Establishment of the two-dimensional gel electrophoretic protein map of the human sperm head / 中华男科学杂志

<p><b>OBJECTIVE</b>To construct the two-dimensional electrophoretic (2-DE) protein map of the human sperm head.</p><p><b>METHODS</b>Protein extracts of the normal human sperm and sperm head were loaded on an 18 cm immobilized pH gradients (IPG) strip holder and separated with isoelectric focusing electrophoresis as the first dimension, and then with upright SDS-PAGE as the second. Different protein spots between them were compared with Image Master 3.0, and so were the maps of method I with Urea/Thiourea/Guanidine HCl and method II with Kit/Guanidine HCl.</p><p><b>RESULTS</b>Of the whole-sperm proteome, 802 protein spots were obtained by method I, and 797 by method II. Among them, distribution patterns of 492 spots were the same. A sperm protein map was constructed with 1107 protein spots after the interaction of the spots obtained by both the methods. Of the sperm-head proteome, 428 protein spots were obtained, all found in the whole-sperm protein map after comparison.</p><p><b>CONCLUSION</b>Both the methods could be complementarily used to construct a sperm protein map, and the map could serve as a model for the establishment of the sperm protein profile.</p>

Chromosome Analysis of 108 Primary and Secondary Amenorrhea Patients / 中国医师杂志

Objective To analyze the chromosome abnormalities in the patients with primary and secondary amenorrhea in the infertility clinic, and provide the guidance for their reproduction.Methods Peripheral blood lyphocytes chromoseme samples were prepared and the karyotype was analyzed according to routine method.Results 24 abnormal chromosome karyotypes were detected in 108 cases, the abnormality detection rate was 22.2%, and it was mainly involved in the numerical or structural abnormality of chromosome X. Conclusions Chromosome abnormality is an important cause for primary and secondary amenorrhea, and the X chromosome abnormality is involed mainly.For those patients,chromosome examination should routinely be taken before assistant reproductive technique.