ABSTRACT
PURPOSE: The repressor element 1 (RE-1) silencing transcription factor (REST) is a transcription factor which represses the expression of neuronal differentiation-related genes including SYN1 gene. CoREST, encoded by RCOR1 gene, binds to the REST protein for remodeling of chromatin structure. Although there is a relation among REST, RCOR1, and SYN1 genes, the role of these genes in glioma tumors is still unclear. In this study, expressions of REST, RCOR1, and SYN1 genes were detected in primary cultures derived from tumor samples of diffuse astrocytoma (DA), anaplastic oligodendroglioma (AO), and glioblastoma multiforme (GBM) cases. METHODS: Expression profiles were analysed by RT-qPCR and the copy number variations were examined with qPCR in primary cultures. ChIP assay was performed to show binding characteristics of REST and CoREST proteins on promoter region of SYN1 gene. RESULTS: Means of relative expression for REST were as follows: 0.7898, 0.7606, and 0.7318 in DA, AO, and GBM groups, respectively. For RCOR1, expression means in DA, AO, and GBM groups were 0.7203, 0.7334, and 0.7230, respectively. SYN1 expression means were as follows: 0.3936, 0.3192, and 0.3197 in DA, AO, and GBM groups, respectively. Neither gain nor loss of copy numbers were detected for REST and RCOR1 genes in all groups. Copy loss for SYN1 was detected in primary culture of a DA case. REST and CoREST presented positive precipitation pattern on promoter region of SYN1 gene. CONCLUSIONS: Expressions of REST and RCOR1 genes may downregulate SYN1 expression in gliomas. Low expression pattern of SYN1 may maintain cancer stem-like phenotype which contributes to development of gliomas.
Subject(s)
Co-Repressor Proteins/genetics , Glioma/genetics , Nerve Tissue Proteins/genetics , Repressor Proteins/genetics , Synapsins/genetics , Transcriptome/genetics , Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young AdultABSTRACT
In this study, we reported on the synthesis and biological evaluation of radiolabeled fluorescent dye conjugated bovine serum albumin nanoparticles within the size range 190-210 nm. The bovine serum albumin nanoparticles (BSANPs) were prepared using a desolvation method, and chemical cross-linking was performed using gluteraldehyde. Furthermore, pheophorbide-a (PH-A) was loaded on the BSANPs. The results obtained from dynamic light scattering and electron microscopy have proved that nanoparticles are highly monodisperse and near-spherical shaped. The photo-physical properties of the PH-A-BSANPs were obtained using the spectrophotometric techniques. According to the results, PH-A and BSANPs show high non-covalent interaction. PH-A loaded nanoparticles were labeled with (99m)Tc and the radio-labeling efficiency was determined as 90 ± 1.2%. Biodistribution studies of (99m)Tc labeled PH-A-BSANPs and PH-A were carried out using female Albino Wistar rats, and (99m)Tc-PH-A-BSANPs showed a significantly higher uptake in the breast and uterus than (99m)Tc-PH-A. Cell culture study was carried out in MCF-7 cell line (human breast adenocarcinoma cell line). According to the cell culture studies, (99m)Tc-PH-A-BSANPs showed a higher uptake than (99m)Tc-PH-A. Moreover, PH-A-BSANPs demonstrated good photo-physical properties and BSANPs increased the uptake of PH-A on to the MCF-7 cell line. These results confirm that (99m)Tc labeled PH-A-BSANPs could be utilized for radioimaging.
Subject(s)
Chlorophyll/analogs & derivatives , Drug Carriers , Molecular Imaging/methods , Nanoparticles , Radiation-Sensitizing Agents/pharmacokinetics , Radiopharmaceuticals/pharmacokinetics , Serum Albumin, Bovine/pharmacokinetics , Technetium/pharmacokinetics , Adenocarcinoma/metabolism , Adenocarcinoma/pathology , Animals , Breast Neoplasms/metabolism , Breast Neoplasms/pathology , Cell Line, Tumor , Cell Survival/drug effects , Chlorophyll/chemistry , Chlorophyll/pharmacokinetics , Chlorophyll/toxicity , Cross-Linking Reagents/chemistry , Female , Glutaral/chemistry , Humans , Light , Magnetic Resonance Spectroscopy , Mass Spectrometry , Microscopy, Electron , Microscopy, Fluorescence , Particle Size , Radiation-Sensitizing Agents/chemistry , Radiation-Sensitizing Agents/toxicity , Radiopharmaceuticals/chemistry , Rats , Rats, Wistar , Scattering, Radiation , Serum Albumin, Bovine/chemistry , Spectrophotometry, Ultraviolet , Technetium/chemistry , Tissue DistributionABSTRACT
Radiopharmaceuticals are useful to evaluate effectiveness of cancer treatments as well as diagnosis of diseases. (99m)Tc-Glucoheptonate has high sensitivity for imaging lung cancer tissues. In this study, the potential use of (99m)Tc-glucoheptonate for monitoring apoptosis related to chemotherapeutic agents is investigated in vitro using A549 lung cancer cell line. A decrease in (99m)Tc-glucoheptonate uptake ratio was observed depending on the level of apoptosis. (99m)Tc-glucoheptonate is found to be useful for the detection of apoptosis following treatment in A549 lung tumor cells.
