Fucoidan extract from brown seaweed (Sargassum echinocarpum): molecular weight, elemental composition, selectivity and anticancer activity against breast cancer cells.

Molecular weight and elemental composition contribute to fucoidan bioactivity. Fucoidan extract from S. echinocarpum had three fractions varying in molecular weights (Mw), whereas crude fucoidan extract had a moderate Mw of 2,034.31 kDa. The fucoidan extract contained several elements, including C (38.19%), O (44.46%), and S (2.61%). Moreover, the fucoidan extract exhibited toxicity to breast cancer cells (MCF-7) at 297.58 ± 2.40 ppm. The extract induced apoptosis by 49.78%, 72.05%, and 89.35% after incubation for 24, 48, and 72 h, respectively. Fucoidan increased MDA levels in MCF-7 cells, indicating its anticancer properties through the induction of apoptosis-induced lipid peroxidation. Furthermore, the anticancer activity of fucoidan extract was not significantly different from the standard fucoidan (F. vesiculosus). In addition, the extract was selective and non-toxic to human normal cells (TIG 1-20), indicating its safety for consumption and potential as an anticancer agent derived from marine algae.

Characterization of 'novel fucoidan' extracted from brown seaweed (Sargassum echinocarpum J. Ag.) using Ultrasound-Assisted Extraction (UAE) and its potential antioxidant activity.

Fucoidan of Sargassum echinocarpum was extracted using the UAE method. This method produced a fucoidan extract with a yield of 2.82-3.93% and antioxidant activity of 44.57-98.77 ppm at different temperatures and extraction times. The total sulphate, fucose, phenolic, protein, and uronic acid contents were 5.50-6.24%, 17.22-63.22%, 14.66-78.20 mgGAE/g, 0-14.42 mg/g, and 191.43-353.76 mg/g, respectively. Furthermore, extraction treatment with UAE also affected the topology and morphology of the fucoidan extract. The analysis results showed the presence of monosaccharides such as galactose 1.37%, mannose 0.642%, and xylose 2.86%, while glucose and rhamnose were not detected. The extract also had the same absorption band as the fucose and fucoidan standard (F. vesiculosus) as well as a similar basic skeletal structure. Based on the experimental results, the fucoidan extract had excellent antioxidant properties.

In silico molecular docking, molecular dynamics, ADMET analysis of fucoidan against receptor frizzled-8 and coreceptor LRP6 in Wnt/ß-Catenin pathway and in vitro analysis of fucoidan extract from Sargassum echinocarpum as ß-catenin inhibitor in breast cancer cell line (MCF-7).

This study aimed to investigate the effect of fucoidan on the Wnt/ß-Catenin pathway using both in-silico molecular docking, molecular dynamics, ADMET analysis (in frizzled-8 receptor and LRP6 coreceptor) and in-vitro experiments using MCF-7 breast cancer cells. Through the molecular docking analysis, the binding energies on the frizzled-8 receptor were -5.6, -5.1, -9.4, and -8.8 kcal/mol, respectively. Meanwhile, those on the LRP6 receptor, were -7.3, -6.2, -10.0, and -9.8 kcal/mol, respectively. The results showed that fucoidan had a favorable binding affinity for both receptors. Furthermore, it was discovered to reduce the interaction and binding affinity between Wnt agonists to frizzled-8 and LRP6 receptors. This reduction was reflected in the change in the binding energy of the fucoidan-Wnt agonist-frizzled 8 and fucoidan-Wnt agonist-LRP6 complexes, which exhibited decreases of -7.0 kcal/mol and -7.8 kcal/mol, respectively. Fucoidan was found stable in complexes with frizzled-8 receptor and co-receptor LRP6. ADMET study showed it's non-carcinogenic and can be distributed in the body. Fucoidan effectively inhibited ß-catenin production, a critical factor in the Wnt/ß-catenin pathway. The MCF-7 breast cancer cells were treated with fucoidan extract from S. echinocarpum at incubation times of 24, 48, and 72 h, resulting in a reduction of ß-catenin levels by 95.19%, 83.88%, and 80.88%, respectively. Fucoidan also shows no significant difference in value compared to fucoidan standard (F. vesiculosus) and doxorubicin. Fucoidan exhibited antiproliferative effects against breast cancer cells, specifically through its modulation of the Wnt/ß-Catenin pathway, and held great potential as an herbal anticancer agent.Communicated by Ramaswamy H. Sarma.

Genotoxicity in the rivers from the Brantas catchment (East Java, Indonesia): occurrence in sediments and effects in Oreochromis niloticus (Linnæus 1758).

This paper reports the first data from an integrated study investigating genotoxicity in the Brantas River, Java, Indonesia. Results showed that organic sediment extracts from the sites in the Brantas Delta retained genotoxic compounds identified using the SOS Chromotest and that the Aloo River and, to a lesser extent, the Surabaya River were the most contaminated studied sites. This genotoxicity was attributable to compounds that did not require any bioactivation under the test conditions. Occurrence of genotoxic effects was further investigated in erythrocytes from Nile tilapia, Oreochromis niloticus. High numbers of micronuclei were counted, especially in fish sampled in the rivers of the Brantas Delta. Moreover, cytoplasmic alterations which could be indicative of the presence of lipofuscin were found in the cytoplasm of the fish blood cells, especially in fish from the Aloo, Surabaya and Kalimas rivers. Altogether, our data showed that genotoxicity is occurring in fish living in rivers of the delta of the Brantas River and suggest that sediments from these sites may constitute a major source of pollution and hazard for species living or feeding in the area.