Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Biochem Biophys Res Commun ; 313(4): 998-1003, 2004 Jan 23.
Article in English | MEDLINE | ID: mdl-14706641

ABSTRACT

Renal tubular citrate transport is accomplished by electrogenic Na(+) coupled dicarboxylate transporter NaDC-1, a carrier subjected to regulation by acidosis. Trafficking of the Na(+)/H(+) exchanger NHE3 is controlled by NHE regulating factors NHERF-1 and NHERF-2 and the serum and glucocorticoid inducible kinase SGK1. To test for a possible involvement in NaDC-1 regulation, mRNA encoding NaDC-1 was injected into Xenopus oocytes with or without cRNA encoding NHERF-1, NHERF-2, SGK1, SGK2, SGK3, and/or the constitutively active form of the related protein kinase B ((T308,S473D)PKB). Succinate induced inward currents (I(succ)) were taken as a measure of transport rate. Coexpression of neither NHERF-1 nor NHERF-2 in NaDC-1 expressing oocytes significantly altered I(succ). On the other hand, coexpression of SGK1, SGK3, and (T308,S473D)PKB stimulated I(succ), an effect further stimulated by additional coexpression of NHERF-2 but not of NHERF-1. The action of the kinases and NHERF-2 may link urinary citrate excretion to proximal tubular H(+) secretion.


Subject(s)
Cytoskeletal Proteins/metabolism , Dicarboxylic Acid Transporters/metabolism , Kidney/metabolism , Nuclear Proteins , Organic Anion Transporters, Sodium-Dependent/metabolism , Protein Serine-Threonine Kinases/metabolism , Proto-Oncogene Proteins/metabolism , Symporters/metabolism , Animals , Citric Acid/metabolism , Cytoskeletal Proteins/genetics , Dicarboxylic Acid Transporters/genetics , Female , Humans , Immediate-Early Proteins , In Vitro Techniques , Kidney Tubules, Proximal/metabolism , Kinetics , Oocytes/drug effects , Oocytes/metabolism , Organic Anion Transporters, Sodium-Dependent/genetics , Phosphoproteins/genetics , Phosphoproteins/metabolism , Protein Serine-Threonine Kinases/genetics , Proto-Oncogene Proteins/genetics , Proto-Oncogene Proteins c-akt , Rats , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Sodium-Hydrogen Exchangers , Succinic Acid/pharmacology , Symporters/genetics , Xenopus laevis
SELECTION OF CITATIONS
SEARCH DETAIL
...