ABSTRACT
Implantable cervical vagus nerve stimulation (iVNS) is a representative and promising neuromodulation. However, the invasive nature restricts its application. Traditional auricular acupuncture treatment has a long history. The auricular branch of the vagus nerve (ABVN) is a branch on the surface of the ear. Some studies demonstrates that transcutaneous auricular vagus nerve stimulation (taVNS) would achieve similar effects as iVNS. TaVNS and iVNS share a common anatomical basis and acting mechanism. In this article, we made a comparison between iVNS and taVNS in indications and efficacy. The recent studies have revealed similar clinical efficacy of taVNS, taVNS would expand the indication of iVNS. High-quality clinical evidences are needed before taVNS become be an alternative of iVNS.
Subject(s)
Acupuncture, Ear , Transcutaneous Electric Nerve Stimulation , Vagus Nerve Stimulation , Humans , Vagus Nerve/physiology , Treatment OutcomeABSTRACT
Evidence suggests that superoxide dismutase 1 (SOD1) promotes glucose vs lipid metabolism depending on the diet type. We recently reported that nanoformulated SOD1 (Nano) improved lipid metabolism without altering glucose homeostasis in high-fat (HF) diet-fed mice. Here, we sought to determine the effects and potential mechanisms of Nano in modulating glucose and lipid homeostasis in mice fed a normal chow diet (CD) vs HF diet. Mice were fed a CD or a HF diet (45%) for 10 wk and injected with Nano once every 2 days for 15 days. The fasting glucose level was lower (P < 0.05) in CD + Nano-treated mice compared to control. Conversely, blood glucose was not altered but serum triglycerides were lower in HF + Nano-treated mice. Genes involved in fatty acid synthesis were reduced by Nano in the skeletal muscle of CD but not of HF diet-fed mice. Adenosine monophosphate-activated protein kinase (AMPK), which promotes both glucose and lipid metabolism depending on the fuel availability, is activated by Nano in CD-fed mice. Moreover, Nano increased phosphorylation of ACC, a downstream target of AMPK, in both CD and HF diet-fed mice. Nano increased mitochondrial respiration in C2C12 myocytes in the presence of glucose or fatty acid, and this effect is inhibited by Compound C, an AMPK inhibitor. Our data suggest that Nano promotes glucose and lipid metabolism in CD and HF diet-fed mice, respectively, and this effect is mediated partly via AMPK signaling.
Subject(s)
AMP-Activated Protein Kinases/metabolism , Blood Glucose/drug effects , Diet, High-Fat/adverse effects , Glucose/metabolism , Nanostructures , Superoxide Dismutase-1/pharmacology , AMP-Activated Protein Kinases/genetics , Animals , Cell Line , Gene Expression Regulation, Enzymologic , Homeostasis , Lipid Metabolism , Mice , Myoblasts/metabolism , Oxidative Stress , Signal Transduction , Superoxide Dismutase-1/administration & dosageABSTRACT
Objective To explore the pathogenic effect of serum platelet derived growth factor(PDGF) in Henoch - Schonlein pur-pura nephritis(HSPN) and the influence of heparin. Methods The levels of serum PDGF were detected before and after heparin therapy by double antibody ELISA in the peripheral blood of patients. All patients were injected with heparin calcium[100 U/(kg?d)] for two to three weeks. Results The levels of serum PDGF(596.23 ? 199.43) ng/L in 30 HSPN cases before heparin therapy were significantly higher than those of the control group(259.76?69.58) ng/L (P
