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Sensors (Basel) ; 23(4)2023 Feb 10.
Article in English | MEDLINE | ID: mdl-36850595

ABSTRACT

This paper considers the evaluation of uncertainty of quantitative gel electrophoresis. To date, such uncertainty estimation presented in the literature are based on the multiple measurements performed for assessing the intra- and interlaboratory reproducibility using standard samples. This paper shows how to estimate the uncertainty in cases where we cannot study scattering components of the results. The first point is dedicated to a case where we have standard samples (the direct expressions are shown). The second point considers the situation when standard samples are absent (the algorithm for estimating the lower bound for uncertainty is discussed). The role of the data processing algorithm is demonstrated.


Subject(s)
Nucleic Acids , Reproducibility of Results , Uncertainty , Electrophoresis, Agar Gel , Algorithms
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