1.
Proc Natl Acad Sci U S A
; 96(12): 6999-7004, 1999 Jun 08.
Article
in English
| MEDLINE
| ID: mdl-10359828
ABSTRACT
To study intracellular pathways by which the human papillomavirus 16 oncogene E7 participates in carcinogenesis, we expressed an inducible chimera of E7 by fusion to the hormone-binding domain of the estrogen receptor. The chimeric protein (E7ER) transformed rodent fibroblast cell lines and induced DNA synthesis on addition of estradiol. In coimmunoprecipitation experiments, E7ER preferentially bound p130 when compared to p107 and pRb. After estradiol addition, E7ER localization changed to a more intense intranuclear staining. Induction of E7 function was not correlated with binding to p130 or pRb but rather with intranuclear localization and modest induction of binding to p107.
Subject(s)
Cell Transformation, Viral , Oncogene Proteins, Viral/genetics , Papillomaviridae , Phosphoproteins/genetics , Proteins , Retinoblastoma Protein/genetics , Cell Nucleus/genetics , Cell Nucleus/virology , Humans , Oncogene Proteins, Viral/metabolism , Papillomavirus E7 Proteins , Phosphoproteins/metabolism , Retinoblastoma Protein/metabolism , Retinoblastoma-Like Protein p130 , Transcription Factors/genetics , Transcription Factors/metabolism
2.
Cold Spring Harb Symp Quant Biol
; 59: 491-9, 1994.
Article
in English
| MEDLINE
| ID: mdl-7587104