ABSTRACT
This is the first report showing that rotavirus infects the urinary sediment cells in immunocompetent children with rotavirus gastroenteritis. We found that inclusion-bearing cells were frequently detected in the urine samples of patients with rotavirus gastroenteritis. These cells were positive for cytokeratin, which was sometimes coexpressed with rotavirus antigen, in our immunohistochemical analysis. Moreover, in nested RT-PCR experiments, we detected rotavirus double-stranded RNA in some urine samples of patients with rotavirus gastroenteritis. We concluded that rotavirus could lead to infection of the urinary sediment cells concomitantly with rotavirus gastroenteritis.
Subject(s)
Epithelial Cells/virology , Gastroenteritis/virology , Kidney Tubules/cytology , RNA, Viral/isolation & purification , Rotavirus Infections/virology , Rotavirus/genetics , Rotavirus/isolation & purification , Urine , Antigens, Viral/metabolism , Child , Child, Preschool , Epithelial Cells/ultrastructure , Feces/virology , Humans , Immunohistochemistry , Inclusion Bodies/ultrastructure , Keratins/metabolism , RNA, Double-Stranded/analysis , RNA, Double-Stranded/isolation & purification , RNA, Viral/analysis , Reverse Transcriptase Polymerase Chain Reaction , Rotavirus/immunology , Urine/cytology , Urine/virologyABSTRACT
Regioisomeric 6(1),6n-di-O-trityl-cyclomaltohexaoses (cG6s) or -cyclomaltoheptaoses (cG7s) were prepared by the reaction of cyclomaltohexaose (1, cG6) or cylomaltoheptaose (5, cG7) with chlorotriphenyl-methane in pyridine and isolation by h.p.l.c. The regiochemical determination of each three ditrityl-substituted derivatives has been accomplished by the "hex-5-enose degradation", followed by measurement of their f.a.b.-mass spectra.
