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1.
Comput Intell Neurosci ; 2022: 8006605, 2022.
Article in English | MEDLINE | ID: mdl-36120696

ABSTRACT

With the continuous development of network technology, the production and lifestyle of human beings are also quietly changing. People find that the network makes life faster and more convenient and also makes our life more "transparent." The openness, sharing, and convenience of the network make it easier to infringe on the privacy rights of others, bringing an unprecedented impact on traditional privacy protection. This paper studied information security assurance-related algorithms and built an information security assurance model. Through this model, some privacy violation events existing in the network today were analyzed. Then, a corresponding questionnaire was constructed according to some of the problems of these events. The questionnaire is mainly used for research on people of all age groups, mainly to study their understanding of relevant legal knowledge and their views on the law. A total of 188 valid questionnaires were collected in this survey. Only 37.23% people knew a lot about the law. Most of them learned it from the Internet, and they rarely learned about it from relevant books. About 70% people believe that the law plays a big role in privacy protection, and they will choose legal help if they encounter privacy violations. However, this is inconsistent with some data obtained from the information security assurance model. Through analysis, it can be seen that although they want legal help, they lack the relevant knowledge of the law, and they do not know whether the privacy of individuals has been violated. Therefore, it is necessary to strengthen personal knowledge of privacy protection laws and improve the personal information legal protection and fair use system.


Subject(s)
Confidentiality , Privacy , Algorithms , Computer Security , Humans , Surveys and Questionnaires
2.
Chem Commun (Camb) ; 50(69): 9907-9, 2014 Sep 07.
Article in English | MEDLINE | ID: mdl-25030218

ABSTRACT

A surface-enhanced Raman scattering (SERS) method has been proposed for simultaneous detection and imaging of two biomarkers on the surface of a single human breast cancer cell (MCF-7) by self-assembly of branched DNA-gold nanoaggregates.


Subject(s)
Biomarkers, Tumor/analysis , Breast Neoplasms/diagnosis , DNA , Gold , Metal Nanoparticles , Spectrum Analysis, Raman/methods , Breast/pathology , DNA/chemistry , Diagnostic Imaging , Female , Gold/chemistry , Humans , MCF-7 Cells , Metal Nanoparticles/chemistry , Metal Nanoparticles/ultrastructure
3.
Anal Chim Acta ; 756: 73-8, 2012 Dec 05.
Article in English | MEDLINE | ID: mdl-23176741

ABSTRACT

A PCR (polymerase chain reaction)-free electrochemiluminescence (ECL) strategy based on aptamers and ECL nanoprobes was developed for rapid collection and detection of Ramos cells. The ECL nanoprobes consisted of gold nanoparticles (AuNPs), linker DNA and tris-(2,2'-bipyridyl) ruthenium (TBR)-labeled signal DNA. The linker DNA and signal DNA were modified on the surface of the AuNPs through AuS bonds. The linker DNA can hybridize partly with the aptamers loaded on the magnetic beads to construct the magnetic biocomplex. In the presence of the cancer cells, the aptamers conjugated with the cancer cells with higher affinity. The ECL nanoprobe released from the biocomplex and subsequently hybridized with the capture DNA modified on the Au electrode. The ECL intensity of the TBR loaded on the nanoprobes directly reflected the amount of the cancer cells. With the use of the developed ECL probe, a limit of detection as low as 50 Ramos cells per mL could be achieved. The proposed methods based on ECL should have wide applications in the diagnosis of cancers due to their high sensitivity, simplicity and low cost.


Subject(s)
Aptamers, Nucleotide/chemistry , Biosensing Techniques/instrumentation , Gold/chemistry , Metal Nanoparticles/chemistry , Neoplasms/diagnosis , Biosensing Techniques/economics , Biosensing Techniques/methods , Cell Line, Tumor , Humans , Limit of Detection , Luminescent Measurements
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