (|PB|) smaller than 1% compared with the original valid data, with an overall R2 and root mean square error (RMSE) of 0.79 and 0.05, respectively. PB and RMSE between our dataset and the MODIS daily gap-filled NDVI dataset (MCD19A3CMG) during 2000 to 2023 are 7.54% and 0.1, respectively. PB between our dataset and three monthly NDVI datasets (i.e., GIMMS3g, MODIS MOD13C2, and SPOT/PROBA) are only -5.79%, 4.82%, and 2.66%, respectively. To the best of our knowledge, this is the first long-term daily gap-free NDVI in China by far.

Age-Related Conservation in Plant-Soil Feedback Accompanied by Ectomycorrhizal Domination in Temperate Forests in Northeast China.

This study investigates the effects of forest aging on ectomycorrhizal (EcM) fungal community and foraging behavior and their interactions with plant-soil attributes. We explored EcM fungal communities and hyphal exploration types via rDNA sequencing and investigated their associations with plant-soil traits by comparing younger (~120 years) and older (~250 years) temperate forest stands in Northeast China. The results revealed increases in the EcM fungal richness and abundance with forest aging, paralleled by plant-soil feedback shifting from explorative to conservative nutrient use strategies. In the younger stands, Tomentella species were prevalent and showed positive correlations with nutrient availability in both the soil and leaves, alongside rapid increases in woody productivity. However, the older stands were marked by the dominance of the genera Inocybe, Hymenogaster, and Otidea which were significantly and positively correlated with soil nutrient contents and plant structural attributes such as the community-weighted mean height and standing biomass. Notably, the ratios of longer-to-shorter distance EcM fungal exploration types tended to decrease along with forest aging. Our findings underscore the integral role of EcM fungi in the aging processes of temperate forests, highlighting the EcM symbiont-mediated mechanisms adapting to nutrient scarcity and promoting sustainability in plant-soil consortia.

Relationships between Phyllosphere Bacterial Communities and Leaf Functional Traits in a Temperate Forest.

As a vital component of biodiversity, phyllosphere bacteria in forest canopy play a critical role in maintaining plant health and influencing the global biogeochemical cycle. There is limited research on the community structure of phyllosphere bacteria in natural forests, which creates a gap in our understanding of whether and/or how phyllosphere bacteria are connected to leaf traits of their host. In this study, we investigated the bacterial diversity and composition of the canopy leaves of six dominant tree species in deciduous broad-leaved forests in northeastern China, using high-throughput sequencing. We then compare the differences in phyllosphere bacterial community structure and functional genes of dominant tree species. Fourteen key leaf functional traits of their host trees were also measured according to standard protocols to investigate the relationships between bacterial community composition and leaf functional traits. Our result suggested that tree species with closer evolutionary distances had similar phyllosphere microbial alpha diversity. The dominant phyla of phyllosphere bacteria were Proteobacteria, Actinobacteria, and Firmicutes. For these six tree species, the functional genes of phyllosphere bacteria were mainly involved in amino acid metabolism and carbohydrate metabolism processes. The redundancy and envfit analysis results showed that the functional traits relating to plant nutrient acquisition and resistance to diseases and pests (such as leaf area, isotope carbon content, and copper content) were the main factors influencing the community structure of phyllosphere bacteria. This study highlights the key role of plant interspecific genetic relationships and plant attributes in shaping phyllosphere bacterial diversity.

The overlooked functions of trichomes: Water absorption and metal detoxication.

Trichomes are epidermal outgrowths on plant shoots. Their roles in protecting plants against herbivores and in the biosynthesis of specialized metabolites have long been recognized. Recently, studies are increasingly showing that trichomes also play important roles in water absorption and metal detoxication, with these roles having important implications for ecology, the environment, and agriculture. However, these two functions of trichomes have been largely overlooked and much remains unknown. In this review, we show that the trichomes of 37 plant species belonging to 14 plant families are involved in water absorption, while the trichomes of 33 species from 13 families are capable of sequestering metals within their trichomes. The ability of trichomes to absorb water results from their decreased hydrophobicity compared to the remainder of the leaf surface as well as the presence of special structures for collecting and absorbing water. In contrast, the metal detoxication function of trichomes results not only from the good connection of their basal cells to the underlying vascular tissues, but also from the presence of metal-chelating ligands and transporters within the trichomes themselves. Knowledge gaps and critical future research questions regarding these two trichome functions are highlighted. This review improves our understanding on trichomes.

[Community structure and species composition of typical Quercus variabilis natural secondary forest at the northern foothills of the Qinling Mountains, China]. / ç§¦å²­åŒ—éº“å ¸åž‹æ “çš®æ Žå¤©ç„¶æ¬¡ç”Ÿæž—ç¾¤è½ç»“æž„ä¸Žç‰©ç§ç»„æˆ.

We investigated species composition and community structure of a typical Quercus variabilis natural secondary forest in the northern foothills of the Qinling Mountains, within the dynamic monitoring plot of deciduous broad-leaved forest at the Louguantai experimental forest farm in Zhouzhi County, Shaanxi Province. The results showed that there were 3162 individual woody plants with diameter at breast height ≥1 cm in the plot, which were belonged to 42 species, 36 genera, and 25 families. The community genus's areal type was dominated by the temperate component, which accounted for 44.4%, and followed by the tropical component. The community was dominated by several tree species. The top three species with respect to importance value were Q. variabilis, Pinus tabuliformis, and Quercus aliena, with the sum of their importance value being 64.7%. The average DBH of all woody plants was 7.58 cm. The distribution of all individuals and dominant species in the tree layer was approximately normal, with more medium-size individuals. The community structure was stable. The community was poorly renewed, with a trend of population decline. Biodiversity indices varied considerably among different plots, being lower than those of subtropical broad-leaved evergreen forests. There was a significant correlation between community species distribution and environmental factors. Soil and topography explained 42.4% of the variation in community distribution. Altitude and soil alkali hydrolysable nitrogen had a significant effect on community distribution. Altitude, soil total phosphorus, and organic matter content significantly affected the species diversity of Q. variabilis communities. The stronger adaptability of Q. variabilis populations allowed them to become dominant in low-nutrient environments, which limited species diversity in the community.

Seasonal Influence of Biodiversity on Soil Respiration in a Temperate Forest.

Soil respiration in forests contributes to significant carbon dioxide emissions from terrestrial ecosystems but it varies both spatially and seasonally. Both abiotic and biotic factors influence soil respiration but their relative contribution to spatial and seasonal variability remains poorly understood, which leads to uncertainty in models of global C cycling and predictions of future climate change. Here, we hypothesize that tree diversity, soil diversity, and soil properties contribute to local-scale variability of soil respiration but their relative importance changes in different seasons. To test our hypothesis, we conducted seasonal soil respiration measurements along a local-scale environmental gradient in a temperate forest in Northeast China, analyzed spatial variability of soil respiration and tested the relationships between soil respiration and a variety of abiotic and biotic factors including topography, soil chemical properties, and plant and soil diversity. We found that soil respiration varied substantially across the study site, with spatial coefficients of variation (CV) of 29.1%, 27.3% and 30.8% in spring, summer, and autumn, respectively. Soil respiration was consistently lower at high soil water content, but the influence of other factors was seasonal. In spring, soil respiration increased with tree diversity and biomass but decreased with soil fungal diversity. In summer, soil respiration increased with soil temperature, whereas in autumn, soil respiration increased with tree diversity but decreased with increasing soil nutrient content. However, soil nutrient content indirectly enhanced soil respiration via its effect on tree diversity across seasons, and forest stand structure indirectly enhanced soil respiration via tree diversity in spring. Our results highlight that substantial differences in soil respiration at local scales was jointly explained by soil properties (soil water content and soil nutrients), tree diversity, and soil fungal diversity but the relative importance of these drivers varied seasonally in our temperate forest.

[Community structure of phyllosphere fungi associated with dominant tree species in a broad-leaved Korean pine forest of Changbai Mountain, Northeast China]. / é•¿ç™½å±±é˜”å¶çº¢æ¾æž—ä¼˜åŠ¿æ ‘ç§å¶é™ çœŸèŒç¾¤è½ç»“æž„.

Forest is the main component of terrestrial ecosystems that harbors about 40% of the existing species on the earth. As a vital component of biodiversity, phyllosphere microbes in the canopy play a critical and unique role in maintaining plant health, improving host resistance, and influencing global biogeochemical cycle. However, the studies on the community structure of phyllosphere fungi in natural forests are scarce as compared to that on rhizosphere microbes. Consequently, we know litter about how phyllosphere fungi associates with leaf traits. In this study, we analyzed fungal community composition of canopy leaves of six dominant tree species (i.e., Pinus koraiensis, Tilia amurensis, Quercus mongolica, Acer mono, Fraxinus mandshurica, and Ulmus japonica), in a broad-leaved Korean pine forest of Changbai Mountain Nature Reserve in Jilin Province, using high-throughput sequencing. We compared the differences of phyllosphere fungal community structure and functional groups of different dominant tree species. Moreover, 14 key leaf functional traits of their host trees were measured to investigate the relationships between fungal community composition and leaf functional traits. We found that the dominant phyla and class of phyllosphere fungi were Ascomycota and Basidiomycota, and Dothideomycetes and Taphrinomycetes, respectively. Results of LEfSe analysis indicated that all the tree species except Ulmus japonica had significant biomarkers, such as the Eurotiomycetes of Pinus koraiensis and the Ascomycetes of Quercus mongolica. The main functional groups of phyllosphere fungi were pathotroph. The results of redundancy and envfit analysis showed that functional traits related to plant nutrient acquisition as well as resistance to diseases and pests were the main factors influencing the community structure of phyllosphere fungi.

Disentangling the effects of species interactions and environmental factors on the spatial pattern and coexistence of two congeneric Pinus species in a transitional climatic zone.

Congeneric species are critical for understanding the underlying ecological mechanisms of biodiversity maintenance. Ecological mechanisms such as conspecific negative density dependence, species differences in life-history stages related to habitat preference, and limiting similarity are known to influence plant fitness, thereby influencing species coexistence and biodiversity. However, our understanding of these phenomena as they apply to coexistence among coniferous species is limited. We studied two congeneric Pinus species, Pinus armandii (PA) and Pinus tabulaeformis (PT), both of which are common pioneer species typically succeeded by oaks (Quercus), in a 25-ha warm temperate deciduous broad-leaved forest. Here, we addressed the following questions: (1) How do population structures and distributions patterns of these two Pinus species vary with respect to different life-history stages? (2) Does intra- and interspecific competition vary with respect to three life-history stages? And (3) What are the relative contributions of topographic and soil variables to the spatial distributions of the species across the three life-history stages? In addressing these questions, we utilized the pair-correlation function g(r), redundancy analysis (RDA), variance partitioning (VP), and hierarchical partitioning (HP) to identify habitat preferences and conspecific negative density dependence at different life-history stages from small to large trees. The results revealed that in both Pinus species, individuals in different life-history stages were subject to significant habitat heterogeneity, with a tendency for small trees to be distributed at higher latitudes that may be represents climate-change-driven migration in both species. In addition, the effects of conspecific negative density dependence on PT were stronger than those on PA due to limited dispersal in PT. Furthermore, we found that interspecific competition was weak due to the species differences in resource utilization and preference for key habitats. Our study shows that congeneric Pinus species avoids competition by exploiting distinct habitats and provides insight into forest community structure.

El Niño-Southern Oscillation affects the species-level temporal variation in seed and leaf fall in a mixed temperate forest.

El Niño-Southern Oscillation (ENSO), the variation between anomalously cold (La Niña) and warm conditions (El Niño), is one of the most prominent large-scale climate patterns with worldwide effects. Elevated seed and leaf fall has been found at the positive phase of ENSO (El Niño) in tropical forests. However, how seed and leaf fall respond to ENSO at species level is understudied, especially in temperate forests. In this study, we monitored seed and leaf fall at the species-level at 150 points across a 25-ha temperate forest in northeastern China over a span of 12 years. Using time series and wavelet analyses, we assessed three hypotheses: 1) temperate tree species' seed and leaf fall are strongly, but differently, correlated with ENSO and, 2) community synchrony in seed and leaf occurred both at seasonal and ENSO scales; finally, 3) local climatic modulated the effects of ENSO on seed and leaf fall. We found that ENSO was significantly correlated with seed and leaf fall of all species, although correlation strength varied across species (r = 0.206-0.658). Specifically, ENSO indices (ENSO12 or ENSO34) accounted for the most variation in seed and leaf fall of Acer pseudo-sieboldianum (40 % and 34 %, respectively) and ranged 4 %-31 % in all other species. Leaf fall was synchronous with ENSO cycles with a period of 2-7 years, but community synchrony of seed fall was only detected at seasonal scales. ENSO influenced seed fall of Fraxinus mandshurica and Tilla amurensis by mediating rainfall and relative humidity, respectively, highlighting the interactive effects of local climate and ENSO. Our findings highlight the potential effects of ENSO on ecosystems outside of tropical regions and improve our ability to predict regeneration dynamics and nutrient cycling of temperate forests under the context of global change.

Interannual climate variability has predominant effects on seedling survival in a temperate forest.

Mechanisms such as conspecific negative density dependence (CNDD) and niche partitioning have been proposed to explain species coexistence and community diversity. However, as a potentially important axis of niche partitioning, the role of interannual climate variability in driving local community dynamics remains largely unknown. Here we used a 15-year monitoring data set of more than 53,000 seedlings in a temperate forest to examine (1) what are the relative effects of interannual climate variability, biotic interactions, and habitat conditions on seedling survival; (2) how the effects of biotic interactions change with interannual climate variability, and habitat conditions; and (3) whether the impacts of interannual climate variability, biotic interactions, and habitat conditions differ with plant traits. Interannual climate variability accounted for the most variation in seedling survival at the community level, followed by biotic interactions, and habitat conditions. Increased snowpack and decreased minimum temperature during the non-growing season had positive effects on seedling survival. Effects of conspecific neighbor density were weakened in higher snowpack, effective accumulated temperature, elevation, and soil-resource gradient, but were intensified with increased ultraviolet radiation, maximum precipitation, minimum temperature, and soil moisture. In addition, the relative importance of interannual climate variability versus biotic interactions differed depending on species-trait groups. Specifically, biotic interactions for gravity-dispersed species had a larger effect size in affecting seedling survival than other trait groups. Also, gravity-dispersed species experienced a stronger CNDD than wind-dispersed, probably because wind-dispersed seedlings rarely had adult conspecifics nearby. We found that interannual climate variability was most strongly associated with seedling survival, but the magnitude of climatic effects varied among species-trait groups. Interannual climate variability may act as an inhibitor or accelerator to density-dependent interactions and should be accounted for in future studies, as both a potential direct and indirect factor in understanding the diversity of forest communities.

Demographic composition, not demographic diversity, predicts biomass and turnover across temperate and tropical forests.

The growth and survival of individual trees determine the physical structure of a forest with important consequences for forest function. However, given the diversity of tree species and forest biomes, quantifying the multitude of demographic strategies within and across forests and the way that they translate into forest structure and function remains a significant challenge. Here, we quantify the demographic rates of 1961 tree species from temperate and tropical forests and evaluate how demographic diversity (DD) and demographic composition (DC) differ across forests, and how these differences in demography relate to species richness, aboveground biomass (AGB), and carbon residence time. We find wide variation in DD and DC across forest plots, patterns that are not explained by species richness or climate variables alone. There is no evidence that DD has an effect on either AGB or carbon residence time. Rather, the DC of forests, specifically the relative abundance of large statured species, predicted both biomass and carbon residence time. Our results demonstrate the distinct DCs of globally distributed forests, reflecting biogeography, recent history, and current plot conditions. Linking the DC of forests to resilience or vulnerability to climate change, will improve the precision and accuracy of predictions of future forest composition, structure, and function.

Technologies and perspectives for achieving carbon neutrality.

Global development has been heavily reliant on the overexploitation of natural resources since the Industrial Revolution. With the extensive use of fossil fuels, deforestation, and other forms of land-use change, anthropogenic activities have contributed to the ever-increasing concentrations of greenhouse gases (GHGs) in the atmosphere, causing global climate change. In response to the worsening global climate change, achieving carbon neutrality by 2050 is the most pressing task on the planet. To this end, it is of utmost importance and a significant challenge to reform the current production systems to reduce GHG emissions and promote the capture of CO2 from the atmosphere. Herein, we review innovative technologies that offer solutions achieving carbon (C) neutrality and sustainable development, including those for renewable energy production, food system transformation, waste valorization, C sink conservation, and C-negative manufacturing. The wealth of knowledge disseminated in this review could inspire the global community and drive the further development of innovative technologies to mitigate climate change and sustainably support human activities.

Tree growth response to soil nutrients and neighborhood crowding varies between mycorrhizal types in an old-growth temperate forest.

Forest dynamics are shaped by both abiotic and biotic factors. Trees associating with different types of mycorrhizal fungi differ in nutrient use and dominate in contrasting environments, but it remains unclear whether they exhibit differential growth responses to local abiotic and biotic gradients where they co-occur. We used 9-year tree census data in a 25-ha old-growth temperate forest in Northeast China to examine differences in tree growth response to soil nutrients and neighborhood crowding between tree species associating with arbuscular mycorrhizal (AM), ectomycorrhizal (EM), and dual-mycorrhizal (AEM) fungi. In addition, we tested the role of individual-level vs species-level leaf traits in capturing differences in tree growth response to soil nutrients and neighborhood crowding across mycorrhizal types. Across 25 species, soil nutrients decreased AM tree growth, while neighborhood crowding reduced both AM and EM tree growth, and neither soil nor neighbors impacted AEM tree growth. Across mycorrhizal types, individual-level traits were stronger predictors of tree growth than species-level traits. However, most traits poorly mediated tree growth response to soil nutrients and neighborhood crowding. Our findings indicate that mycorrhizal types strongly shape differences in tree growth response to local soil and crowding gradients, and suggest that including plant-mycorrhizae associations in future work offers great potential to improve our understanding of forest dynamics.

Divergent above- and below-ground biodiversity pathways mediate disturbance impacts on temperate forest multifunctionality.

Biodiversity plays a fundamental role in provisioning and regulating forest ecosystem functions and services. Above-ground (plants) and below-ground (soil microbes) biodiversity could have asynchronous change paces to human-driven land-use impacts. Yet, we know very little how they affect the provision of multiple forest functions related to carbon accumulation, water retention capacity and nutrient cycling simultaneously (i.e. ecosystem multifunctionality; EMF). We used a dataset of 22,000 temperate forest trees from 260 plots within 11 permanent forest sites in Northeastern China, which are recovering from three post-logging disturbances. We assessed the direct and mediating effects of multiple attributes of plant biodiversity (taxonomic, phylogenetic, functional and stand structure) and soil biodiversity (bacteria and fungi) on EMF under the three disturbance levels. We found the highest EMF in highly disturbed rather than undisturbed mature forests. Plant taxonomic, phylogenetic, functional and stand structural diversity had both positive and negative effects on EMF, depending on how the EMF index was quantified, whereas soil microbial diversity exhibited a consistent positive impact. Biodiversity indices explained on average 45% (26%-58%) of the variation in EMF, whereas climate and disturbance together explained on average 7% (0.4%-15%). Our result highlighted that the tremendous effect of biodiversity on EMF, largely overpassing those of both climate and disturbance. While above- (ß = 0.02-0.19) and below-ground (ß = 0.16-0.26) biodiversity had direct positive effects on EMF, their opposite mediating effects (ß = -0.22 vs. ß = 0.35 respectively) played as divergent pathways to human disturbance impacts on EMF. Our study sheds light on the need for integrative frameworks simultaneously considering above- and below-ground attributes to grasp the global picture of biodiversity effects on ecosystem functioning and services. Suitable management interventions could maintain both plant and soil microbial biodiversity, and thus guarantee a long-term functioning and provisioning of ecosystem services in an increasing disturbance frequency world.

Context-dependency of tree species diversity, trait composition and stand structural attributes regulate temperate forest multifunctionality.

High species diversity is generally thought to be a requirement for sustaining forest multifunctionality. However, the degree to which the relationship between species-, structural-, and trait-diversity of forests and multifunctionality depend on the context (such as stand age or abiotic conditions) is not well studied. Here, we hypothesized that context-dependency of tree species diversity, functional trait composition and stand structural attributes promote temperate forest multifunctionality including above- and below-ground multiple and single functions. To do so, we used repeated forest inventory data, from temperate mixed forests of northeast China, to quantify two above-ground (i.e. coarse woody productivity and wild edible plant biomass), five below-ground (i.e. soil organic carbon, total soil nitrogen, potassium, phosphorus and sulfur) functions, tree species diversity, individual tree size variation (CVDBH) and functional trait composition of specific leaf area (CWMSLA) as well as stand age and abiotic conditions. We found that tree species diversity increased forest multifunctionality and most of the single functions. Below-ground single and multifunctionality were better explained by tree species diversity. In contrast, above-ground single and multifunctionality were better explained by CVDBH. However, CWMSLA was also an additional important driver for maintaining above- and below-ground forest multifunctionality through opposing plant functional strategies. Stand age markedly reduced forest multifunctionality, tree species diversity and CWMSLA but substantially increased CVDBH. Below-ground forest multifunctionality and tree species diversity decreased while above-ground forest multifunctionality increased on steep slopes. These results highlight that context-dependency of forest diversity attributes might regulate forest multifunctionality but may not have a consistent effect on above-ground and below-ground forest multifunctionality due to the fact that those functions were driven by varied functional strategies of different plant species. We argue that maximizing forest complexity could act as a viable strategy to maximizing forest multifunctionality, while also promoting biodiversity conservation to mitigate climate change effects.

Tree species traits affect which natural enemies drive the Janzen-Connell effect in a temperate forest.

A prominent tree species coexistence mechanism suggests host-specific natural enemies inhibit seedling recruitment at high conspecific density (negative conspecific density dependence). Natural-enemy-mediated conspecific density dependence affects numerous tree populations, but its strength varies substantially among species. Understanding how conspecific density dependence varies with species' traits and influences the dynamics of whole communities remains a challenge. Using a three-year manipulative community-scale experiment in a temperate forest, we show that plant-associated fungi, and to a lesser extent insect herbivores, reduce seedling recruitment and survival at high adult conspecific density. Plant-associated fungi are primarily responsible for reducing seedling recruitment near conspecific adults in ectomycorrhizal and shade-tolerant species. Insects, in contrast, primarily inhibit seedling recruitment of shade-intolerant species near conspecific adults. Our results suggest that natural enemies drive conspecific density dependence in this temperate forest and that which natural enemies are responsible depends on the mycorrhizal association and shade tolerance of tree species.

Ectomycorrhizal fungus-associated determinants jointly reflect ecological processes in a temperature broad-leaved mixed forest.

Ectomycorrhizal (ECM) fungi are closely related to vegetation compositions, edaphic properties, and site-specific processes. However, the coevolutionary mechanisms underlying the spatial distributions in floristic and ECM fungal composition in the context of biotic adaptations and abiotic variances remain unclear. We combine a total of 25 ECM fungus-associated environmental variables to impose three types of composite scores and then quantify the environmental gradients of geographical site, soil chemical property and vegetation functional trait across 122 grids of 20 m × 20 m in a 25-hm2 forest plot. Significant dissimilarities in vegetational and ECM fungal abundance and composition existed along the above environmental gradients. Specifically, a contrasting floristic distribution (e.g., Betula platyphylla vs. Tilia mandshurica) existed between the northeastern and southwestern areas and was closely related to the nutrient and moisture gradients (with high levels in the west and low levels in the east). Furthermore, the ECM fungal communities were more abundant in the nutrient-poor and low-moisture environments than in the nutrient-rich and high-moisture environments, and the mixed-forest in the middle-gradient sites between the northeastern and southwestern areas harbored the highest ECM fungal diversity. These findings suggest that predictable within-site vegetation succession is closely related to ECM-associated determinants and the natural spatial heterogeneity of edaphic properties at a local scale.

Temporal stability of aboveground biomass is governed by species asynchrony in temperate forests.

Understanding the effects of plant species diversity and trait composition on aboveground biomass is a central focus of ecology and has important implications for biodiversity conservation. However, the simultaneous direct and indirect effects of soil nutrients, species asynchrony, functional trait diversity, and trait composition for explaining the community temporal stability of aboveground biomass remain underrepresented in natural forests. Here, we hypothesized that species asynchrony relative to soil nutrients, functional trait diversity, and trait composition plays a central role in stabilizing the community temporal stability of natural forests. We tested this hypothesis using a structural equation model based on 10-year continuous monitoring data (i.e., three-time repeated forest inventories) in both second-growth and old-growth temperate forests in northeast China. Our results showed that the community temporal stability of aboveground biomass was driven by a strong direct positive effect of species asynchrony in both second-growth and old-growth temperate forests, whereas functional trait diversity and composition (i.e. community-weighted mean of leaf nitrogen content) were of additional importance in an old-growth forest only. Functional trait diversity decreased community-weighted mean of leaf nitrogen content in an old-growth forest, whereas this relationship was non-significant in a second-growth forest. Soil nutrients had non-significant effects on the community temporal stability of both second-growth and old-growth forests. Species asynchrony was the direct determinant of the community temporal stability of aboveground biomass in temperate forests. The direct effect of species asynchrony increased with forest succession, implying that temporal niche differentiation and facilitation increase over time. This study suggests that managing forests with mixtures of both early and late successional species or shade intolerant and tolerant species, not only species diversity, is important for maintaining forest stability in a changing environment. We argue that the species asynchrony effect is crucial to understand the underlying ecological mechanisms for a diversity-biomass relationship in natural forests.