Subject(s)
Chromosomes, Mammalian/genetics , Insulin/metabolism , Membrane Proteins/genetics , Radiation Hybrid Mapping , Sus scrofa/genetics , Animals , Base Sequence , Cloning, Molecular , DNA Primers , DNA, Complementary/genetics , Membrane Proteins/metabolism , Molecular Sequence Data , Pseudogenes/genetics , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNAABSTRACT
OBJECTIVE: To explore the therapeutical mechanism of Bushen Shengjing Decoction (BSSJD) in treating infertility. METHODS: Fed Wistar male rats with adenine 30 mg/100 g.d continuously for 30 days. The rats manifested the symptom which was similar to the human Kidney Yang Deficiency, the genitality dropped, the function of sperm-producing in the testis was disturbed, the contents of luteinizing hormone (LH), testosterone (T) and compound F (F) in blood were all lower than rats of normal group, P < 0.01. The model rats took the BSSJD 3 ml/100 g.d (each ml contains crude drugs 2 g) continuously for 10 days, interval for three days, then medicated again and total medication of BSSJD was 30 days. RESULTS: The symptom of Kidney Yang Deficiency in rats was improved obviously, the genitality, sperm quality and function of sperm producing in testis were all enhanced obviously, so did the hormone level of LH, T, F in blood. Comparing to untreated control group, the difference was significant (P < 0.05). CONCLUSION: BSSJD has the function of reinforcing Yang and replenishing essence and regulate the endocrinological function.
Subject(s)
Drugs, Chinese Herbal/therapeutic use , Testicular Diseases/drug therapy , Yang Deficiency/drug therapy , Adenine , Animals , Fertility Agents, Male/therapeutic use , Male , Random Allocation , Rats , Rats, Wistar , Sperm Motility/drug effects , Yang Deficiency/chemically inducedABSTRACT
Eighty-seven cases of male infertility with semen abnormality were treated and observed by Bushen Shengjing Pill, its curative effect was evaluated with quantitative assessment and analytical comparison comprehensive scoring of semen routine analysis. These patients were treated for one to three courses of treatment, the semen quality was enhanced obviously, the comprehensive semen routine analysis score was enhanced significantly (P < 0.001) as compared with that before treatment, the spouse pregnant rate was 56.32% (49/87), and total effective rate was 95.40% (83/87). The result showed that this prescription had bidirectional regulatory function in folliclestimulating hormone, luteotropic hormone, testosterone, corticosterone, and could make the enhanced or reduced hormone level to normal value.
