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Assembly of microarrays for genome-wide measurement of DNA copy number.

Snijders, A M; Nowak, N; Segraves, R; Blackwood, S; Brown, N; Conroy, J; Hamilton, G; Hindle, A K; Huey, B; Kimura, K; Law, S; Myambo, K; Palmer, J; Ylstra, B; Yue, J P; Gray, J W; Jain, A N; Pinkel, D; Albertson, D G.

Nat Genet ; 29(3): 263-4, 2001 Nov.

Article in English | MEDLINE | ID: mdl-11687795

ABSTRACT

We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.

Subject(s)

Aneuploidy , Gene Dosage , Genome, Human , Genomics/methods , Oligonucleotide Array Sequence Analysis/methods , Chromosomes, Artificial, Bacterial/genetics , Cloning, Molecular , Female , Humans , Male , Polymerase Chain Reaction , Polyploidy , Tumor Cells, Cultured , X Chromosome/genetics

ABSTRACT

Subject(s)

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