Doxycycline-Loaded Nitric Oxide-Releasing Nanomatrix Gel in Replanted Rat Molar on Pulp Regeneration.

The aim of the present study was to evaluate the effect of doxycycline-loaded NO-releasing nanomatrix gel on pulp regeneration in replantation of avulsed rat teeth. A total of 28 maxillary first molars extracted from rats were replanted. The rats were divided into two groups based on the use of root surface treatment: doxycycline-loaded NO-releasing nanomatrix group and no treatment. Eight weeks after replantation, the rats were sacrificed, and the teeth were evaluated using histomorphometric analysis. On histomorphometric analysis, the NO-releasing nanomatrix group demonstrated a significantly lower grade of pulp inflammation (1.00 ± 1.11, mean ± standard deviation) compared to the no treatment group (2.21 ± 1.25, p = 0.014). NO-releasing nanomatrix group showed a significantly higher grade of pulp regeneration (2.57 ± 0.85, p = 0.012) and significantly lower grade of pulp inflammation (1.00 ± 0.68, p = 0.025) compared to the no treatment group. In conclusion, NO-releasing nanomatrix gel improved pulp regeneration of replanted teeth, though the sample size of this study was rather small. Within the limits of this study, NO-releasing nanomatrix gel can provide more favorable pulpal regeneration despite replantation.

Analysis of bacterial community profiles of endodontically infected primary teeth using pyrosequencing.

OBJECTIVE: The purpose of this study was to identify the endodontic microbiome in primary teeth with dental caries using high-throughput pyrosequencing and to establish data on the oral microbiome of primary teeth with endodontic infection. METHODS: Ten primary teeth with dental caries and endodontic infection were included. Samples were collected from root canals using sterilized paper points and analyzed by pyrosequencing, based on the V1-V3 hypervariable region of the 16S rRNA gene. The data were analyzed using the CLcommunity software. RESULTS: Analysis of the 10 samples yielded a total of 64,291 16S rRNA gene sequences. In total, 1586 OTUs (range, 91-235), six bacterial phyla, including unclassified, and 187 genera were assigned. At the genus level, Neisseria (group A), Acinetobacter (group B), and Fusobacterium (group C) were prominent. These predominant microorganisms were associated with the clinical condition and reflected the progression of endodontic infection in primary teeth. CONCLUSIONS: This study revealed a relationship between the oral microbiome and endodontic infection in primary teeth. Additionally, anaerobic bacteria such as Fusobacterium species were dominant in the teeth with apical abscesses.

Characteristics of Mesiodens and Its Related Complications.

PURPOSE: The purpose of this study was to evaluate the effect of mesiodens on adjacent permanent anterior teeth according to mesiodens characteristics. METHODS: To evaluate complications associated with mesiodens, according to its characteristics, a total of 107 children were studied who had a chief complaint of mesiodens. The number, morphology, direction of eruption, position of mesiodens, root developmental stage and complications related to adjacent permanent maxillary central incisors (APMCIs) were evaluated by radiographic imaging. RESULTS: The final sample was 107 children and total number of mesiodens was 153. Among these patients, 67 percent showed clinical complications. The primary morphologic type was conical (96.1 percent) and the most common direction of eruption was inverted (56.2 percent). Most mesiodens (80.4 percent) were located on the palatal side of the APMCIs. CONCLUSIONS: The direction of mesiodens and the eruption period of adjacent permanent incisors affected the complication rate. The positional relationship between mesiodens and adjacent permanent central incisors had the strongest influence on both eruption disturbance and displacement of permanent central incisors.